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Download Hi-Res ImageDownload to MS-PowerPointCite This:Anal. Chem. 2020, 92, 2, 1674-1679
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Experimental Comparison in Sensing Breast Cancer Mutations by Signal ON and Signal OFF Paper-Based Electroanalytical Strips

  • Stefano Cinti*
    Stefano Cinti
    Department of Pharmacy, University of Naples “Federico II”, Via D. Montesano 49, 80131 Naples, Italy
    *Stefano Cinti, Email: [email protected]
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    Orcidhttp://orcid.org/0000-0002-8274-7452
  • , 
  • Giulia Cinotti
    Giulia Cinotti
    Department of Chemical Science and Technologies, University of Rome “Tor Vergata”, Via della Ricerca Scientifica 1, 00133 Rome, Italy
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  • , 
  • Claudio Parolo
    Claudio Parolo
    Nanobioelectronics & Biosensors Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BIST, Campus UAB, Bellaterra, 08193 Barcelona, Spain
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    Orcidhttp://orcid.org/0000-0001-9481-4408
  • , 
  • Emily P. Nguyen
    Emily P. Nguyen
    Nanobioelectronics & Biosensors Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BIST, Campus UAB, Bellaterra, 08193 Barcelona, Spain
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  • , 
  • Veronica Caratelli
    Veronica Caratelli
    Department of Chemical Science and Technologies, University of Rome “Tor Vergata”, Via della Ricerca Scientifica 1, 00133 Rome, Italy
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  • , 
  • Danila Moscone
    Danila Moscone
    Department of Chemical Science and Technologies, University of Rome “Tor Vergata”, Via della Ricerca Scientifica 1, 00133 Rome, Italy
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  • Fabiana Arduini
    Fabiana Arduini
    Department of Chemical Science and Technologies, University of Rome “Tor Vergata”, Via della Ricerca Scientifica 1, 00133 Rome, Italy
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  • , and 
  • Arben Merkoci*
    Arben Merkoci
    Nanobioelectronics & Biosensors Group, Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BIST, Campus UAB, Bellaterra, 08193 Barcelona, Spain
    ICREA, Institució Catalana de Recerca i Estudis Avançats, Pg. Lluís Companys 23, 08010 Barcelona, Spain
    *Arben Merkoci, Email: [email protected]
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    Orcidhttp://orcid.org/0000-0003-2486-8085
Cite this: Anal. Chem. 2020, 92, 2, 1674–1679
Publication Date (Web):December 26, 2019
https://doi.org/10.1021/acs.analchem.9b02560
Copyright © 2019 American Chemical Society
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    Abstract

    Abstract Image

    The development of paper-based electroanalytical strips as powerful diagnostic tools has gained a lot of attention within the sensor community. In particular, the detection of nucleic acids in complex matrices represents a trending topic, especially when focused toward the development of emerging technologies, such as liquid biopsy. DNA-based biosensors have been largely applied in this direction, and currently, there are two main approaches based on target/probe hybridization reported in the literature, namely Signal ON and Signal OFF. In this technical note, the two approaches are evaluated in combination with paper-based electrodes, using a single strand DNA relative to H1047R (A3140G) missense mutation in exon 20 in breast cancer as the model target. A detailed comparison among the analytical performances, detection protocol, and cost associated with the two systems is provided, highlighting the advantages and drawbacks depending on the application. The present work is aimed to a wide audience, particularly for those in the field of point-of-care, and it is intended to provide the know-how to manage with the design and development stages, and to optimize the platform for the sensing of nucleic acids using a paper-based detection method.

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    • Details on the differential pulse voltammograms obtained in the presence and in the absence of ferricyanide as the electrochemical mediator, the evaluation of the electrochemical response at different densities of probes immobilized in the signal ON architecture when different washing steps are performed, and selectivity studies of the two approaches (PDF)

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