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Continuous Flow Atomic Force Microscopy Imaging Reveals Fluidity and Time-Dependent Interactions of Antimicrobial Dendrimer with Model Lipid Membranes

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Nano-Science Center and Institute of Chemistry, Copenhagen University, København DK2100, Denmark
European Spallation Source ESS AB, 221 00 Lund, Sweden
§ Institute of Organic Chemistry, Polish Academy of Science, 01-224 Warszawa, Poland
*Address correspondence to [email protected]
Cite this: ACS Nano 2014, 8, 1, 396–408
Publication Date (Web):December 4, 2013
https://doi.org/10.1021/nn404530z
Copyright © 2013 American Chemical Society
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Abstract

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In this paper, an amphiphilic peptide dendrimer with potential applications against multi-resistant bacteria such as Staphylococcus aureus was synthesized and studied on model cell membranes. The combination of quartz crystal microbalance and atomic force microscopy imaging during continuous flow allowed for in situ monitoring of the very initial interaction processes and membrane transformations on longer time scales. We used three different membrane compositions of low and high melting temperature phospholipids to vary the membrane properties from a single fluid phase to a pure gel phase, while crossing the phase coexistence boundaries at room temperature. The interaction mechanism of the dendrimer was found to be time-dependent and to vary remarkably with the fluidity and coexistence of liquid–solid phases in the membrane. Spherical micelle-like dendrimer–lipid aggregates were formed in the fluid-phase bilayer and led to partial solubilization of the membrane, while in gel-phase membranes, the dendrimers caused areas of local depressions followed by redeposition of flexible lipid patches. Domain coexistence led to a sequence of events initiated by the formation of a ribbon-like network and followed by membrane solubilization via spherical aggregates from the edges of bilayer patches. Our results show that the dendrimer molecules were able to destroy the membrane integrity through different mechanisms depending on the lipid phase and morphology and shed light on their antimicrobial activity. These findings could have an impact on the efficacy of the dendrimers since lipid membranes in certain bacteria have transition temperatures very close to the host body temperature.

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MICs of BALY toward various bacterial strains and the synthetic route to BALY including MSLR, ESI, 1H NMR, and 13C NMR data. QCM-D raw data for BALY interaction with POPC, 80 mol % DPPC, and DPPC. AFM image and height distribution of a DPPC bilayer prior to interaction with BALY. AFM images and height profile of 6 μM BALY interacting with DPPC. Height distribution function of the AFM image in Figure 8G in the main text. AFM image and height profile of 80 mol % DPPC subjected to high scanning force to remove objects formed after 1 day of incubation. This material is available free of charge via the Internet at http://pubs.acs.org.

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