ACS Publications. Most Trusted. Most Cited. Most Read
My Activity
CONTENT TYPES

Ultrasensitive and Selective Recognition of Peptide Hormone Using Close-Packed Arrays of hPTHR-Conjugated Polymer Nanoparticles

View Author Information
World Class University Program of Chemical Convergence for Energy & Environment, School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Korea
School of Chemical and Biological Engineering, Bio-MAX Institute, Seoul National University, Seoul 151-744, Korea
§ Department of Polymer and Fiber System Engineering, Chonnam National University, Gwangju 500-757, Korea
*Address correspondence to [email protected], [email protected]
Cite this: ACS Nano 2012, 6, 6, 5549–5558
Publication Date (Web):May 31, 2012
https://doi.org/10.1021/nn301482x
Copyright © 2012 American Chemical Society

    Article Views

    1471

    Altmetric

    -

    Citations

    LEARN ABOUT THESE METRICS
    Other access options
    Supporting Info (1)»

    Abstract

    Abstract Image

    Recognition of diverse hormones in the human body is a highly significant challenge because numerous diseases can be affected by hormonal imbalances. However, the methodologies reported to date for detecting hormones have exhibited limited performance. Therefore, development of innovative methods is still a major concern in hormone-sensing applications. In this study, we report an immobilization-based approach to facilitate formation of close-packed arrays of carboxylated polypyrrole nanoparticles (CPPyNPs) and their integration with human parathyroid hormone receptor (hPTHR), which is a B-class family of G-protein-coupled receptors (GPCRs). Our devices enabled use of an electrically controllable liquid-ion-gated field-effect transistor by using the surrounding phosphate-buffered saline solution (pH 7.4) as electrolyte solution. Field-induced signals from the peptide hormone sensors were observed and provided highly sensitive and selective recognition of target molecules at unprecedentedly low concentrations (ca. 48 fM). This hormone sensor also showed long-term stability and excellent selectivity in fetal bovine serum. Importantly, the hormone receptor attached on the surface of CPPyNPs enabled GPCR functional studies; synergistic effects corresponding to increased hPTH peptide length were monitored. These results demonstrate that close-packed CPPyNP arrays are a promising approach for high-performance biosensing devices.

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.

    Recommended

    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. You can change your affiliated institution below.

    Supporting Information

    ARTICLE SECTIONS
    Jump To

    Additional information about sample preparation, characterization, and physicochemical properties, XPS, SEM, and fluorescence images. This material is available free of charge via the Internet at http://pubs.acs.org.

    Terms & Conditions

    Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system: http://pubs.acs.org/page/copyright/permissions.html.

    Cited By

    This article is cited by 50 publications.

    1. Yi Wan, Chengli Zong, Xiangpeng Li, Aimin Wang, Yan Li, Tao Yang, Qing Bao, Michael Dubow, Mingying Yang, Ledesma-Amaro Rodrigo, Chuanbin Mao. New Insights for Biosensing: Lessons from Microbial Defense Systems. Chemical Reviews 2022, 122 (9) , 8126-8180. https://doi.org/10.1021/acs.chemrev.1c01063
    2. Yoav Y. Broza, Xi Zhou, Miaomiao Yuan, Danyao Qu, Youbing Zheng, Rotem Vishinkin, Muhammad Khatib, Weiwei Wu, Hossam Haick. Disease Detection with Molecular Biomarkers: From Chemistry of Body Fluids to Nature-Inspired Chemical Sensors. Chemical Reviews 2019, 119 (22) , 11761-11817. https://doi.org/10.1021/acs.chemrev.9b00437
    3. Jungkyun Oh, Heehong Yang, Go Een Jeong, Dongseok Moon, Oh Seok Kwon, Sooyeol Phyo, Jiwon Lee, Hyun Seok Song, Tai Hyun Park, Jyongsik Jang. Ultrasensitive, Selective, and Highly Stable Bioelectronic Nose That Detects the Liquid and Gaseous Cadaverine. Analytical Chemistry 2019, 91 (19) , 12181-12190. https://doi.org/10.1021/acs.analchem.9b01068
    4. Oh Seok Kwon, Hyun Seok Song, Tai Hyun Park, Jyongsik Jang. Conducting Nanomaterial Sensor Using Natural Receptors. Chemical Reviews 2019, 119 (1) , 36-93. https://doi.org/10.1021/acs.chemrev.8b00159
    5. Seon Joo Park, Heehong Yang, Seung Hwan Lee, Hyun Seok Song, Chul Soon Park, Joonwon Bae, Oh Seok Kwon, Tai Hyun Park, and Jyongsik Jang . Dopamine Receptor D1 Agonism and Antagonism Using a Field-Effect Transistor Assay. ACS Nano 2017, 11 (6) , 5950-5959. https://doi.org/10.1021/acsnano.7b01722
    6. Beatriz Pelaz, Christoph Alexiou, Ramon A. Alvarez-Puebla, Frauke Alves, Anne M. Andrews, Sumaira Ashraf, Lajos P. Balogh, Laura Ballerini, Alessandra Bestetti, Cornelia Brendel, Susanna Bosi, Monica Carril, Warren C. W. Chan, Chunying Chen, Xiaodong Chen, Xiaoyuan Chen, Zhen Cheng, Daxiang Cui, Jianzhong Du, Christian Dullin, Alberto Escudero, Neus Feliu, Mingyuan Gao, Michael George, Yury Gogotsi, Arnold Grünweller, Zhongwei Gu, Naomi J. Halas, Norbert Hampp, Roland K. Hartmann, Mark C. Hersam, Patrick Hunziker, Ji Jian, Xingyu Jiang, Philipp Jungebluth, Pranav Kadhiresan, Kazunori Kataoka, Ali Khademhosseini, Jindřich Kopeček, Nicholas A. Kotov, Harald F. Krug, Dong Soo Lee, Claus-Michael Lehr, Kam W. Leong, Xing-Jie Liang, Mei Ling Lim, Luis M. Liz-Marzán, Xiaowei Ma, Paolo Macchiarini, Huan Meng, Helmuth Möhwald, Paul Mulvaney, Andre E. Nel, Shuming Nie, Peter Nordlander, Teruo Okano, Jose Oliveira, Tai Hyun Park, Reginald M. Penner, Maurizio Prato, Victor Puntes, Vincent M. Rotello, Amila Samarakoon, Raymond E. Schaak, Youqing Shen, Sebastian Sjöqvist, Andre G. Skirtach, Mahmoud G. Soliman, Molly M. Stevens, Hsing-Wen Sung, Ben Zhong Tang, Rainer Tietze, Buddhisha N. Udugama, J. Scott VanEpps, Tanja Weil, Paul S. Weiss, Itamar Willner, Yuzhou Wu, Lily Yang, Zhao Yue, Qian Zhang, Qiang Zhang, Xian-En Zhang, Yuliang Zhao, Xin Zhou, and Wolfgang J. Parak . Diverse Applications of Nanomedicine. ACS Nano 2017, 11 (3) , 2313-2381. https://doi.org/10.1021/acsnano.6b06040
    7. Girija Thesma Chandran, Xiaowei Li, Alana Ogata, and Reginald M. Penner . Electrically Transduced Sensors Based on Nanomaterials (2012–2016). Analytical Chemistry 2017, 89 (1) , 249-275. https://doi.org/10.1021/acs.analchem.6b04687
    8. Seon Joo Park, Seung Hwan Lee, Heehong Yang, Chul Soon Park, Chang-Soo Lee, Oh Seok Kwon, Tai Hyun Park, and Jyongsik Jang . Human Dopamine Receptor-Conjugated Multidimensional Conducting Polymer Nanofiber Membrane for Dopamine Detection. ACS Applied Materials & Interfaces 2016, 8 (42) , 28897-28903. https://doi.org/10.1021/acsami.6b10437
    9. Min-Sik Kim, Saerona Kim, Hye Jeong Kong, Oh Seok Kwon, and Hyeonseok Yoon . Tunable Electrical-Sensing Performance of Random-Alternating Layered Graphene/Polyaniline Nanoarchitectures. The Journal of Physical Chemistry C 2016, 120 (32) , 18289-18295. https://doi.org/10.1021/acs.jpcc.6b03705
    10. Oh Seok Kwon, Hyun Seok Song, João Conde, Hyoung-il Kim, Natalie Artzi, and Jae-Hong Kim . Dual-Color Emissive Upconversion Nanocapsules for Differential Cancer Bioimaging In Vivo. ACS Nano 2016, 10 (1) , 1512-1521. https://doi.org/10.1021/acsnano.5b07075
    11. Minkyu Kim, Choonghyeon Lee, Young Deok Seo, Sunghun Cho, Jihoo Kim, Gyeongseop Lee, Yun Ki Kim, and Jyongsik Jang . Fabrication of Various Conducting Polymers Using Graphene Oxide as a Chemical Oxidant. Chemistry of Materials 2015, 27 (18) , 6238-6248. https://doi.org/10.1021/acs.chemmater.5b01408
    12. Oh Seok Kwon, Jae-Hyuk Kim, Jin Ku Cho, and Jae-Hong Kim . Triplet–Triplet Annihilation Upconversion in CdS-Decorated SiO2 Nanocapsules for Sub-Bandgap Photocatalysis. ACS Applied Materials & Interfaces 2015, 7 (1) , 318-325. https://doi.org/10.1021/am506233h
    13. Jin Wook Park, Seon Joo Park, Oh Seok Kwon, Choonghyeon Lee, and Jyongsik Jang . Polypyrrole Nanotube Embedded Reduced Graphene Oxide Transducer for Field-Effect Transistor-Type H2O2 Biosensor. Analytical Chemistry 2014, 86 (3) , 1822-1828. https://doi.org/10.1021/ac403770x
    14. Ji Hyun An, Seon Joo Park, Oh Seok Kwon, Joonwon Bae, and Jyongsik Jang . High-Performance Flexible Graphene Aptasensor for Mercury Detection in Mussels. ACS Nano 2013, 7 (12) , 10563-10571. https://doi.org/10.1021/nn402702w
    15. Jun Seop Lee, Dong Hoon Shin, Jaemoon Jun, and Jyongsik Jang . Multidimensional Polypyrrole/Iron Oxyhydroxide Hybrid Nanoparticles for Chemical Nerve Gas Agent Sensing Application. ACS Nano 2013, 7 (11) , 10139-10147. https://doi.org/10.1021/nn404353w
    16. Hyun Seok Song, Oh Seok Kwon, Sang Hun Lee, Seon Joo Park, Un-Kyung Kim, Jyongsik Jang, and Tai Hyun Park . Human Taste Receptor-Functionalized Field Effect Transistor as a Human-Like Nanobioelectronic Tongue. Nano Letters 2013, 13 (1) , 172-178. https://doi.org/10.1021/nl3038147
    17. Seongyeon Cho, Haein Lee, Yong Hee Han, Tae Shin Park, Sang Woo Seo, Tai Hyun Park. Design of an effective small expression tag to enhance GPCR production in E. coli ‐based cell‐free and whole cell expression systems. Protein Science 2023, 32 (12) https://doi.org/10.1002/pro.4839
    18. Gopal Sanyal, Rimpa Jaiswal, Brahmananda Chakraborty. 2D materials-conducting polymers-based hybrids for electrochemical sensing. 2023, 325-354. https://doi.org/10.1016/B978-0-443-15293-1.00012-4
    19. Lu Hao, Changyi Dong, Lifeng Zhang, Kaiming Zhu, Demei Yu. Polypyrrole Nanomaterials: Structure, Preparation and Application. Polymers 2022, 14 (23) , 5139. https://doi.org/10.3390/polym14235139
    20. Sae Ryun Ahn, Ji Hyun An, Seung Hwan Lee, Hyun Seok Song, Jyongsik Jang, Tai Hyun Park. Peptide hormone sensors using human hormone receptor-carrying nanovesicles and graphene FETs. Scientific Reports 2020, 10 (1) https://doi.org/10.1038/s41598-019-57339-1
    21. Seon Joo Park, Jiyeon Lee, Sung Eun Seo, Kyung Ho Kim, Chul Soon Park, Sang Hun Lee, Hyun Seung Ban, Byoung Dae Lee, Hyun Seok Song, Jinyeong Kim, Chang-Soo Lee, Joonwon Bae, Oh Seok Kwon. High-Performance Conducting Polymer Nanotube-based Liquid-Ion Gated Field-Effect Transistor Aptasensor for Dopamine Exocytosis. Scientific Reports 2020, 10 (1) https://doi.org/10.1038/s41598-020-60715-x
    22. Sunghun Cho, Jun Seop Lee. Recent Development of Morphology Controlled Conducting Polymer Nanomaterial-Based Biosensor. Applied Sciences 2020, 10 (17) , 5889. https://doi.org/10.3390/app10175889
    23. Kyung Ho Kim, Sang Hun Lee, Sung Eun Seo, Joonwon Bae, Seon Joo Park, Oh Seok Kwon. Ultrasensitive Stress Biomarker Detection Using Polypyrrole Nanotube Coupled to a Field-Effect Transistor. Micromachines 2020, 11 (4) , 439. https://doi.org/10.3390/mi11040439
    24. Sang Hun Lee, Kyung Ho Kim, Sung Eun Seo, Mun il Kim, Seon Joo Park, Oh Seok Kwon. Cytochrome C-decorated graphene field-effect transistor for highly sensitive hydrogen peroxide detection. Journal of Industrial and Engineering Chemistry 2020, 83 , 29-34. https://doi.org/10.1016/j.jiec.2019.11.009
    25. Sang Won CHO, Tai Hyun PARK. Recent Advances in the Development of the Bioelectronic Sensor Using Sensory Receptors. Journal of Japan Association on Odor Environment 2020, 51 (1) , 2-8. https://doi.org/10.2171/jao.51.2
    26. Yang Xiao, Hong-Xia Huang, Da-Ming Liang, Cheng Wang. Electrocatalytic properties and modification of La0.6Ca0.4Co1−Mn O3 (x = 0–0.9) perovskite-type oxides. Chemical Physics Letters 2020, 738 , 136846. https://doi.org/10.1016/j.cplett.2019.136846
    27. Nan Li, Ari M. Chow, Hashwin V. S. Ganesh, Melanie Ratnam, Ian R. Brown, Kagan Kerman. Diazonium-Modified Screen-Printed Electrodes for Immunosensing Growth Hormone in Blood Samples. Biosensors 2019, 9 (3) , 88. https://doi.org/10.3390/bios9030088
    28. Ajay Kumar Yagati, Anna Go, Sachin Ganpat Chavan, Changyoon Baek, Min-Ho Lee, Junhong Min. Nanostructured Au-Pt hybrid disk electrodes for enhanced parathyroid hormone detection in human serum. Bioelectrochemistry 2019, 128 , 165-174. https://doi.org/10.1016/j.bioelechem.2019.04.011
    29. Myung Sup Kim, Kyung Hee Cho, Kwang Hyun Park, Jyongsik Jang, Ji-Sook Hahn. Activation of Haa1 and War1 transcription factors by differential binding of weak acid anions in Saccharomyces cerevisiae. Nucleic Acids Research 2019, 47 (3) , 1211-1224. https://doi.org/10.1093/nar/gky1188
    30. Xiu Zhang, Jing Cheng, Lei Wu, Yong Mei, Nicole Jaffrezic-Renault, Zhenzhong Guo. An overview of an artificial nose system. Talanta 2018, 184 , 93-102. https://doi.org/10.1016/j.talanta.2018.02.113
    31. Kyoung Lee, Kyung Kim, Hyeonseok Yoon, Hyungwoo Kim. Chemical Design of Functional Polymer Structures for Biosensors: From Nanoscale to Macroscale. Polymers 2018, 10 (5) , 551. https://doi.org/10.3390/polym10050551
    32. Frank Davis, Flavio M. Shimizu, Zeynep Altintas. Smart Nanomaterials. 2018, 219-276. https://doi.org/10.1002/9781119065036.ch11
    33. Thanh-Hai Le, Yukyung Kim, Hyeonseok Yoon. Electrical and Electrochemical Properties of Conducting Polymers. Polymers 2017, 9 (12) , 150. https://doi.org/10.3390/polym9040150
    34. Duong Nguyen, Hyeonseok Yoon. Recent Advances in Nanostructured Conducting Polymers: from Synthesis to Practical Applications. Polymers 2016, 8 (4) , 118. https://doi.org/10.3390/polym8040118
    35. Dong Hoon Shin, Jun Seop Lee, Jaemoon Jun, Ji Hyun An, Sung Gun Kim, Kyung Hee Cho, Jyongsik Jang. Flower-like Palladium Nanoclusters Decorated Graphene Electrodes for Ultrasensitive and Flexible Hydrogen Gas Sensing. Scientific Reports 2015, 5 (1) https://doi.org/10.1038/srep12294
    36. Ki-Jin Ahn, Younghee Lee, Hojin Choi, Min-Sik Kim, Kyungun Im, Seonmyeong Noh, Hyeonseok Yoon. Surfactant-Templated Synthesis of Polypyrrole Nanocages as Redox Mediators for Efficient Energy Storage. Scientific Reports 2015, 5 (1) https://doi.org/10.1038/srep14097
    37. Heehong Yang, Hyun Seok Song, Sae Ryun Ahn, Tai Hyun Park. Purification and functional reconstitution of human olfactory receptor expressed in Escherichia coli. Biotechnology and Bioprocess Engineering 2015, 20 (3) , 423-430. https://doi.org/10.1007/s12257-014-0897-4
    38. Jin Wook Park, Choonghyeon Lee, Jyongsik Jang. High-performance field-effect transistor-type glucose biosensor based on nanohybrids of carboxylated polypyrrole nanotube wrapped graphene sheet transducer. Sensors and Actuators B: Chemical 2015, 208 , 532-537. https://doi.org/10.1016/j.snb.2014.11.085
    39. Adil Sultan, Sharique Ahmad, Tarique Anwer, Faiz Mohammad. Binary doped polypyrrole and polypyrrole/boron nitride nanocomposites: preparation, characterization and application in detection of liquefied petroleum gas leaks. RSC Advances 2015, 5 (128) , 105980-105991. https://doi.org/10.1039/C5RA21173E
    40. Seon Joo Park, Hyun Seok Song, Oh Seok Kwon, Ji Hyun Chung, Seung Hwan Lee, Ji Hyun An, Sae Ryun Ahn, Ji Eun Lee, Hyeonseok Yoon, Tai Hyun Park, Jyongsik Jang. Human dopamine receptor nanovesicles for gate-potential modulators in high-performance field-effect transistor biosensors. Scientific Reports 2014, 4 (1) https://doi.org/10.1038/srep04342
    41. Jong Hyun Lim, Tai Hyun Park. Concept of Bioelectronic Nose. 2014, 1-22. https://doi.org/10.1007/978-94-017-8613-3_1
    42. Oh Seok Kwon, Jyongsik Jang. Conducting Polymer Nanomaterial-Based Sensor Platform for Bioelectronic Nose. 2014, 243-262. https://doi.org/10.1007/978-94-017-8613-3_13
    43. Hwi Jin Ko, Jong Hyun Lim, Eun Hae Oh, Tai Hyun Park. Applications and Perspectives of Bioelectronic Nose. 2014, 263-283. https://doi.org/10.1007/978-94-017-8613-3_14
    44. Hyun Seok Song, Tai Hyun Park. Production of Olfactory Receptors and Nanovesicles Using Heterologous Cell Systems for Bioelectronic Nose. 2014, 145-170. https://doi.org/10.1007/978-94-017-8613-3_9
    45. Jooyoung Song, Jyongsik Jang. Antimicrobial polymer nanostructures: Synthetic route, mechanism of action and perspective. Advances in Colloid and Interface Science 2014, 203 , 37-50. https://doi.org/10.1016/j.cis.2013.11.007
    46. Long Zhang, Yangping Wen, Yuanyuan Yao, Jingkun Xu, Xuemin Duan, Ge Zhang. Synthesis and Characterization of PEDOT Derivative with Carboxyl Group and Its Chemo/Bio Sensing Application as Nanocomposite, Immobilized Biological and Enhanced Optical Materials. Electrochimica Acta 2014, 116 , 343-354. https://doi.org/10.1016/j.electacta.2013.11.042
    47. Hyeonseok Yoon. Current Trends in Sensors Based on Conducting Polymer Nanomaterials. Nanomaterials 2013, 3 (3) , 524-549. https://doi.org/10.3390/nano3030524
    48. Oh Seok Kwon, Seung Hwan Lee, Seon Joo Park, Ji Hyun An, Hyun Seok Song, Taejoon Kim, Joon Hak Oh, Joonwon Bae, Hyeonseok Yoon, Tai Hyun Park, Jyongsik Jang. Large‐Scale Graphene Micropattern Nano‐biohybrids: High‐Performance Transducers for FET‐Type Flexible Fluidic HIV Immunoassays. Advanced Materials 2013, 25 (30) , 4177-4185. https://doi.org/10.1002/adma.201301523
    49. Wan-Kyu Oh, Oh Seok Kwon, Jyongsik Jang. Conducting Polymer Nanomaterials for Biomedical Applications: Cellular Interfacing and Biosensing. Polymer Reviews 2013, 53 (3) , 407-442. https://doi.org/10.1080/15583724.2013.805771
    50. Jun Seop Lee, Oh Seok Kwon, Dong Hoon Shin, Jyongsik Jang. WO3 nanonodule-decorated hybrid carbon nanofibers for NO2 gas sensor application. Journal of Materials Chemistry A 2013, 1 (32) , 9099. https://doi.org/10.1039/c3ta11658a

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    You’ve supercharged your research process with ACS and Mendeley!

    STEP 1:
    Click to create an ACS ID

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    MENDELEY PAIRING EXPIRED
    Your Mendeley pairing has expired. Please reconnect