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Analysis of Natural Variation of the Potato Tuber Proteome Reveals Novel Candidate Genes for Tuber Bruising

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Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany
*Claude Urbany, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, Cologne 50829, Germany. Tel: ++49 221 5062−414. Fax: ++49 221 5062-413. E-mail: [email protected]
Cite this: J. Proteome Res. 2012, 11, 2, 703–716
Publication Date (Web):November 2, 2011
https://doi.org/10.1021/pr2006186
Copyright © 2011 American Chemical Society
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Abstract

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Potato (Solanum tuberosum) presents a challenging organism for the genetic and molecular dissection of complex traits due to its tetraploidy and high heterozygosity. One such complex trait of high agronomic interest is the tuber susceptibility to bruising upon mechanical impact, which involves an enzymatic browning reaction. We have compared the tuber proteome of two groups of 10 potato cultivars differing in bruising susceptibility to (i) identify de novo proteins that contribute to bruising, based on differential protein expression, and (ii) validate these proteins by combining proteomics with association genetics. The comparison of 20 potato varieties yields insight into the high natural variation of tuber protein patterns due to genetic background. Seven genes or gene families were found that were both differentially expressed on the protein level between groups and for which DNA polymorphisms were associated with the investigated traits. A putative class III lipase was identified as a novel factor contributing to the natural variation of bruising. Additionally, tuber proteome changes triggered by mechanical impact, within and between groups, were monitored over time. Differentially expressed proteins were found, notably lipases, patatins, and annexins, showing remarkable time-dependent protein variation.

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Table showing the identies and description of all peptides identified in this study. Information on MALDI data acquisition and database significance thresholds. This material is available free of charge via the Internet at http://pubs.acs.org.

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  27. Jeung Joo Lee, Kee Woong Park, Youn-Sig Kwak, Jae Young Ahn, Young Hak Jung, Byung-Hyun Lee, Jae Cheol Jeong, Haeng-Soon Lee, Sang-Soo Kwak. Comparative proteomic study between tuberous roots of light orange- and purple-fleshed sweetpotato cultivars. Plant Science 2012, 193-194 , 120-129. https://doi.org/10.1016/j.plantsci.2012.06.003
  28. C. Gebhardt, C. Urbany, L. Li, B. Stich, J. Paulo, A. Draffehn, A. Ballvora. Molecular Diagnostics for Complex Pest and Disease Resistance and Tuber Quality Traits: Concept, Achievements and Perspectives. Potato Research 2011, 54 (4) , 313-318. https://doi.org/10.1007/s11540-011-9193-2

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