Improving the Performance of a Quadrupole Time-of-Flight Instrument for Macromolecular Mass SpectrometryClick to copy article linkArticle link copied!
- Robert H. H. van den HeuvelRobert H. H. van den HeuvelDepartment of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands, Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands and MS Horizons, 34 Lea Road, Heaton Moor, Stockport, SK4 4JU, United KingdomMore by Robert H. H. van den Heuvel
- Esther van DuijnEsther van DuijnDepartment of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands, Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands and MS Horizons, 34 Lea Road, Heaton Moor, Stockport, SK4 4JU, United KingdomMore by Esther van Duijn
- Hortense MazonHortense MazonDepartment of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands, Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands and MS Horizons, 34 Lea Road, Heaton Moor, Stockport, SK4 4JU, United KingdomMore by Hortense Mazon
- Silvia A. SynowskySilvia A. SynowskyDepartment of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands, Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands and MS Horizons, 34 Lea Road, Heaton Moor, Stockport, SK4 4JU, United KingdomMore by Silvia A. Synowsky
- Kristina LorenzenKristina LorenzenDepartment of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands, Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands and MS Horizons, 34 Lea Road, Heaton Moor, Stockport, SK4 4JU, United KingdomMore by Kristina Lorenzen
- Cees VersluisCees VersluisDepartment of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands, Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands and MS Horizons, 34 Lea Road, Heaton Moor, Stockport, SK4 4JU, United KingdomMore by Cees Versluis
- Stan J. J. BrounsStan J. J. BrounsDepartment of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands, Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands and MS Horizons, 34 Lea Road, Heaton Moor, Stockport, SK4 4JU, United KingdomMore by Stan J. J. Brouns
- Dave LangridgeDave LangridgeDepartment of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands, Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands and MS Horizons, 34 Lea Road, Heaton Moor, Stockport, SK4 4JU, United KingdomMore by Dave Langridge
- John van der OostJohn van der OostDepartment of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands, Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands and MS Horizons, 34 Lea Road, Heaton Moor, Stockport, SK4 4JU, United KingdomMore by John van der Oost
- John HoyesJohn HoyesDepartment of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands, Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands and MS Horizons, 34 Lea Road, Heaton Moor, Stockport, SK4 4JU, United KingdomMore by John Hoyes
- Albert J. R. Heck*Albert J. R. HeckCorresponding author. Phone: +31302536797. Fax: +31302518219. E-mail: [email protected].Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands, Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands and MS Horizons, 34 Lea Road, Heaton Moor, Stockport, SK4 4JU, United KingdomMore by Albert J. R. Heck
Abstract
We modified and optimized a first generation quadrupole time-of-flight (Q-TOF) 1 to perform tandem mass spectrometry on macromolecular protein complexes. The modified instrument allows isolation and subsequent dissociation of high-mass protein complexes through collisions with argon molecules. The modifications of the Q-TOF 1 include the introduction of (1) a flow-restricting sleeve around the first hexapole ion bridge, (2) a low-frequency ion-selecting quadrupole, (3) a high-pressure hexapole collision cell, (4) high-transmission grids in the multicomponent ion lenses, and (5) a low repetition rate pusher. Using these modifications, we demonstrate the experimental isolation of ions up to 12 800 mass-to-charge units and detection of product ions up to 38 150 Da, enabling the investigation of the gas-phase stability, protein complex topology, and quaternary structure of protein complexes. Some of the data reveal a so-far unprecedented new mechanism in gas-phase dissociation of protein oligomers whereby a tetramer complex dissociates into two dimers. These data add to the current debate whether gas-phase structures of protein complexes do retain some of the structural features of the corresponding species in solution. The presented low-cost modifications on a Q-TOF 1 instrument are of interest to everyone working in the fields of macromolecular mass spectrometry and more generic structural biology.
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