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Protein Synthesis Patterns Reveal a Complex Regulatory Response to Singlet Oxygen in Rhodobacter

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Institut für Mikrobiologie und Molekularbiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26-32, D-35392 Gieβen, Germany, and Max Planck Institut für Biochemie, Am Klopferspitz 18, D-82152 Martinsried, Germany
Cite this: J. Proteome Res. 2007, 6, 7, 2460–2471
Publication Date (Web):May 31, 2007
https://doi.org/10.1021/pr060624p
Copyright © 2007 American Chemical Society

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    Abstract

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    Singlet oxygen (1O2) is a stress factor and signal in the facultative phototrophic bacterium Rhodobacter sphaeroides. In vivo protein labeling with l-[35S]-methionine and analysis by two-dimensional gel electrophoresis revealed that the synthesis of 61 proteins was changed in response to 1O2. After 1O2 treatment, protein synthesis patterns were distinct from those after H2O2 treatment but similar to those after high light exposure. This indicates regulatory mechanisms selective for different reactive oxygen species (ROS) and a response to light partly mediated by 1O2. Analysis of mutant strains support that the response to 1O2 is regulated mainly by rpoE (σE), but also a modulation of the σE dependent response by other factors and the existence of σE independent responses. The involvement of the RNA chaperon Hfq in the 1O2 response implies a role of small regulatory RNAs.

    Keywords: Rhodobacter • singlet oxygen • stress • regulation • protein synthesis • rpoE • sRNA

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     To whom correspondence should be addressed. Mailing address, Institut für Mikrobiologie und Molekularbiologie, Heinrich-Buff-Ring 26-32, 35392 Gieβen, Germany; Phone, (+49) 641 99 355 57; Fax, (+49) 641 99 355 49; E-mail, Jens. [email protected].

     Justus Liebig Universität Giessen.

     Further addresses and phone numbers. Prof. G. Klug:  Phone, (+49) 641 99 355 42; E-mail, [email protected]. M. Zobawa and Dr. F. Lottspeich:  Phone, +49 (89) 8578 2465; Fax, +49 (89) 8578 2802; E-mail, [email protected], [email protected].

    §

     Max Planck Institut für Biochemie.

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    Supporting material includes two tables with the proteins identified in this study. Supplemental Table 1 includes all information on the changes in synthesis for individual proteins by 1O2, high light, and H2O2 in wild-type strain 2.4.1 and for changes in mutant strains TF18 and ΔChrR with and without treatment of 1O2. Proteins have been listed according to the regulatory categories defined in the manuscript. In Supplemental Table 2, all proteins identified are listed according to functional categories. This material is available free of charge via the Internet at http://pubs.acs.org.

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