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Screening of Triterpenoids Isolated from Phyllanthus flexuosus for DNA Topoisomerase Inhibitory Activity

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Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan, and Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan
Cite this: J. Nat. Prod. 2001, 64, 12, 1545–1547
Publication Date (Web):November 8, 2001
https://doi.org/10.1021/np010176u
Copyright © 2001 American Chemical Society and American Society of Pharmacognosy
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    Abstract

    DNA topoisomerases (Topos) are enzymes that play a crucial role in DNA metabolism events such as replication, transcription, recombination, and chromosome segregation at mitosis. Thus, Topo inhibitors could be expected to have antitumor effects. Naturally occurring lupane- and oleanane-type triterpenoids isolated from the bark of Phyllanthus flexuosus were screened for human Topos I and II inhibitory activities. Olean-12-en-3β,15α-diol (1), olean-12-en-3β,15α,24-triol (3), lupeol (4), and betulin (6) were found to be selective catalytic inhibitors of human Topo II activity with IC50 values in the range of 10−39 μM.

    *

     To whom correspondence should be addressed. Fax:  (+81)726-90-1005. E-mail:  [email protected].

    †

     Osaka University of Pharmaceutical Sciences.

    ‡

     Graduate School of Pharmaceutical Sciences, Kyoto University.

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