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Reactive Surface Coatings Based on Polysilsesquioxanes: Controlled Functionalization for Specific Protein Immobilization

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    Reactive Surface Coatings Based on Polysilsesquioxanes: Controlled Functionalization for Specific Protein Immobilization
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    † Institute of Organic Chemistry, University of Mainz, Duesbergweg 10-14, 55099 Mainz, Germany
    ‡ Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany
    *To whom correspondence should be addressed. E-mail: [email protected]. Telephone: +49-6131-3926256. Fax: +49-6131-3924778.
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    Langmuir

    Cite this: Langmuir 2009, 25, 17, 10068–10076
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    https://doi.org/10.1021/la901878h
    Published July 2, 2009
    Copyright © 2009 American Chemical Society
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    Abstract

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    The key designing in reliable biosensors is the preparation of thin films in which biomolecular functions may be immobilized and addressed in a controlled and reproducible manner. This requires the controlled preparation of specific binding sites on planar surfaces. Poly(methylsilsesquioxane)-poly(pentafluorophenyl acrylates) (PMSSQ-PFPA) are promising materials to produce stable and adherent thin reactive coatings on various substrates. Those reactive surface coatings could be applied onto various materials, for example, gold, polycarbonate (PC), poly(tetrafluoroethylene) (PTFE), and glass. By dipping those substrates in a solution of a desired amine, specific binding sites for protein adsorption could be immobilized on the surface. The versatile strategy allowed the attachment of various linkers, for example, biotin, l-thyroxine, and folic acid. The adsorption processes of streptavidin, pre-albumin, and folate-binding protein were monitored using surface plasmon resonance (SPR), Fourier transform infrared (FTIR) spectroscopy, fluorescence spectroscopy, and atomic force microscopy (AFM). The presented protein immobilization strategy, consisting of four steps (a) spin-coating of PMSSQ-PFPA hybrid polymer from tetrahydrofuran (THF) solution, (b) annealing at 130 °C for 2 h to induce thermal cross-linking of the PMSSQ part, (c) surface analogues reaction with different amino-functionalized specific binding sites for proteins, and (d) controlled assembly of proteins on the surface, may find various applications in future biosensor design.

    Copyright © 2009 American Chemical Society

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    AFM topographies of PMSSQ-PFPA coatings on Au, PC, PDMS, and PMMA, SPR kinetic of H2N-biotin attachment on the reactive coating, SPR scans of the streptavidin adsorption, SPR kinetic of streptavidin adsorption on different surfaces, FTIR spectrum of streptavidin immobilized on PC, fluorescence spectra of streptavidin fluorescent polymer on various substrates, SPR kinetics of albumin and pre-albumin on a biotinylated surface, SPR kinetic of T4-disulfide SAM formation and further pre-albumin adsorption, SPR kinetics of streptavidin and albumin on a T4-functionalized surface, and SPR kinetics of albumin and streptavidin on a folic-acid-functionalized surface. This material is available free of charge via the Internet at http://pubs.acs.org.

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    Langmuir

    Cite this: Langmuir 2009, 25, 17, 10068–10076
    Click to copy citationCitation copied!
    https://doi.org/10.1021/la901878h
    Published July 2, 2009
    Copyright © 2009 American Chemical Society
    Request reuse permissions

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