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Metabonomic Studies of Human Hepatocellular Carcinoma Using High-Resolution Magic-Angle Spinning 1H NMR Spectroscopy in Conjunction with Multivariate Data Analysis

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State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Centre for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, the Chinese Academy of Sciences, Wuhan 430071, P. R. China, Graduate University of the Chinese Academy of Sciences, Beijing 100049, P.R. China, Department of General Surgery, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, P. R. China, Department of General Surgery, Wuxi No.2 People's Hospital, Jiangsu 214002, P. R. China, and Department of Pathology, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, P.R. China
Cite this: J. Proteome Res. 2007, 6, 7, 2605–2614
Publication Date (Web):June 12, 2007
https://doi.org/10.1021/pr070063h
Copyright © 2007 American Chemical Society

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    Abstract

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    High-resolution magic-angle spinning (MAS) 1H nuclear magnetic resonance spectroscopy has been employed to characterize the metabolite composition (i.e., metabonome) of the human hepatocellular carcinoma (HCC) tumor in combination with principal component analysis (PCA). The results showed that (a) the metabonomes of both low-grade HCC and high-grade HCC tumors differ markedly from that of the adjacent non-involved tissues; and (b) low-grade HCC tumors have clear differences in metabonome from that of the high-grade HCC tumors. Compared with the non-involved adjacent liver tissues, HCC tumors had elevated levels of lactate, glutamate, glutamine, glycine, leucine, alanine, choline metabolites, and phosphorylethanolamine (PE), but declined levels of triglycerides, glucose, and glycogen. The levels of lactate, amino acids including glutamate, glutamine, glycine, leucine and alanine, choline and phosphorylethanolamine (PE) were higher but the levels of PC, GPC, triglycerides, glucose, and glycogen were lower in high-grade HCC than in low-grade HCC tumors. Compared with non-cirrhotic, low-grade HCC tumors, the cirrhotic, low-grade HCC tumors showed statistically significant increases in lactate, phosphocholine (PC), and glycerophosphocholine (GPC). The necrosis in HCC tumors resulted in a drastic increase in the levels of observable triglycerides, signals of which dominated their 1H NMR spectra. These results indicated that HRMAS combined with PCA offers a useful tool for understanding the tumor biochemistry and classification of liver tumor tissues; such tool may also have some potential for liver tumor diagnosis and prognosis even when some other disease processes are present.

    Keywords: Magic-angle spinning NMR • Hepatocellular carcinoma • Pattern recognition • metabonomics

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     Wuhan Institute of Physics and Mathematics, the Chinese Academy of Sciences.

     Graduate University of the Chinese Academy of Sciences.

     Department of General Surgery, Union Hospital, Huazhong University of Science and Technology.

    §

     Wuxi No.2 People's Hospital.

     Department of Pathology, Union Hospital, Huazhong University of Science and Technology.

    *

      To whom correspondence should be addressed. E-mails:  huiru.tang@ wipm.ac.cn (H. R.Tang); [email protected] (F. Deng).

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    Spectra of non-involved liver tissues and low- and high-grade HCC tumors. This material is available free of charge via the Internet at http://pubs.acs.org.

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