Assessing and Reducing the Toxicity of 3D-Printed Parts
- Shirin Mesbah Oskui ,
- Graciel Diamante ,
- Chunyang Liao ,
- Wei Shi ,
- Jay Gan ,
- Daniel Schlenk , and
- William H. Grover
Abstract

3D printing is gaining popularity by providing a tool for fast, cost-effective, and highly customizable fabrication. However, little is known about the toxicity of 3D-printed objects. In this work, we assess the toxicity of printed parts from two main classes of commercial 3D printers, fused deposition modeling and stereolithography. We assessed the toxicity of these 3D-printed parts using zebrafish (Danio rerio), a widely used model organism in aquatic toxicology. Zebrafish embryos were exposed to 3D-printed parts and monitored for rates of survival, hatching, and developmental abnormalities. We found that parts from both types of printers were measurably toxic to zebrafish embryos, with STL-printed parts significantly more toxic than FDM-printed parts. We also developed a simple post-printing treatment (exposure to ultraviolet light) that largely mitigates the toxicity of the STL-printed parts. Our results call attention to the need for strategies for the safe disposal of 3D-printed parts and printer waste materials.
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- Neelam Bharti, Shailendra Singh. COVID-19: The Use of 3D Printing to Address PPE Shortage during a Pandemic—A Safety Perspective. ACS Chemical Health & Safety 2020, 27 (6) , 335-340. https://doi.org/10.1021/acs.chas.0c00089
- Hyun June Moon, Minhee Ku, Yoon Ho Roh, Hyun Jee Lee, Jaemoon Yang, Ki Wan Bong. Elimination of Unreacted Acrylate Double Bonds in the Polymer Networks of Microparticles Synthesized via Flow Lithography. Langmuir 2020, 36 (9) , 2271-2277. https://doi.org/10.1021/acs.langmuir.9b02737
- Vincent S. D. Voet, Tobias Strating, Geraldine H. M. Schnelting, Peter Dijkstra, Martin Tietema, Jin Xu, Albert J. J. Woortman, Katja Loos, Jan Jager, and Rudy Folkersma . Biobased Acrylate Photocurable Resin Formulation for Stereolithography 3D Printing. ACS Omega 2018, 3 (2) , 1403-1408. https://doi.org/10.1021/acsomega.7b01648
- Marcia de Almeida Monteiro Melo Ferraz, Heiko H. W. Henning, Pedro Ferreira da Costa, Jos Malda, Séverine Le Gac, Fabrice Bray, Majorie B. M. van Duursen, Jos F. Brouwers, Chris H. A. van de Lest, Ingeborg Bertijn, Lisa Kraneburg, Peter L. A. M. Vos, Tom A. E. Stout, and Barend M. Gadella . Potential Health and Environmental Risks of Three-Dimensional Engineered Polymers. Environmental Science & Technology Letters 2018, 5 (2) , 80-85. https://doi.org/10.1021/acs.estlett.7b00495
- Allison M. Pekkanen, Callie Zawaski, André T. Stevenson, Jr., Ross Dickerman, Abby R. Whittington, Christopher B. Williams, and Timothy E. Long . Poly(ether ester) Ionomers as Water-Soluble Polymers for Material Extrusion Additive Manufacturing Processes. ACS Applied Materials & Interfaces 2017, 9 (14) , 12324-12331. https://doi.org/10.1021/acsami.7b01777
- Niall P. Macdonald, Joan M. Cabot, Petr Smejkal, Rosanne M. Guijt, Brett Paull, and Michael C. Breadmore . Comparing Microfluidic Performance of Three-Dimensional (3D) Printing Platforms. Analytical Chemistry 2017, 89 (7) , 3858-3866. https://doi.org/10.1021/acs.analchem.7b00136
- Ligang Hu and Guibin Jiang . 3D Printing Techniques in Environmental Science and Engineering Will Bring New Innovation. Environmental Science & Technology 2017, 51 (7) , 3597-3599. https://doi.org/10.1021/acs.est.7b00302
- Karen Smiar and J. D. Mendez . Creating and Using Interactive, 3D-Printed Models to Improve Student Comprehension of the Bohr Model of the Atom, Bond Polarity, and Hybridization. Journal of Chemical Education 2016, 93 (9) , 1591-1594. https://doi.org/10.1021/acs.jchemed.6b00297
- Charalampos Tzivelekis, Matthew P Selby, Albert Batet, Hojjat Madadi, Kenny Dalgarno. Microfluidic chip fabrication and performance analysis of 3D printed material for use in microfluidic nucleic acid amplification applications. Journal of Micromechanics and Microengineering 2021, 31 (3) , 035005. https://doi.org/10.1088/1361-6439/abd9a9
- Ajith Damodaran, M. Sugavaneswaran, Larry Lessard. An overview of additive manufacturing technologies for musical wind instruments. SN Applied Sciences 2021, 3 (2) https://doi.org/10.1007/s42452-021-04170-x
- Mohamad Amin Jumat, Pascale Chevallier, Diego Mantovani, Francesco Copes, Saiful Izwan Abd Razak, Syafiqah Saidin. Three-dimensional printed biodegradable poly(l-lactic acid)/(poly(d-lactic acid) scaffold as an intervention of biomedical substitute. Polymer-Plastics Technology and Materials 2021, , 1-11. https://doi.org/10.1080/25740881.2021.1876879
- Reymark D. Maalihan, Qiyi Chen, Joseph Rey H. Sta. Agueda, Bryan B. Pajarito, Hiroshi Tamura, Rigoberto C. Advincula. On the Use of Surfactant‐Complexed Chitosan for Toughening 3D Printed Polymethacrylate Composites. Macromolecular Materials and Engineering 2021, 306 (1) , 2000448. https://doi.org/10.1002/mame.202000448
- Aluri Manoj, Monami Bhuyan, Swarup Raj Banik, Mamilla Ravi Sankar. Review on particle emissions during fused deposition modeling of acrylonitrile butadiene styrene and polylactic acid polymers. Materials Today: Proceedings 2021, https://doi.org/10.1016/j.matpr.2020.11.521
- Jing Wee Koo, Jia Shin Ho, Jia An, Yi Zhang, Chee Kai Chua, Tzyy Haur Chong. A review on spacers and membranes: Conventional or hybrid additive manufacturing?. Water Research 2021, 188 , 116497. https://doi.org/10.1016/j.watres.2020.116497
- Anda Barkane, Oskars Platnieks, Maksims Jurinovs, Sergejs Gaidukovs. Thermal stability of UV-cured vegetable oil epoxidized acrylate-based polymer system for 3D printing application. Polymer Degradation and Stability 2020, 181 , 109347. https://doi.org/10.1016/j.polymdegradstab.2020.109347
- Dingyi Wang, Junpeng Zhang, Qian Liu, Bolei Chen, Yong Liang, Ligang Hu, Guibin Jiang. 3D Printing Challenges in Enabling Rapid Response to Public Health Emergencies. The Innovation 2020, 1 (3) , 100056. https://doi.org/10.1016/j.xinn.2020.100056
- Zhi Zhao, Xiaoxiao Tian, Xiaoyan Song. Engineering materials with light: recent progress in digital light processing based 3D printing. Journal of Materials Chemistry C 2020, 8 (40) , 13896-13917. https://doi.org/10.1039/D0TC03548C
- Theona Șucu, Michael P. Shaver. Inherently degradable cross-linked polyesters and polycarbonates: resins to be cheerful. Polymer Chemistry 2020, 11 (40) , 6397-6412. https://doi.org/10.1039/D0PY01226B
- Xiaoyan Xu, Atheer Awad, Pamela Robles-Martinez, Simon Gaisford, Alvaro Goyanes, Abdul W. Basit. Vat photopolymerization 3D printing for advanced drug delivery and medical device applications. Journal of Controlled Release 2020, https://doi.org/10.1016/j.jconrel.2020.10.008
- Adam Tepperman, David Jiao Zheng, Maria Abou Taka, Angela Vrieze, Austin Le Lam, Bryan Heit. Customizable live-cell imaging chambers for multimodal and multiplex fluorescence microscopy. Biochemistry and Cell Biology 2020, 98 (5) , 612-623. https://doi.org/10.1139/bcb-2020-0064
- Gustavo González, Désirée Baruffaldi, Cinzia Martinengo, Angelo Angelini, Annalisa Chiappone, Ignazio Roppolo, Candido Fabrizio Pirri, Francesca Frascella. Materials Testing for the Development of Biocompatible Devices through Vat-Polymerization 3D Printing. Nanomaterials 2020, 10 (9) , 1788. https://doi.org/10.3390/nano10091788
- Ignacio Muro-Fraguas, Ana Sainz-García, Paula Fernández Gómez, María López, Rodolfo Múgica-Vidal, Elisa Sainz-García, Paula Toledano, Yolanda Sáenz, Mercedes López, Montserrat González-Raurich, Miguel Prieto, Avelino Alvarez-Ordóñez, Ana González-Marcos, Fernando Alba-Elías. Atmospheric pressure cold plasma anti-biofilm coatings for 3D printed food tools. Innovative Food Science & Emerging Technologies 2020, 64 , 102404. https://doi.org/10.1016/j.ifset.2020.102404
- Marline Kirsch, Annabelle-Christin Herder, Cécile Boudot, Andreas Karau, Jessica Rach, Wiebke Handke, Axel Seltsam, Thomas Scheper, Antonina Lavrentieva. Xeno-Free In Vitro Cultivation and Osteogenic Differentiation of hAD-MSCs on Resorbable 3D Printed RESOMER®. Materials 2020, 13 (15) , 3399. https://doi.org/10.3390/ma13153399
- Ranvijay Kumar, Rupinder Singh, I. P. S. Ahuja, M. S. J. Hashmi. Processing techniques of polymeric materials and their reinforced composites. Advances in Materials and Processing Technologies 2020, 6 (3) , 591-607. https://doi.org/10.1080/2374068X.2020.1728989
- Seng Han Lim, Wen Jun Tiew, Junying Zhang, Paul Chi-Lui Ho, Nezamoddin N. Kachouie, Lifeng Kang. Geometrical optimisation of a personalised microneedle eye patch for transdermal delivery of anti-wrinkle small peptide. Biofabrication 2020, 12 (3) , 035003. https://doi.org/10.1088/1758-5090/ab6d37
- Kati Piironen, Markus Haapala, Virpi Talman, Päivi Järvinen, Tiina Sikanen. Cell adhesion and proliferation on common 3D printing materials used in stereolithography of microfluidic devices. Lab on a Chip 2020, 20 (13) , 2372-2382. https://doi.org/10.1039/D0LC00114G
- Keaton Nahan, Eric M. Sussman, Berk Oktem, Lester Schultheis, Samanthi Wickramasekara. Screening for extractables in additive-manufactured acrylonitrile butadiene styrene orthopedic cast. Talanta 2020, 212 , 120464. https://doi.org/10.1016/j.talanta.2019.120464
- . Umgang und Gebrauch von additiven Fertigungsverfahren („3D-Druckern“) in Privathaushalten. Bundesgesundheitsblatt - Gesundheitsforschung - Gesundheitsschutz 2020,,, 370-371. https://doi.org/10.1007/s00103-020-03095-x
- Qianqian Wang, Chencheng Ji, Jianzhong Sun, Qian Yao, Jun Liu, Rana Muhammad Yousaf Saeed, Qianqian Zhu. Kinetic thermal behavior of nanocellulose filled polylactic acid filament for fused filament fabrication 3D printing. Journal of Applied Polymer Science 2020, 137 (7) , 48374. https://doi.org/10.1002/app.48374
- Blessy Joseph, Jemy James, Yves Grohens, Nandakumar Kalarikkal, Sabu Thomas. Material aspects during additive manufacturing of nano-cellulose composites. 2020,,, 409-428. https://doi.org/10.1016/B978-0-12-819535-2.00014-4
- . Structure and Properties of Additive Manufactured Polymer Components. 2020,,https://doi.org/
- Brian Crites, Karen Kong, Philip Brisk. Directed Placement for mVLSI Devices. ACM Journal on Emerging Technologies in Computing Systems 2019, 16 (2) , 1-26. https://doi.org/10.1145/3369585
- Mariana T. Farcas, Aleksandr B. Stefaniak, Alycia K. Knepp, Lauren Bowers, William K. Mandler, Michael Kashon, Stephen R. Jackson, Todd A. Stueckle, Jenifer D. Sisler, Sherri A. Friend, Chaolong Qi, Duane R. Hammond, Treye A. Thomas, Joanna Matheson, Vincent Castranova, Yong Qian. Acrylonitrile butadiene styrene (ABS) and polycarbonate (PC) filaments three-dimensional (3-D) printer emissions-induced cell toxicity. Toxicology Letters 2019, 317 , 1-12. https://doi.org/10.1016/j.toxlet.2019.09.013
- Teboho Clement Mokhena, Maya Jacob John, Mokgaotsa Jonas Mochane, Asanda Mtibe, Teboho Simon Motsoeneng, Thabang Hendrica Mokhothu, Cyrus Alushavhiwi Tshifularo. Cellulose Nanocrystals‐Based Composites. 2019,,, 283-306. https://doi.org/10.1002/9781119592129.ch16
- , , . Soil Microenvironment for Bioremediation and Polymer Production. 2019,,https://doi.org/10.1002/9781119592129
- Frank Alifui-Segbaya. Biomedical photopolymers in 3D printing. Rapid Prototyping Journal 2019, 26 (2) , 437-444. https://doi.org/10.1108/RPJ-10-2018-0268
- Andrew C. Weems, Kayla R. Delle Chiaie, Joshua C. Worch, Connor J. Stubbs, Andrew P. Dove. Terpene- and terpenoid-based polymeric resins for stereolithography 3D printing. Polymer Chemistry 2019, 10 (44) , 5959-5966. https://doi.org/10.1039/C9PY00950G
- Paula K. Kuroishi, Kayla R. Delle Chiaie, Andrew P. Dove. Polylactide thermosets using a bis(cyclic diester) crosslinker. European Polymer Journal 2019, 120 , 109192. https://doi.org/10.1016/j.eurpolymj.2019.08.019
- Milanga Walpitagama, Megan Carve, Alon M. Douek, Charlene Trestrail, Yutao Bai, Jan Kaslin, Donald Wlodkowic. Additives migrating from 3D-printed plastic induce developmental toxicity and neuro-behavioural alterations in early life zebrafish (Danio rerio). Aquatic Toxicology 2019, 213 , 105227. https://doi.org/10.1016/j.aquatox.2019.105227
- George Inyila Ogoh, N. Ben Fairweather. The state of the responsible research and innovation programme. Journal of Information, Communication and Ethics in Society 2019, 17 (2) , 145-166. https://doi.org/10.1108/JICES-12-2018-0093
- Matthew Walker, Stuart Humphries. 3D Printing: Applications in evolution and ecology. Ecology and Evolution 2019, 9 (7) , 4289-4301. https://doi.org/10.1002/ece3.5050
- Katie Hardiman. Post-processing Considerations for Biomedical 3D Printing of Polymers. 2019,,, 219-241. https://doi.org/10.1007/978-3-030-24532-0_10
- . Polymer-Based Additive Manufacturing. 2019,,https://doi.org/10.1007/978-3-030-24532-0
- Liliana Liverani, Vincenzo Guarino, Vincenzo La Carrubba, Aldo R. Boccaccini. Porous Biomaterials and Scaffolds for Tissue Engineering. 2019,,, 188-202. https://doi.org/10.1016/B978-0-12-801238-3.99872-6
- . Encyclopedia of Biomedical Engineering. 2019,,https://doi.org/
- Diego Savio Branciforti, Simone Lazzaroni, Chiara Milanese, Marco Castiglioni, Ferdinando Auricchio, Dario Pasini, Daniele Dondi. Visible light 3D printing with epoxidized vegetable oils. Additive Manufacturing 2019, 25 , 317-324. https://doi.org/10.1016/j.addma.2018.11.020
- Sanlin S. Robinson, Cameron A. Aubin, Thomas J. Wallin, Saleh Gharaie, Patricia A. Xu, Kaiyang Wang, Simon N. Dunham, Bobak Mosadegh, Robert F. Shepherd. Stereolithography for Personalized Left Atrial Appendage Occluders. Advanced Materials Technologies 2018, 3 (12) , 1800233. https://doi.org/10.1002/admt.201800233
- Andrew J. Capel, Rowan P. Rimington, Mark P. Lewis, Steven D. R. Christie. 3D printing for chemical, pharmaceutical and biological applications. Nature Reviews Chemistry 2018, 2 (12) , 422-436. https://doi.org/10.1038/s41570-018-0058-y
- Jocelyn E. Behm, Brenna R. Waite, S. Tonia Hsieh, Matthew R. Helmus. Benefits and limitations of three-dimensional printing technology for ecological research. BMC Ecology 2018, 18 (1) https://doi.org/10.1186/s12898-018-0190-z
- Agnieszka Haryńska, Iga Gubanska, Justyna Kucinska-Lipka, Helena Janik. Fabrication and Characterization of Flexible Medical-Grade TPU Filament for Fused Deposition Modeling 3DP Technology. Polymers 2018, 10 (12) , 1304. https://doi.org/10.3390/polym10121304
- Frank Alifui-Segbaya, Jasper Bowman, Alan R. White, Sony Varma, Graham J. Lieschke, Roy George. Toxicological assessment of additively manufactured methacrylates for medical devices in dentistry. Acta Biomaterialia 2018, 78 , 64-77. https://doi.org/10.1016/j.actbio.2018.08.007
- Chantell Farias, Roman Lyman, Cecilia Hemingway, Huong Chau, Anne Mahacek, Evangelia Bouzos, Maryam Mobed-Miremadi. Three-Dimensional (3D) Printed Microneedles for Microencapsulated Cell Extrusion. Bioengineering 2018, 5 (3) , 59. https://doi.org/10.3390/bioengineering5030059
- Blanche C. Ip, Francis Cui, Benjamin T. Wilks, John Murphy, Anubhav Tripathi, Jeffrey R. Morgan. Perfused Organ Cell-Dense Macrotissues Assembled from Prefabricated Living Microtissues. Advanced Biosystems 2018, 2 (8) , 1800076. https://doi.org/10.1002/adbi.201800076
- Rhys Cartlidge, Donald Wlodkowic. Rapid Fabrication of Chip-Based Physiometers for Neurobehavioral Toxicity Assays Using Rotifers Brachionus calyciflorus. Cytometry Part A 2018, 93 (8) , 837-847. https://doi.org/10.1002/cyto.a.23510
- Qianqian Wang, Jianzhong Sun, Qian Yao, Chencheng Ji, Jun Liu, Qianqian Zhu. 3D printing with cellulose materials. Cellulose 2018, 25 (8) , 4275-4301. https://doi.org/10.1007/s10570-018-1888-y
- Atheer Awad, Sarah J. Trenfield, Alvaro Goyanes, Simon Gaisford, Abdul W. Basit. Reshaping drug development using 3D printing. Drug Discovery Today 2018, 23 (8) , 1547-1555. https://doi.org/10.1016/j.drudis.2018.05.025
- Rowan P. Rimington, Andrew J. Capel, Darren J. Player, Richard J. Bibb, Steven D. R. Christie, Mark P. Lewis. Feasibility and Biocompatibility of 3D-Printed Photopolymerized and Laser Sintered Polymers for Neuronal, Myogenic, and Hepatic Cell Types. Macromolecular Bioscience 2018, 18 (7) , 1800113. https://doi.org/10.1002/mabi.201800113
- I. Gümperlein, E. Fischer, G. Dietrich-Gümperlein, S. Karrasch, D. Nowak, R. A. Jörres, R. Schierl. Acute health effects of desktop 3D printing (fused deposition modeling) using acrylonitrile butadiene styrene and polylactic acid materials: An experimental exposure study in human volunteers. Indoor Air 2018, 28 (4) , 611-623. https://doi.org/10.1111/ina.12458
- Liang Gie Huang, Ching Jui Shih, Chia Hung Yeh. Optimal designable function utilized in medical device by 3D printing technique. 2018,,, 1030-1033. https://doi.org/10.1109/ICASI.2018.8394451
- . 2018 IEEE International Conference on Applied System Invention (ICASI). 2018,,https://doi.org/
- Eric Lepowsky, Savas Tasoglu. Emerging Anti-Fouling Methods: Towards Reusability of 3D-Printed Devices for Biomedical Applications. Micromachines 2018, 9 (4) , 196. https://doi.org/10.3390/mi9040196
- Nurul Mohd Fuad, Megan Carve, Jan Kaslin, Donald Wlodkowic. Characterization of 3D-Printed Moulds for Soft Lithography of Millifluidic Devices. Micromachines 2018, 9 (3) , 116. https://doi.org/10.3390/mi9030116
- Megan Carve, Donald Wlodkowic. 3D-Printed Chips: Compatibility of Additive Manufacturing Photopolymeric Substrata with Biological Applications. Micromachines 2018, 9 (2) , 91. https://doi.org/10.3390/mi9020091
- Pamela Robles Martinez, Abdul W. Basit, Simon Gaisford. The History, Developments and Opportunities of Stereolithography. 2018,,, 55-79. https://doi.org/10.1007/978-3-319-90755-0_4
- , . 3D Printing of Pharmaceuticals. 2018,,https://doi.org/10.1007/978-3-319-90755-0
- Munan Li, Alan L. Porter. Facilitating the discovery of relevant studies on risk analysis for three-dimensional printing based on an integrated framework. Scientometrics 2018, 114 (1) , 277-300. https://doi.org/10.1007/s11192-017-2570-0
- Chengchen Rao, Fu Gu, Peng Zhao, Nusrat Sharmin, Haibing Gu, Jianzhong Fu. Capturing PM2.5 Emissions from 3D Printing via Nanofiber-based Air Filter. Scientific Reports 2017, 7 (1) https://doi.org/10.1038/s41598-017-10995-7
- Frank Alifui-Segbaya, Sony Varma, Graham J. Lieschke, Roy George. Biocompatibility of Photopolymers in 3D Printing. 3D Printing and Additive Manufacturing 2017, 4 (4) , 185-191. https://doi.org/10.1089/3dp.2017.0064
- Karel Kellens, Martin Baumers, Timothy G. Gutowski, William Flanagan, Reid Lifset, Joost R. Duflou. Environmental Dimensions of Additive Manufacturing: Mapping Application Domains and Their Environmental Implications. Journal of Industrial Ecology 2017, 21 (S1) , S49-S68. https://doi.org/10.1111/jiec.12629
- Daniel Whitley, R. Scott Eidson, Ivan Rudek, Sompop Bencharit. In-office fabrication of dental implant surgical guides using desktop stereolithographic printing and implant treatment planning software: A clinical report. The Journal of Prosthetic Dentistry 2017, 118 (3) , 256-263. https://doi.org/10.1016/j.prosdent.2016.10.017
- Andrea L. Kadilak, Jessica C. Rehaag, Cameron A. Harrington, Leslie M. Shor. A 3D-printed microbial cell culture platform with in situ PEGDA hydrogel barriers for differential substrate delivery. Biomicrofluidics 2017, 11 (5) , 054109. https://doi.org/10.1063/1.5003477
- Chee Kai Chua, Chee How Wong, Wai Yee Yeong. Benchmarking for Additive Manufacturing. 2017,,, 181-212. https://doi.org/10.1016/B978-0-12-813489-4.00008-8
- . Standards, Quality Control, and Measurement Sciences in 3D Printing and Additive Manufacturing. 2017,,https://doi.org/
- Rowan P. Rimington, Andrew J. Capel, Steven D. R. Christie, Mark P. Lewis. Biocompatible 3D printed polymers via fused deposition modelling direct C 2 C 12 cellular phenotype in vitro. Lab on a Chip 2017, 17 (17) , 2982-2993. https://doi.org/10.1039/C7LC00577F
- Javeed Shaikh Mohammed. Applications of 3D printing technologies in oceanography. Methods in Oceanography 2016, 17 , 97-117. https://doi.org/10.1016/j.mio.2016.08.001
- Giovanni Anthony, Ju-Ahng Lee, . An Optimized Small Tissue Handling System for Immunohistochemistry and In Situ Hybridization. PLOS ONE 2016, 11 (8) , e0159991. https://doi.org/10.1371/journal.pone.0159991
- David Rejeski. 3-D printing: green or not?. XRDS: Crossroads, The ACM Magazine for Students 2016, 22 (3) , 68-69. https://doi.org/10.1145/2893507
- N. P. Macdonald, F. Zhu, C. J. Hall, J. Reboud, P. S. Crosier, E. E. Patton, D. Wlodkowic, J. M. Cooper. Assessment of biocompatibility of 3D printed photopolymers using zebrafish embryo toxicity assays. Lab on a Chip 2016, 16 (2) , 291-297. https://doi.org/10.1039/C5LC01374G
- Sidra Waheed, Joan M. Cabot, Niall P. Macdonald, Trevor Lewis, Rosanne M. Guijt, Brett Paull, Michael C. Breadmore. 3D printed microfluidic devices: enablers and barriers. Lab on a Chip 2016, 16 (11) , 1993-2013. https://doi.org/10.1039/C6LC00284F



