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    Lipid Exchange and Flip-Flop in Solid Supported Bilayers
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    † Institut Laue-Langevin, 6, rue Jules Horowitz, 38042, Grenoble CEDEX 9, France
    ‡ Department of Chemistry, University of Naples Federico II, Corso Umberto I, 40, 80138 Naples, Italy
    *E-mail: [email protected]
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    Langmuir

    Cite this: Langmuir 2013, 29, 41, 12762–12769
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    https://doi.org/10.1021/la402708u
    Published September 17, 2013
    Copyright © 2013 American Chemical Society
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    Abstract

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    Inter- and intrabilayer transfer of phospholipid molecules was investigated by neutron reflectometry. The structure of solid supported lipid bilayers exposed to a solution of isotopically labeled vesicles was monitored as a function of temperature, time, and vesicle concentration. Lipid interbilayer exchange was shown to be the time limiting process, while lipid intrabilayer movement, the so-called flip-flop, was too fast to be visualized within the experimental acquisition time. The exchange process was characterized by an Arrhenius-like behavior and the activation energy of the process was concentration-independent. The results are discussed and compared extensively with the literature available on the topic.

    Copyright © 2013 American Chemical Society

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    The α(t,T) curves obtained on different instruments were highly reproducible. This material is available free of charge via the Internet at http://pubs.acs.org/.

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    Langmuir

    Cite this: Langmuir 2013, 29, 41, 12762–12769
    Click to copy citationCitation copied!
    https://doi.org/10.1021/la402708u
    Published September 17, 2013
    Copyright © 2013 American Chemical Society
    Request reuse permissions

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