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Common E-Cigarette Flavoring Chemicals Impair Neutrophil Phagocytosis and Oxidative Burst

  • Elise Hickman
    Elise Hickman
    Curriculum in Toxicology & Environmental Medicine  and  Center for Environmental Medicine, Asthma, and Lung Biology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27514, United States
  • Carolina A. Herrera
    Carolina A. Herrera
    Department of Pathology and Laboratory Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27514, United States
  • , and 
  • Ilona Jaspers*
    Ilona Jaspers
    Curriculum in Toxicology & Environmental Medicine  and  Center for Environmental Medicine, Asthma, and Lung Biology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27514, United States
    *E-mail: [email protected]
Cite this: Chem. Res. Toxicol. 2019, 32, 6, 982–985
Publication Date (Web):May 22, 2019
Copyright © 2019 American Chemical Society

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    E-cigarette flavorings have not been thoroughly evaluated for inhalational toxicity. We have shown that the flavoring chemical cinnamaldehyde impairs human neutrophils, macrophages, and natural killer cells. Here we investigated the effects of other common e-liquid flavoring chemicals on phagocytosis and oxidative burst in neutrophils. We demonstrate that cinnamaldehyde and ethyl vanillin dose-dependently decrease oxidative burst and that benzaldehyde and benzaldehyde propylene glycol acetal dose-dependently impair phagocytosis. Isoamyl acetate did not affect either measure of neutrophil function. These data suggest that inhaling aromatic aldehydic flavoring chemicals, such as cinnamaldehyde, benzaldehyde, benzaldehyde propylene glycol acetal, or ethyl vanillin, could impair neutrophil function.

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.chemrestox.9b00171.

    • Overall study demographics; maximum OCR for each flavoring chemical; time to maximum OCR for each flavoring chemical; maximum ECAR for each flavoring chemical; time to maximum ECAR for each flavoring chemical; IC50 values for neutrophil oxidative burst AUC and phagocytosis; neutrophil oxidative burst rate graphs; neutrophil glycolysis during oxidative burst (PDF)

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