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Protein Micropatterns by PEG Grafting on Dewetted PLGA Films

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† School of Chemistry, The University of Sydney, Sydney, NSW 2006, Australia
‡ CSIRO Materials Science and Engineering, Clayton South, VIC 3169, Australia
§ Centre for Advanced Macromolecular Design (CAMD), The University of New South Wales, Sydney, NSW 2052, Australia
*E-mail: [email protected]
Cite this: Langmuir 2014, 30, 39, 11714–11722
Publication Date (Web):September 8, 2014
https://doi.org/10.1021/la5018592
Copyright © 2014 American Chemical Society
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    Abstract

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    The ability to control protein and cell positioning on a microscopic scale is crucial in many biomedical applications, such as single cell studies. We have developed and investigated the grafting of poly(ethylene glycol) (PEG) brushes onto poly(d,l-lactide-co-glycolide) (PLGA) thin films, which can be micropatterned by exploiting their spontaneous dewetting on top of polystyrene (PS) films. Dense PEG brushes with excellent protein repellence were achieved on PLGA by using cloud point grafting conditions, and selective adsorption of proteins on the micropatterned substrates was achieved by exploiting the different affinity protein adsorption onto the PEG brushes and the PS holes. PEG-grafted PLGA films showed better resistance against spontaneous degradation in buffer than bare PLGA films, due to passivation by the thin PEG coating. The simplicity of dewetting and subsequent grafting approaches, coupled with the ability to coat and pattern nonplanar substrates give rise to possible applications of PEG-grafted PLGA films in single cell studies and cell cultures for tissue engineering.

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    Calculation of PEG grafting density. DSC of PLGA. Dynamics of hole growth in PLGA films. XPS analysis, AFM topography images, and optical micrographs of the employed surfaces. Contact angle measurements. This material is available free of charge via the Internet at http://pubs.acs.org.

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