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Investigating the Thermodynamics Underlying Monosaccharide-Mediated Collagen Polymerization for Materials Design
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    Investigating the Thermodynamics Underlying Monosaccharide-Mediated Collagen Polymerization for Materials Design
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    • Cassandra L. Martin
      Cassandra L. Martin
      Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts 02115, United States
    • Michael R. Bergman
      Michael R. Bergman
      Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts 02115, United States
    • Patrick A. Sullivan
      Patrick A. Sullivan
      Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts 02115, United States
    • Leila F. Deravi*
      Leila F. Deravi
      Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts 02115, United States
      *Email: [email protected]
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    Chemistry of Materials

    Cite this: Chem. Mater. 2022, 34, 7, 3099–3108
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    https://doi.org/10.1021/acs.chemmater.1c04153
    Published March 24, 2022
    Copyright © 2022 American Chemical Society

    Abstract

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    The use and incorporation of type I collagen (COL) in biomaterials and regenerative medicine have remained challenging due to COL’s ability to spontaneously polymerize at physiological pH in vitro. Previous work has shown that the addition of monosaccharides can delay COL polymerization and increase its solubility under neutral conditions by three orders of magnitude─two features that enable structure retention and integration into pre-existing fibrous networks. We expand on these findings and describe the thermodynamic effects of these monosaccharides on the growth phase of COL polymerization. We derive van’t Hoff plots for each experimental condition and use these data to support an indirect mechanism for delayed COL assembly profiles based on solvent ordering. We observe that the presence of monosaccharides in a COL solution neither altered nor permanently inhibited the formation of COL fibrils. Finally, we demonstrate the utility of our findings through a proof-of-concept study which showed how the presence of these monosaccharides aided in the delivery of a high (2 mg/mL) concentration of neutralized, monomeric COL into simple patterns without disrupting COL structure formation. Our findings support the application of entropy-regulating systems such as monosaccharides in manipulating the dynamics of some self-assembling proteins to aid in biomaterials design.

    Copyright © 2022 American Chemical Society

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    Supporting Information

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    The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.chemmater.1c04153.

    • Analysis of the rate order of COL polymerization; turbidimetric assays at varying monosaccharide concentrations and temperatures; COL controls in the absence of monosaccharides; SDS-PAGE gel results of the COL supernatant; DLS results for COL polymerization; effect of D2O on the COL assembly kinetics; and thermal stability analysis of COL fibrils with and without Gal (PDF)

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    This article is cited by 1 publications.

    1. Marcos Cortes-Medina, Andrew R. Bushman, Peter E. Beshay, Jonathan J. Adorno, Miles M. Menyhert, Riley M. Hildebrand, Shashwat S. Agarwal, Alex Avendano, Alicia K. Friedman, Jonathan W. Song. Chondroitin sulfate, dermatan sulfate, and hyaluronic acid differentially modify the biophysical properties of collagen-based hydrogels. Acta Biomaterialia 2024, 174 , 116-126. https://doi.org/10.1016/j.actbio.2023.12.018

    Chemistry of Materials

    Cite this: Chem. Mater. 2022, 34, 7, 3099–3108
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.chemmater.1c04153
    Published March 24, 2022
    Copyright © 2022 American Chemical Society

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