Mineralization of Layer-by-Layer Ultrathin Films Containing Microfluidic-Produced Hydroxyapatite Nanorods
- Ramón RialRamón RialSoft Matter and Molecular Biophysics Group, Department of Applied Physics, University of Santiago de Compostela, 15782, Santiago de Compostela, SpainMore by Ramón Rial,
- Rui R. CostaRui R. Costa3B’s Research Group, I3Bs − Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, PortugalICVS/3B’s, PT Government Associated Laboratory, Braga/Guimarães, PortugalMore by Rui R. Costa,
- Rui L. ReisRui L. Reis3B’s Research Group, I3Bs − Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, PortugalICVS/3B’s, PT Government Associated Laboratory, Braga/Guimarães, PortugalThe Discoveries Centre for Regenerative and Precision Medicine, Headquarters at University of Minho, Avepark, 4805-017 Barco, Guimarães, PortugalMore by Rui L. Reis,
- Zhen LiuZhen LiuDepartment of Physics and Engineering, Frostburg State University, Frostburg, Maryland 21532, United StatesMore by Zhen Liu,
- Iva Pashkuleva*Iva Pashkuleva*(I.P.) E-mail: [email protected]3B’s Research Group, I3Bs − Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, PortugalICVS/3B’s, PT Government Associated Laboratory, Braga/Guimarães, PortugalMore by Iva Pashkuleva, and
- Juan M. Ruso*Juan M. Ruso*(J.M.R.) E-mail: [email protected]Soft Matter and Molecular Biophysics Group, Department of Applied Physics, University of Santiago de Compostela, 15782, Santiago de Compostela, SpainMore by Juan M. Ruso
Abstract
We describe the assembly of layer-by-layer (LbL) ultrathin films containing bioactive hydroxyapatite (HAp) rod-shaped nanoparticles with mineralizing capacity. Monodisperse 96 nm long and 9 nm wide HAp nanorods with a surface charge of −14 mV were produced with a microfluidic system. The negatively charged HAp nanorods were assembled with the polycation poly-l-lysine (PLL) in LbL fashion. The successful deposition of alternating layers was confirmed by a quartz-crystal microbalance with dissipation monitoring. The Voigt-based viscoelastic model demonstrated steady film growth where three PLL/HAp bilayers reached a thickness of 70 nm. The bioactivity of [PLL/HAp]3 was evaluated in vitro by following the formation of a mineralized hydroxyapatite layer in simulated body fluid (SBF). X-ray diffraction, energy-dispersive X-ray spectroscopy, and scanning electron microscopy (SEM) demonstrated formation of a crystalline hydroxyapatite layer and complete surface coverage within 7 days. SaOs-2 osteoblasts-like cells attached to the mineralized surfaces and developed longer filopodia extensions when compared to nonmineralized samples. Our results showed that [PLL/HAp]3 films are feasible osteoconductive coatings applicable to orthopedic implants and fixation devices.
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