Using Neutron Reflectometry to Discern the Structure of Fibrinogen Adsorption at the Stainless Steel/Aqueous InterfaceClick to copy article linkArticle link copied!
Abstract
Neutron reflectometry has been successfully used to study adsorption on a stainless steel surface by means of depositing a thin steel film on silicon. The film was characterized using XPS (X-ray photoelectron spectroscopy), TOF-SIMS (time-of-flight secondary ion mass spectrometry), and GIXRD (grazing incidence X-ray diffraction), demonstrating the retention both of the austenitic phase and of the required composition for 316L stainless steel. The adsorption of fibrinogen from a physiologically-relevant solution onto the steel surface was studied using neutron reflectometry and QCM (quartz crystal microbalance) and compared to that on a deposited chromium oxide surface. It was found that the protein forms an irreversibly bound layer at low concentrations, with maximum protein concentration a distance of around 20 Å from the surface. Evidence for a further diffuse reversibly-bound layer forming at higher concentrations was also observed. Both the structure of the layer revealed by the neutron reflectometry data and the high water retention predicted by the QCM data suggest that there is a significant extent of protein unfolding upon adsorption. A lower extent of adsorption was seen on the chromium surfaces, although the adsorbed layer structures were similar, suggesting comparable adsorption mechanisms.
Cited By
This article is cited by 6 publications.
- Maths Karlsson, Lars-Gunnar Johansson, Laura Mazzei, Jan Froitzheim, Max Wolff. Neutron Reflectivity in Corrosion Research on Metals. ACS Materials Au 2024, 4
(4)
, 346-353. https://doi.org/10.1021/acsmaterialsau.4c00011
- Richard
M. Alloway, Jennings Mong, Izaak W. Jephson, Mary H. Wood, Michael T. L. Casford, Peter Grice, Sorin V. Filip, Ibrahim E. Salama, Colm Durkan, Stuart M. Clarke. Adsorption of 4-n-Nonylphenol, Carvacrol, and Ethanol onto Iron Oxide from Nonaqueous Hydrocarbon Solvents. Langmuir 2019, 35
(36)
, 11662-11669. https://doi.org/10.1021/acs.langmuir.9b01863
- Mary H. Wood, Elizabeth K. Humphreys, Rebecca J. L. Welbourn. Structural Changes in Adsorbed Cytochrome c upon Applied Potential Characterized by Neutron Reflectometry. Langmuir 2019, 35
(18)
, 6055-6063. https://doi.org/10.1021/acs.langmuir.9b00691
- Mary H. Wood, Charanee Galabada Payagalage, Thomas Geue. Bovine Serum Albumin and Fibrinogen Adsorption at the 316L Stainless Steel/Aqueous Interface. The Journal of Physical Chemistry B 2018, 122
(19)
, 5057-5065. https://doi.org/10.1021/acs.jpcb.8b01347
- R.J.L. Welbourn, S.M. Clarke. New insights into the solid–liquid interface exploiting neutron reflectivity. Current Opinion in Colloid & Interface Science 2019, 42 , 87-98. https://doi.org/10.1016/j.cocis.2019.03.007
- Mary Wood, Stuart Clarke. Neutron Reflectometry for Studying Corrosion and Corrosion Inhibition. Metals 2017, 7
(8)
, 304. https://doi.org/10.3390/met7080304
Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.
Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.
The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated.