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Download Hi-Res ImageDownload to MS-PowerPointCite This:Nano Lett. 2012, 12, 12, 6453-6458
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Direct Imaging of DNA Fibers: The Visage of Double Helix

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†Nanostructures, §Neuroscience and Brain Technologies, and ⊥Nanochemistry Departments, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy
‡ BIONEM, Bio-Nanotechnology and Engineering for Medicine, Department of experimental and clinical medicine, University of Magna Graecia Viale Europa, Germaneto, 88100 Catanzaro, Italy
∥ IMEM-CNR, Parco Area delle Scienze 37/A, 43124 Parma, Italy
*E-mail: [email protected]
Cite this: Nano Lett. 2012, 12, 12, 6453–6458
Publication Date (Web):November 22, 2012
https://doi.org/10.1021/nl3039162
Copyright © 2012 American Chemical Society
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Abstract

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Direct imaging becomes important when the knowledge at few/single molecule level is requested and where the diffraction does not allow to get structural and functional information. Here we report on the direct imaging of double stranded (ds) λ-DNA in the A conformation, obtained by combining a novel sample preparation method based on super hydrophobic DNA molecules self-aggregation process with transmission electron microscopy (TEM). The experimental breakthrough is the production of robust and highly ordered paired DNA nanofibers that allowed its direct TEM imaging and the double helix structure revealing.

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