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Mass++: A Visualization and Analysis Tool for Mass Spectrometry

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Koichi Tanaka Laboratory of Advanced Science and Technology, Shimadzu Corporation, Kyoto 604-8511, Japan
Eisai Product Creation Systems, Eisai Co., Ltd., Tsukuba, Ibaraki 300-2635, Japan
§ iBioTech Co., Tsukuba, Ibaraki 300-0031, Japan
Graduate School of Information Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan
*Phone: +81-75-823-2897. Fax: +81-75-823-2900. E-mail: [email protected]
Cite this: J. Proteome Res. 2014, 13, 8, 3846–3853
Publication Date (Web):June 26, 2014
Copyright © 2014 American Chemical Society

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    Abstract Image

    We have developed Mass++, a plug-in style visualization and analysis tool for mass spectrometry. Its plug-in style enables users to customize it and to develop original functions. Mass++ has several kinds of plug-ins, including rich viewers and analysis methods for proteomics and metabolomics. Plug-ins for supporting vendors’ raw data are currently available; hence, Mass++ can read several data formats. Mass++ is both a desktop tool and a software development platform. Original functions can be developed without editing the Mass++ source code. Here, we present this tool’s capability to rapidly analyze MS data and develop functions by providing examples of label-free quantitation and implementing plug-ins or scripts. Mass++ is freely available at

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    Supporting Information

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    Plug-in structure, Mass++ menus, supported data formats, peak detection algorithms, normalization methods, peak position determination methods, examples of call types in Mass++, MSB file format, results of performance tests for MSB file format, example of Mass++ plug-in development using C#, table of MS data analysis tools, and table of development environments. This material is available free of charge via the Internet at Mass++ runs on 32-bit and 64-bit Windows and can be downloaded free of charge via the Internet at A Mass++ community is operated as a Google Group (

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