ACS Publications. Most Trusted. Most Cited. Most Read
My Activity

Single Molecular Imaging and Spectroscopy of Conjugated Polyelectrolytes Decorated on Stretched Aligned DNA

View Author Information
Biomolecular and Organic Electronics, Department of Physics, Chemistry and Biology, Linköping University, SE-581 83 Linköping, Sweden, and Department of Chemical Physics, University of Lund, P.O. Box 124, SE-22100, Sweden
Cite this: Nano Lett. 2005, 5, 10, 1948–1953
Publication Date (Web):September 13, 2005
Copyright © 2005 American Chemical Society

    Article Views





    Other access options
    Supporting Info (1)»


    Abstract Image

    DNA is the prototype template for building nanoelectronic devices by self-assembly. The electronic functions are made possible by coordinating electronic polymer chains to DNA. This paper demonstrates two methods for fabrication of aligned and ordered DNA nanowires complexed with conjugated polyelectrolytes (CPEs). The complex can be formed either in solution prior to stretching or after stretching of the bare DNA on a surface. Molecular combing was used to stretch the complexes on surface energy patterned surfaces, and PMMA for the bare DNA. Single molecular spectroscopy, in fluorescence, and microscopy, in atomic force microscopy, give evidence for coordination of the short CPE chains to the aligned DNA.

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.


    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. You can change your affiliated institution below.


     Corresponding author. E-mail:  [email protected]. Phone:  +46 13 282702. Fax:  +46 13 28 89 69.

     Biomolecular and Organic Electronics.

     Department of Chemical Physics.

    Supporting Information Available

    Jump To

    Experimental details describing the PDMS stamp fabrication, an example of stretched bare λ-DNA on a surface-modified substrate, experimental details for the solution fluorescence measurements, and the single molecular spectroscopy setup. This material is available free of charge via the Internet at

    Terms & Conditions

    Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system:

    Cited By

    This article is cited by 35 publications.

    1. Jung Yong Kim, Selvakumaran Nagamani, Lianlian Liu, Ahmed H. Elghazaly, Niclas Solin, Olle Inganäs. A DNA and Self-Doped Conjugated Polyelectrolyte Assembled for Organic Optoelectronics and Bioelectronics. Biomacromolecules 2020, 21 (3) , 1214-1221.
    2. Felix Sunjoo Kim, Guoqiang Ren, and Samson A. Jenekhe . One-Dimensional Nanostructures of π-Conjugated Molecular Systems: Assembly, Properties, and Applications from Photovoltaics, Sensors, and Nanophotonics to Nanoelectronics. Chemistry of Materials 2011, 23 (3) , 682-732.
    3. Jens A. Wigenius, Karin Magnusson, Per Björk, Olof Andersson and Olle Inganäs . DNA Chips with Conjugated Polyelectrolytes in Resonance Energy Transfer Mode. Langmuir 2010, 26 (5) , 3753-3759.
    4. Hongzhen Lin, Seyed R. Tabaei, Daniel Thomsson, Oleg Mirzov, Per-Olof Larsson and Ivan G. Scheblykin . Fluorescence Blinking, Exciton Dynamics, and Energy Transfer Domains in Single Conjugated Polymer Chains. Journal of the American Chemical Society 2008, 130 (22) , 7042-7051.
    5. Mahiar Hamedi, Anna Herland, Roger H. Karlsson and Olle Inganäs . Electrochemical Devices Made from Conducting Nanowire Networks Self-Assembled from Amyloid Fibrils and Alkoxysulfonate PEDOT. Nano Letters 2008, 8 (6) , 1736-1740.
    6. Konstantin Demidenok,, Vera Bocharova,, Manfred Stamm,, Evelin Jähne,, Hans-Jürgen P. Adler, and, Anton Kiriy. One-Dimensional SAMs of (12-Pyrrol-1-yl-dodecyl)-phosphonic Acid Templated by Polyelectrolyte Molecules. Langmuir 2007, 23 (18) , 9287-9292.
    7. Joseph M. Kinsella and, Albena Ivanisevic. DNA-Templated Magnetic Nanowires with Different Compositions:  Fabrication and Analysis. Langmuir 2007, 23 (7) , 3886-3890.
    8. Christian Müller, Liangqi Ouyang, Anja Lund, Kasper Moth‐Poulsen, Mahiar M. Hamedi. From Single Molecules to Thin Film Electronics, Nanofibers, e‐Textiles and Power Cables: Bridging Length Scales with Organic Semiconductors. Advanced Materials 2019, 31 (22)
    9. Aniruddha Kundu, Sudipta Nandi, Arun K. Nandi. Nucleic acid based polymer and nanoparticle conjugates: Synthesis, properties and applications. Progress in Materials Science 2017, 88 , 136-185.
    10. Dong Geun Bae, Ji‐Eun Jeong, Seok Hee Kang, Myunghwan Byun, Dong‐Wook Han, Zhiqun Lin, Han Young Woo, Suck Won Hong. A Nonconventional Approach to Patterned Nanoarrays of DNA Strands for Template‐Assisted Assembly of Polyfluorene Nanowires. Small 2016, 12 (31) , 4254-4263.
    11. Alexandre Garreau, Jean‐Luc Duvail. Recent Advances in Optically Active Polymer‐Based Nanowires and Nanotubes. Advanced Optical Materials 2014, 2 (12) , 1122-1140.
    12. Mahiar Hamedi, Anders Elfwing, Roger Gabrielsson, Olle Inganäs. Electronic Polymers and DNA Self‐Assembled in Nanowire Transistors. Small 2013, 9 (3) , 363-368.
    13. Christian Müller, Olle Inganäs. Bio‐Based Materials as Templates for Electronic Devices. 2012, 401-429.
    14. Éven J. Lemieux, Mario Leclerc. Sensing via Conformational Changes of Conjugated Polythiophenes. 2012, 231-261.
    15. Kangwon Lee, Hyong‐Jun Kim, Jinsang Kim. Design Principle of Conjugated Polyelectrolytes to Make Them Water‐Soluble and Highly Emissive. Advanced Functional Materials 2012, 22 (5) , 1076-1086.
    16. Daniel Aili, Bo Liedberg. Biomimetic Approaches to Self‐Assembly of Nanomaterials. 2010
    17. Jens Wigenius, Per Björk, Mahiar Hamedi, Daniel Aili. Supramolecular Assembly of Designed α‐Helical Polypeptide‐Based Nanostructures and Luminescent Conjugated Polyelectrolytes. Macromolecular Bioscience 2010, 10 (8) , 836-841.
    18. Feng Li, Ganesan Palaniswamy, Menno R. de Jong, Andreas Åslund, Peter Konradsson, Antonius T. M. Marcelis, Ernst J. R. Sudhölter, Martien A. Cohen Stuart, Frans A. M. Leermakers. Nanowires Formed by the Co‐Assembly of a Negatively Charged Low‐Molecular Weight Gelator and a Zwitterionic Polythiophene. ChemPhysChem 2010, 11 (9) , 1956-1960.
    19. Daniel Thomsson, Hongzhen Lin, Ivan G. Scheblykin. Correlation Analysis of Fluorescence Intensity and Fluorescence Anisotropy Fluctuations in Single‐Molecule Spectroscopy of Conjugated Polymers. ChemPhysChem 2010, 11 (4) , 897-904.
    20. Mahiar Hamedi, Jens Wigenius, Feng-I. Tai, Per Björk, Daniel Aili. Polypeptide-guided assembly of conducting polymer nanocomposites. Nanoscale 2010, 2 (10) , 2058.
    21. Oleg Mirzov, Robbert Bloem, Peter Ralph Hania, Daniel Thomsson, Hongzhen Lin, Ivan G. Scheblykin. Polarization Portraits of Single Multichromophoric Systems: Visualizing Conformation and Energy Transfer. Small 2009, 5 (16) , 1877-1888.
    22. Per Björk, Daniel Thomsson, Oleg Mirzov, Jens Wigenius, Olle Inganäs, Ivan G. Scheblykin. Oligothiophene Assemblies Defined by DNA Interaction: From Single Chains to Disordered Clusters. Small 2009, 5 (1) , 96-103.
    23. Reza Mohammadzadegan, Hassan Mohabatkar, Mohammad Hossein Sheikhi, Afsaneh Safavi, Mahmood Barati Khajouee. DNA-templated gold nanowires. Physica E: Low-dimensional Systems and Nanostructures 2008, 41 (1) , 142-145.
    24. Anna Herland, Daniel Thomsson, Oleg Mirzov, Ivan G. Scheblykin, Olle Inganäs. Decoration of amyloid fibrils with luminescent conjugated polymers. J. Mater. Chem. 2008, 18 (1) , 126-132.
    25. P. Szymczak, Marek Cieplak. Proteins in a shear flow. The Journal of Chemical Physics 2007, 127 (15)
    26. Jerrod J. Schwartz, Stephen R. Quake. High density single molecule surface patterning with colloidal epitaxy. Applied Physics Letters 2007, 91 (8)
    27. Venkat Ram Dukkipati, Stella W. Pang. Precise DNA placement and stretching in electrode gaps using electric fields in a microfluidic system. Applied Physics Letters 2007, 90 (8)
    28. Anna Herland, Per Björk, P. Ralph Hania, Ivan G. Scheblykin, Olle Inganäs. Alignment of a Conjugated Polymer onto Amyloid‐like Protein Fibrils. Small 2007, 3 (2) , 318-325.
    29. M. Forster, D. Thomsson, P. R. Hania, I. G. Scheblykin. Redistribution of emitting state population in conjugated polymers probed by single-molecule fluorescence polarization spectroscopy. Phys. Chem. Chem. Phys. 2007, 9 (6) , 761-766.
    30. P. Szymczak, Marek Cieplak. Stretching of proteins in a uniform flow. The Journal of Chemical Physics 2006, 125 (16)
    31. Cerasela Zoica Dinu, Jörg Opitz, Wolfgang Pompe, Jonathon Howard, Michael Mertig, Stefan Diez. Parallel Manipulation of Bifunctional DNA Molecules on Structured Surfaces Using Kinesin‐Driven Microtubules. Small 2006, 2 (8-9) , 1090-1098.
    32. Per Björk, Sven Holmström, Olle Inganäs. Soft Lithographic Printing of Patterns of Stretched DNA and DNA/Electronic Polymer Wires by Surface‐Energy Modification and Transfer. Small 2006, 2 (8-9) , 1068-1074.
    33. Eva Bystrenova, Massimo Facchini, Massimiliano Cavallini, Marcello G. Cacace, Fabio Biscarini. Multiple Length‐Scale Patterning of DNA by Stamp‐Assisted Deposition. Angewandte Chemie 2006, 118 (29) , 4897-4900.
    34. Eva Bystrenova, Massimo Facchini, Massimiliano Cavallini, Marcello G. Cacace, Fabio Biscarini. Multiple Length‐Scale Patterning of DNA by Stamp‐Assisted Deposition. Angewandte Chemie International Edition 2006, 45 (29) , 4779-4782.
    35. Holger Frey, Franziska Gröhn, Andreas Kilbinger. Makromolekulare Chemie 2005. Nachrichten aus der Chemie 2006, 54 (3) , 292-300.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    You’ve supercharged your research process with ACS and Mendeley!

    STEP 1:
    Click to create an ACS ID

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Your Mendeley pairing has expired. Please reconnect