ACS Publications. Most Trusted. Most Cited. Most Read
Optimization of a Single Substrate-Based Fluorometric Assay for Glucose and Lactate Measurement to Assess Preimplantation Single Embryo Quality and Blood in Obese Mouse and Clinical Human Samples
My Activity
    Article

    Optimization of a Single Substrate-Based Fluorometric Assay for Glucose and Lactate Measurement to Assess Preimplantation Single Embryo Quality and Blood in Obese Mouse and Clinical Human Samples
    Click to copy article linkArticle link copied!

    • Seema Thapa
      Seema Thapa
      Department of Biomedical Engineering, School of Medicine, Keimyung University, 1095 Dalgubeol-daero, Dalseo-gu, Daegu 42601, Republic of Korea
      More by Seema Thapa
    • Yun Seok Heo*
      Yun Seok Heo
      Department of Biomedical Engineering, School of Medicine, Keimyung University, 1095 Dalgubeol-daero, Dalseo-gu, Daegu 42601, Republic of Korea
      *Email: [email protected]
      More by Yun Seok Heo
    Other Access OptionsSupporting Information (4)

    Analytical Chemistry

    Cite this: Anal. Chem. 2022, 94, 46, 16171–16179
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.analchem.2c03602
    Published November 9, 2022
    Copyright © 2022 American Chemical Society

    Abstract

    Click to copy section linkSection link copied!
    Abstract Image

    A fluorometric assay for single substrate-based glucose and lactate measurements was demonstrated by adopting an already established protocol for glucose and by optimizing pH, enzyme concentration, substrate concentration, and types of buffers for lactate. Linear calibration curves for glucose and lactate concentrations from 1 to 100 μM were obtained with correlation coefficients (R2) of 0.94 and 0.98, respectively. First, with the optimized protocol, single embryo quality was successfully evaluated. Three different initial stages of embryos (n = 58) were cultured for 24 h, and glycolytic activities were calculated by measuring amounts of glucose consumption and lactate production. Results showed that embryos cultured at a later stage had lower glycolytic activities, implying more developmental activities. Second, glucose and lactate concentrations in blood plasma of diet-induced obese (DIO) mice were measured. Levels of both glucose and lactate in DIO mice were higher than those in normal mice by 2.15 and 3.8 mmol/L, respectively (both p < 0.001). Finally, clinical serum samples were analyzed and categorized into three groups based on their measured glucose concentrations: normal (4.73 ± 0.29 mmol/L), prediabetic (6.49 ± 0.13 mmol/L), and diabetic (11.34 ± 1.36 mmol/L) (p < 0.05). Collectively, this developed technique can be used to select a high-quality embryo for transfer as well as to measure glucose and lactate levels in other biological samples.

    Copyright © 2022 American Chemical Society

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.

    Recommended

    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. Add or change your institution or let them know you’d like them to include access.

    Supporting Information

    Click to copy section linkSection link copied!

    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.analchem.2c03602.

    • Supplementary methodologies, fluorescence analysis, ion compositions, effect of light exposure on fluorescence assay, fluorescence intensities of glucose assay, comparison of other fluorescence methods for the determination of glucose and lactate, measurement ranges of commercially available glucose sensors and lactate sensors, modified KSOM media compositions, metabolic and glycolytic activity analysis of a single embryo, analytical performance in mice blood plasma samples, comparison between lactate concentration measured using a commercial kit and the current protocol, and additional calculations (PDF)

    • Movie S1: 24-h video of morula to expanded blastocyst (MP4)

    • Movie S2: 24-h video of early to hatched blastocyst (MP4)

    • Movie S3: 24-h video of expanded to hatching blastocyst (MP4)

    Terms & Conditions

    Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses). Permission may be obtained from ACS for other uses through requests via the RightsLink permission system: http://pubs.acs.org/page/copyright/permissions.html.

    Cited By

    Click to copy section linkSection link copied!
    Citation Statements
    Explore this article's citation statements on scite.ai

    This article is cited by 1 publications.

    1. Ming Cai, Shuyao Li, Keren Cai, Xinlin Du, Jia Han, Jingyun Hu. Empowering mitochondrial metabolism: Exploring L-lactate supplementation as a promising therapeutic approach for metabolic syndrome. Metabolism 2024, 152 , 155787. https://doi.org/10.1016/j.metabol.2024.155787

    Analytical Chemistry

    Cite this: Anal. Chem. 2022, 94, 46, 16171–16179
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.analchem.2c03602
    Published November 9, 2022
    Copyright © 2022 American Chemical Society

    Article Views

    626

    Altmetric

    -

    Citations

    Learn about these metrics

    Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.

    Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.

    The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated.