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Organellar Protein Complexes of Caco-2 Human Cells Analyzed by Two-Dimensional Blue Native/SDS-PAGE and Mass Spectrometry

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    Organellar Protein Complexes of Caco-2 Human Cells Analyzed by Two-Dimensional Blue Native/SDS-PAGE and Mass Spectrometry
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    Laboratoire de Biochimie des Protéines et Protéomique, Université Paris 13, UMR CNRS 7033, 74 rue Marcel Cachin F-93017 Bobigny cedex, France, Institut de Biochimie et Génétique Cellulaires, Université Bordeaux 2, UMR CNRS 5095, 1 rue Camille Saint-Saëns F-33077 Bordeaux Cedex, France, and Laboratoire Polymères, Biopolymères, Surfaces, Equipe BRICS, Université de Rouen, UMR CNRS 6270, Boulevard Maurice de Broglie F-76821 Mont-Saint-Aignan cedex, France
    * To whom correspondence should be addressed. Dr. Jean-Paul Lasserre, phone, +33 (0)556991120; fax, +33 (0)556999059; e-mail, [email protected]. Dr, Julie Hardouin, phone, +33 (0)235146709; fax, +33 (0)235146704; e-mail, [email protected]
    †Université Paris 13.
    ‡Université Bordeaux 2.
    §Université de Rouen.
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    Journal of Proteome Research

    Cite this: J. Proteome Res. 2010, 9, 10, 5093–5107
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    https://doi.org/10.1021/pr100381m
    Published July 22, 2010
    Copyright © 2010 American Chemical Society
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    Abstract

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    The complexome is essential for a better understanding of protein functions. In order to study protein complexes, an approach allowing the extraction and the analysis in native conditions is needed. Two-dimensional blue native/SDS-PAGE (2D BN/SDS-PAGE) technology is thus an interesting and powerful approach for this purpose. This report deals with the analysis and the identification of the organellar protein complexes of Caco-2 human cells using 2D BN/SDS-PAGE and HPLC-chip-MS. We identified 58 protein complexes (26 heteromultimeric and 32 homomultimeric complexes) and 4 monomeric proteins. Among them, 32 protein complexes were pointed out, providing insights into the function of previously uncharacterized human proteins.

    Copyright © 2010 American Chemical Society

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    Cited By

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    This article is cited by 5 publications.

    1. Chengxin He, Liwen Song, Ziwei Liu, Hua Xiong, Qiang Zhao. Effects of stirring speed ladder on the acid-promoted refolding of rice glutelin. International Journal of Biological Macromolecules 2023, 228 , 216-223. https://doi.org/10.1016/j.ijbiomac.2022.12.119
    2. Aldo Jesorka, Inga Põldsalu, Irep Gözen. Microfluidic technology for investigation of protein function in single adherent cells. 2019, 145-172. https://doi.org/10.1016/bs.mie.2019.07.038
    3. Sifeng Mao, Weiwei Li, Qiang Zhang, Wanling Zhang, Qiushi Huang, Jin-Ming Lin. Cell analysis on chip-mass spectrometry. TrAC Trends in Analytical Chemistry 2018, 107 , 43-59. https://doi.org/10.1016/j.trac.2018.06.019
    4. Carla Rossini Crepaldi, Phelipe Augusto Mariano Vitale, Andrea Cristina Tesch, Hélen Julie Laure, José César Rosa, Marcelo de Cerqueira César. Application of 2D BN/SDS-PAGE coupled with mass spectrometry for identification of VDAC-associated protein complexes related to mitochondrial binding sites for type I brain hexokinase. Mitochondrion 2013, 13 (6) , 823-830. https://doi.org/10.1016/j.mito.2013.05.009
    5. Jean Paul Lasserre, Armelle Ménard. Two-Dimensional Blue Native/SDS Gel Electrophoresis of Multiprotein Complexes. 2012, 317-337. https://doi.org/10.1007/978-1-61779-821-4_27
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    Journal of Proteome Research

    Cite this: J. Proteome Res. 2010, 9, 10, 5093–5107
    Click to copy citationCitation copied!
    https://doi.org/10.1021/pr100381m
    Published July 22, 2010
    Copyright © 2010 American Chemical Society
    Request reuse permissions

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