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Electrokinetically Driven Exosome Separation and Concentration Using Dielectrophoretic-Enhanced PDMS-Based Microfluidics

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Download Hi-Res ImageDownload to MS-PowerPointCite This:Anal. Chem. 2019, 91, 23, 14975-14982
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    Electrokinetically Driven Exosome Separation and Concentration Using Dielectrophoretic-Enhanced PDMS-Based Microfluidics
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    • Sergio Ayala-Mar
      Sergio Ayala-Mar
      Tecnologico de Monterrey, School of Engineering and Science, Av. Eugenio Garza Sada 2501, Monterrey, NL 64849, Mexico
      More by Sergio Ayala-Mar
    • Victor H. Perez-Gonzalez
      Victor H. Perez-Gonzalez
      Tecnologico de Monterrey, School of Engineering and Science, Av. Eugenio Garza Sada 2501, Monterrey, NL 64849, Mexico
      More by Victor H. Perez-Gonzalez
    • Marco A. Mata-Gómez
      Marco A. Mata-Gómez
      Tecnologico de Monterrey, School of Engineering and Science, Av. Eugenio Garza Sada 2501, Monterrey, NL 64849, Mexico
      More by Marco A. Mata-Gómez
      Orcidhttp://orcid.org/0000-0002-3457-7030
    • Roberto C. Gallo-Villanueva*
      Roberto C. Gallo-Villanueva
      Tecnologico de Monterrey, School of Engineering and Science, Av. Eugenio Garza Sada 2501, Monterrey, NL 64849, Mexico
      *E-mail: [email protected]. Tel.: +52(81)83582000.
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    • José González-Valdez*
      José González-Valdez
      Tecnologico de Monterrey, School of Engineering and Science, Av. Eugenio Garza Sada 2501, Monterrey, NL 64849, Mexico
      *E-mail: [email protected]. Tel.: +52(81)83582000.
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      Orcidhttp://orcid.org/0000-0001-6734-8245
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    Analytical Chemistry

    Cite this: Anal. Chem. 2019, 91, 23, 14975–14982
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    https://doi.org/10.1021/acs.analchem.9b03448
    Published November 18, 2019
    Copyright © 2019 American Chemical Society
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    Abstract

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    Exosomes are a specific subpopulation of extracellular vesicles that have gained interest because of their many potential biomedical applications. However, exosome isolation and characterization are the first steps toward designing novel applications. This work presents a direct current–insulator-based dielectrophoretic (DC-iDEP) approach to simultaneously capture and separate exosomes by size. To do so, a microdevice consisting of a channel with two electrically insulating post sections was designed. Each section was tailored to generate different nonuniform spatial distributions of the electric field and, therefore, different dielectrophoretic forces acting on exosomes suspended in solution. Side channels were placed adjacent to each section to allow sample recovery. By applying an electric potential difference of 2000 V across the length of the main channel, dielectrophoretic size-based separation of exosomes was observed in the device. Analysis of particle size in each recovered fraction served to assess exosome separation efficiency. These findings show that iDEP can represent a first step toward designing a high-throughput, fast, and robust microdevice capable of capturing and discriminating different subpopulations of exosomes based on their size.

    Copyright © 2019 American Chemical Society

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.analchem.9b03448.

    • Theory of electrokinetics; partial experimental methods used in this work; schematic representation of the microfluidic device; and numerical simulations showing the distribution and magnitude of ∇(E⃗ · E⃗) in both sections, obtained by applying DC voltages of 1500 and 2000 V (PDF)

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    Cite this: Anal. Chem. 2019, 91, 23, 14975–14982
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    https://doi.org/10.1021/acs.analchem.9b03448
    Published November 18, 2019
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