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    Microfluidic Integration of Parallel Solid-Phase Liquid Chromatography
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    † Centre for High-Throughput Biology, University of British Columbia, Vancouver BC, Canada
    ‡ Department of Electrical and Computer Engineering, University of British Columbia, Vancouver BC, Canada
    § Department of Chemical and Biological Engineering, University of British Columbia, Vancouver BC, Canada
    # Department of Physics and Astronomy, University of British Columbia, Vancouver BC, Canada
    *E-mail: [email protected]. Fax: +1 604-822-2114.
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    Analytical Chemistry

    Cite this: Anal. Chem. 2013, 85, 5, 2999–3005
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    https://doi.org/10.1021/ac400163u
    Published February 5, 2013
    Copyright © 2013 American Chemical Society
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    Abstract

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    We report the development of a fully integrated microfluidic chromatography system based on a recently developed column geometry that allows for robust packing of high-performance separation columns in poly(dimethylsiloxane) microfluidic devices having integrated valves made by multilayer soft lithography (MSL). The combination of parallel high-performance separation columns and on-chip plumbing was used to achieve a fully integrated system for on-chip chromatography, including all steps of automated sample loading, programmable gradient generation, separation, fluorescent detection, and sample recovery. We demonstrate this system in the separation of fluorescently labeled DNA and parallel purification of reverse transcription polymerase chain reaction (RT-PCR) amplified variable regions of mouse immunoglobulin genes using a strong anion exchange (AEX) resin. Parallel sample recovery in an immiscible oil stream offers the advantage of low sample dilution and high recovery rates. The ability to perform nucleic acid size selection and recovery on subnanogram samples of DNA holds promise for on-chip genomics applications including sequencing library preparation, cloning, and sample fractionation for diagnostics.

    Copyright © 2013 American Chemical Society

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    Analytical Chemistry

    Cite this: Anal. Chem. 2013, 85, 5, 2999–3005
    Click to copy citationCitation copied!
    https://doi.org/10.1021/ac400163u
    Published February 5, 2013
    Copyright © 2013 American Chemical Society
    Request reuse permissions

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