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Ultrasensitive and Selective Recognition of Peptide Hormone Using Close-Packed Arrays of hPTHR-Conjugated Polymer Nanoparticles

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    Ultrasensitive and Selective Recognition of Peptide Hormone Using Close-Packed Arrays of hPTHR-Conjugated Polymer Nanoparticles
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    † World Class University Program of Chemical Convergence for Energy & Environment, School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Korea
    ‡ School of Chemical and Biological Engineering, Bio-MAX Institute, Seoul National University, Seoul 151-744, Korea
    § Department of Polymer and Fiber System Engineering, Chonnam National University, Gwangju 500-757, Korea
    *Address correspondence to [email protected], [email protected]
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    ACS Nano

    Cite this: ACS Nano 2012, 6, 6, 5549–5558
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    https://doi.org/10.1021/nn301482x
    Published May 31, 2012
    Copyright © 2012 American Chemical Society
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    Abstract

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    Recognition of diverse hormones in the human body is a highly significant challenge because numerous diseases can be affected by hormonal imbalances. However, the methodologies reported to date for detecting hormones have exhibited limited performance. Therefore, development of innovative methods is still a major concern in hormone-sensing applications. In this study, we report an immobilization-based approach to facilitate formation of close-packed arrays of carboxylated polypyrrole nanoparticles (CPPyNPs) and their integration with human parathyroid hormone receptor (hPTHR), which is a B-class family of G-protein-coupled receptors (GPCRs). Our devices enabled use of an electrically controllable liquid-ion-gated field-effect transistor by using the surrounding phosphate-buffered saline solution (pH 7.4) as electrolyte solution. Field-induced signals from the peptide hormone sensors were observed and provided highly sensitive and selective recognition of target molecules at unprecedentedly low concentrations (ca. 48 fM). This hormone sensor also showed long-term stability and excellent selectivity in fetal bovine serum. Importantly, the hormone receptor attached on the surface of CPPyNPs enabled GPCR functional studies; synergistic effects corresponding to increased hPTH peptide length were monitored. These results demonstrate that close-packed CPPyNP arrays are a promising approach for high-performance biosensing devices.

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    Additional information about sample preparation, characterization, and physicochemical properties, XPS, SEM, and fluorescence images. This material is available free of charge via the Internet at http://pubs.acs.org.

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    ACS Nano

    Cite this: ACS Nano 2012, 6, 6, 5549–5558
    Click to copy citationCitation copied!
    https://doi.org/10.1021/nn301482x
    Published May 31, 2012
    Copyright © 2012 American Chemical Society
    Request reuse permissions

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