ACS Publications. Most Trusted. Most Cited. Most Read
My Activity

Characterization and Modeling of Transcriptional Cross-Regulation in Acinetobacter baylyi ADP1

View Author Information
Kroto Research Institute, University of Sheffield, Broad Lane, Sheffield S3 7HQ, U.K.
BGI-Shenzhen, Shenzhen 518083, P.R. China
§ Shenzhen Key Laboratory of Environmental Microbial Genomics and Application, Shenzhen 518083, P.R. China
Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100029, China
Centre for Ecology and Hydrology, Wallingford, Banson Road, Wallingford OX10 8BB, U.K.
Cite this: ACS Synth. Biol. 2012, 1, 7, 274–283
Publication Date (Web):June 11, 2012
Copyright © 2012 American Chemical Society

    Article Views





    Other access options
    Supporting Info (1)»


    Abstract Image

    Synthetic biology involves reprogramming and engineering of regulatory genes in innovative ways for the implementation of novel tasks. Transcriptional gene regulation systems induced by small molecules in prokaryotes provide a rich source for logic gates. Cross-regulation, whereby a promoter is activated by different molecules or different promoters are activated by one molecule, can be used to design an OR-gate and achieve cross-talk between gene networks in cells. Acinetobacter baylyi ADP1 is naturally transformable, readily editing its chromosomal DNA, which makes it a convenient chassis for synthetic biology. The catabolic genes for salicylate, benzoate, and catechol metabolism are located within a supraoperonic cluster (-sal-are-ben-cat-) in the chromosome of A. baylyi ADP1, which are separately regulated by LysR-type transcriptional regulators (LTTRs). ADP1-based biosensors were constructed in which salA, benA, and catB were fused with a reporter gene cassette luxCDABE under the separate control of SalR, BenM, and CatM regulators. Salicylate, benzoate, catechol, and associated metabolites were found to mediate cross-regulation among sal, ben, and cat operons. A new mathematical model was developed by considering regulator-inducer binding and promoter activation as two separate steps. This model fits the experimental data well and is shown to predict cross-regulation performance.

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.


    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. You can change your affiliated institution below.

    Supporting Information

    Jump To

    This material is available free of charge via the Internet at

    Terms & Conditions

    Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system:

    Cited By

    This article is cited by 14 publications.

    1. Yueqiao Liu, Xiaohui Zhao, Xinzi Wang, Aizhong Ding, Dayi Zhang. Application of whole-cell bioreporters for ecological risk assessment and bioremediation potential evaluation after a benzene exceedance accident in groundwater in Lanzhou, China. Science of The Total Environment 2024, 906 , 167846.
    2. Tingting Teng, Wei E. Huang, Guanghe Li, Xinzi Wang, Yizhi Song, Xiaoyi Tang, Dunzhu Dawa, Bo Jiang, Dayi Zhang. Application of magnetic-nanoparticle functionalized whole-cell biosensor array for bioavailability and ecotoxicity estimation at urban contaminated sites. Science of The Total Environment 2023, 896 , 165292.
    3. Odafe Ejenavi, Tingting Teng, Wenxin Huang, Xinzi Wang, Wenjing Zhang, Dayi Zhang. Online detection of alkanes by a biological-phase microextraction and biosensing (BPME-BS) device. Journal of Hazardous Materials 2023, 452 , 131316.
    4. Shuang Wu, Hanbing Li, Dayi Zhang, Hao Zhang. Simultaneous determination of heavy metal concentrations and toxicities by diffusive gradient in thin films containing Acinetobacter whole-cell bioreporters (Bio-DGT). Environmental Pollution 2023, 320 , 121050.
    5. Jian Zhang, Qingxiao Pang, Qi Wang, Qingsheng Qi, Qian Wang. Modular tuning engineering and versatile applications of genetically encoded biosensors. Critical Reviews in Biotechnology 2022, 42 (7) , 1010-1027.
    6. Claudia F. Moratti, Colin Scott, Nicholas V. Coleman. Synthetic Biology Approaches to Hydrocarbon Biosensors: A Review. Frontiers in Bioengineering and Biotechnology 2022, 9
    7. Jack Xiaoyu Chen, Harrison Steel, Yin-Hu Wu, Yun Wang, Jiabao Xu, Cordelia P. N. Rampley, Ian P. Thompson, Antonis Papachristodoulou, Wei E. Huang, . Development of Aspirin-Inducible Biosensors in Escherichia coli and SimCells. Applied and Environmental Microbiology 2019, 85 (6)
    8. Javier F. Juárez, Begoña Lecube-Azpeitia, Stuart L. Brown, Christopher D. Johnston, George M. Church. Biosensor libraries harness large classes of binding domains for construction of allosteric transcriptional regulators. Nature Communications 2018, 9 (1)
    9. Yingjie Zhou, Johan Memelink, Huub J. M. Linthorst. An E. coli biosensor for screening of cDNA libraries for isochorismate pyruvate lyase-encoding cDNAs. Molecular Genetics and Genomics 2018, 293 (5) , 1181-1190.
    10. Yujiao Sun, Xiaohui Zhao, Dayi Zhang, Aizhong Ding, Cheng Chen, Wei E. Huang, Huichun Zhang. New naphthalene whole-cell bioreporter for measuring and assessing naphthalene in polycyclic aromatic hydrocarbons contaminated site. Chemosphere 2017, 186 , 510-518.
    11. Bo Jiang, Guanghe Li, Yi Xing, Dayi Zhang, Jianli Jia, Zhisong Cui, Xiao Luan, Hui Tang. A whole-cell bioreporter assay for quantitative genotoxicity evaluation of environmental samples. Chemosphere 2017, 184 , 384-392.
    12. Xinzi Wang, Xiaohui Zhao, Hanbing Li, Jianli Jia, Yueqiao Liu, Odafe Ejenavi, Aizhong Ding, Yujiao Sun, Dayi Zhang. Separating and characterizing functional alkane degraders from crude-oil-contaminated sites via magnetic nanoparticle-mediated isolation. Research in Microbiology 2016, 167 (9-10) , 731-744.
    13. Jianli Jia, Hanbing Li, Shuang Zong, Bo Jiang, Guanghe Li, Odafe Ejenavi, Jingrong Zhu, Dayi Zhang. Magnet bioreporter device for ecological toxicity assessment on heavy metal contamination of coal cinder sites. Sensors and Actuators B: Chemical 2016, 222 , 290-299.
    14. Ali Adnan Al-Anizi, Maria Theresa Hellyer, Dayi Zhang. Toxicity assessment and modelling of Moringa oleifera seeds in water purification by whole cell bioreporter. Water Research 2014, 56 , 77-87.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    You’ve supercharged your research process with ACS and Mendeley!

    STEP 1:
    Click to create an ACS ID

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Your Mendeley pairing has expired. Please reconnect