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miRNA and Protein Expression Profiles of Visceral Adipose Tissue Reveal miR-141/YWHAG and miR-520e/RAB11A as Two Potential miRNA/Protein Target Pairs Associated with Severe Obesity

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Fondazione IRCCS SDN, Istituto di Ricerca Diagnostica e Nucleare, Via Gianturco 113, 80143 Naples, Italy
Dipartimento di Biochimica e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Via Pansini 5, 80131 Naples, Italy
§ CEINGE Biotecnologie Avanzate S.C. a R.L., Via Gaetano Salvatore 486, 80145, Naples, Italy
Dipartimento di Chirurgia Generale, Geriatrica, Oncologica e Tecnologie Avanzate, Università degli Studi di Napoli Federico II, Via Pansini 5, 80131 Naples, Italy
*Fax: 0039-081-7462404. Tel.: 0039-081-7463541. E-mail: [email protected]
Cite this: J. Proteome Res. 2012, 11, 6, 3358–3369
Publication Date (Web):April 27, 2012
Copyright © 2012 American Chemical Society

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    Adipose tissues show selective gene expression patterns, to whom microRNAs (miRNAs) may contribute. We evaluated in visceral adipose tissue (VAT) from obese and nonobese females, both miRNA and protein expression profiles, to identify miRNA/protein target pairs associated with obesity (metabolic pathways miRNA-deregulated during obesity). Obese and nonobese females [BMI 42.2 ± 1.6 and 23.7 ± 1.2 kg/m2 (mean ± SEM), respectively] were enrolled in this study. Notably, most miRNAs were down-expressed in obese tissues, whereas most of the proteins from the investigated spots were up-expressed. Bioinformatics integration of miRNA expression and proteomic data highlighted two potential miRNA/protein target pairs: miR-141/YWHAG (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide) and miR-520e/RAB11A (Ras-related protein RAB-11A); the functional interaction between these miRNAs and their target sequences on the corresponding mRNAs was confirmed by luciferase assays. Both RAB11A and YWHAG proteins are involved in glucose homeostasis; YWHAG is also involved in lipid metabolism. Hence, the identified miRNA/protein target pairs are potential players in the obese phenotype.

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    Figure S1: Main bioinformatically predicted pathways that are deregulated by miRNAs in VAT from obese females. Putative target genes of deregulated miRNAs in VAT from obese patients were sorted into pathways using the Kegg database. In parentheses are the number of genes targeted by miRNAs. Table S1: The sequences and charge states of each peptide used for identification of the proteins by LC–MS/MS. This material is available free of charge via the Internet at

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