Quantitative Investigation of Terbinafine Hydrochloride Absorption into a Living Skin Equivalent Model by MALDI-MSI
- Cristina RussoCristina RussoCentre for Mass Spectrometry Imaging, Biomolecular Research Centre, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, U.K.More by Cristina Russo,
- Neil BrickelbankNeil BrickelbankCentre for Mass Spectrometry Imaging, Biomolecular Research Centre, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, U.K.More by Neil Brickelbank,
- Catherine DuckettCatherine DuckettCentre for Mass Spectrometry Imaging, Biomolecular Research Centre, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, U.K.More by Catherine Duckett,
- Steve MellorSteve MellorCroda International Plc, Cowick Hall, Snaith, Goole, East Yorkshire DN14 9AA, U.K.More by Steve Mellor,
- Stephen RumbelowStephen RumbelowCroda Inc., 315 Cherry Lane New Castle, Delaware 19720, United StatesMore by Stephen Rumbelow, and
- Malcolm R. Clench*Malcolm R. Clench*E-mail: [email protected]Centre for Mass Spectrometry Imaging, Biomolecular Research Centre, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, U.K.More by Malcolm R. Clench
Abstract
The combination of microspotting of analytical and internal standards, matrix sublimation, and recently developed software for quantitative mass spectrometry imaging has been used to develop a high-resolution method for the determination of terbinafine hydrochloride in the epidermal region of a full thickness living skin equivalent model. A quantitative assessment of the effect of the addition of the penetration enhancer (dimethyl isosorbide (DMI)) to the delivery vehicle has also been performed, and data have been compared to those obtained from LC–MS/MS measurements of homogenates of isolated epidermal tissue. At 10% DMI, the levels of signal detected for the drug in the epidermis were 0.20 ± 0.072 mg/g tissue for QMSI and 0.28 ± 0.040 mg/g tissue for LC–MS/MS at 50% DMI 0.69 ± 0.23 mg/g tissue for QMSI and 0.66 ± 0.057 mg/g tissue for LC–MS/MS. Comparison of means and standard deviations indicates no significant difference between the values obtained by the two methods.
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