Using Fluorescence Spectroscopy To Identify Milk from Grass-Fed Dairy Cows and To Monitor Its PhotodegradationClick to copy article linkArticle link copied!
- Ujjal BhattacharjeeUjjal BhattacharjeeDepartment of Chemistry, Iowa State University (ISU), Ames, Iowa 50011, United StatesAmes Laboratory, United States Department of Energy, Ames, Iowa 50011, United StatesMore by Ujjal Bhattacharjee
- Danielle JarashowDanielle JarashowDepartment of Chemistry, Iowa State University (ISU), Ames, Iowa 50011, United StatesMore by Danielle Jarashow
- Thomas A. CaseyThomas A. CaseyDepartment of Chemistry, Iowa State University (ISU), Ames, Iowa 50011, United StatesMore by Thomas A. Casey
- Jacob W. Petrich*Jacob W. Petrich*E-mail: [email protected]Department of Chemistry, Iowa State University (ISU), Ames, Iowa 50011, United StatesAmes Laboratory, United States Department of Energy, Ames, Iowa 50011, United StatesMore by Jacob W. Petrich
- Mark A. Rasmussen*Mark A. Rasmussen*E-mail: [email protected]Leopold Center for Sustainable Agriculture, Iowa State University (ISU), Ames, Iowa 50011, United StatesMore by Mark A. Rasmussen
Abstract

Owing to its high ω-3 fatty acid content, milk from grass-fed dairy cows is becoming increasingly more attractive to consumers. Consequently, it is important to identify the origins of such products and to measure their content, at least relative to some standard. To date, chromatography has been the most extensively used technique. Sample preparation and cost, however, often reduce its widespread applicability. Here, we report the effectiveness of fluorescence spectroscopy for such quantification by measuring the amount of chlorophyll metabolites in the sample. Their content is significantly higher for milk from grass-fed cows compared to milk from grain/silage-fed cows. It is 0.11–0.13 μM in milk samples from grass-fed cows, whereas in milk from cows fed grain/silage rations, the concentration was 0.01–0.04 μM. In various organic milk samples, the chlorophyll metabolite concentration was in the range of 0.07–0.09 μM. In addition, we explored the mechanisms of photodegradation of milk. Riboflavin and chlorophyll metabolites act as photosensitizers in milk for type-I and type-II reactions, respectively. It was also observed that the presence of high levels of chlorophyll metabolites can synergistically degrade riboflavin, contributing to the degradation of milk quality.
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This article is cited by 7 publications.
- Áine M. Ní Fhuaráin, Colm P. O’Donnell, Jiani Luo, Aoife A. Gowen. A Review on MIR, NIR, Fluorescence and Raman Spectroscopy Combined with Chemometric Modeling to Predict the Functional Properties of Raw Bovine Milk. ACS Food Science & Technology 2024, 4
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- Amy Birkinshaw, Michael Sutter, Beat Reidy, Laurence Jungo, Stefanie Mueller, Michael Kreuzer, Melissa Terranova, . Evaluation and quantification of associations between commonly suggested milk biomarkers and the proportion of grassland-based feeds in the diets of dairy cows. PLOS ONE 2023, 18
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- Elena Hayes, Derek Greene, Colm O’Donnell, Norah O’Shea, Mark A. Fenelon. Spectroscopic technologies and data fusion: Applications for the dairy industry. Frontiers in Nutrition 2023, 9 https://doi.org/10.3389/fnut.2022.1074688
- Kalyan Santra, Anthony Song, Jacob W Petrich, Mark A Rasmussen. The degradation of chlorophyll pigments in dairy silage: the timeline of anaerobic fermentation. Journal of the Science of Food and Agriculture 2021, 101
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, 2863-2868. https://doi.org/10.1002/jsfa.10917
- A. Logan, L. Walter, P. Watkins. Analytical Methods. 2020, 443-476. https://doi.org/10.1007/978-3-030-48686-0_13
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