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Upregulation of Glycans Containing 3′ Fucose in a Subset of Pancreatic Cancers Uncovered Using Fusion-Tagged Lectins
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    Upregulation of Glycans Containing 3′ Fucose in a Subset of Pancreatic Cancers Uncovered Using Fusion-Tagged Lectins
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    Van Andel Research Institute, Grand Rapids, Michigan 49503, United States
    Palo Alto Research Center, Palo Alto, California 94304, United States
    § Spectrum Health, Grand Rapids, Michigan 49503, United States
    Emory University, Atlanta, Georgia 30322, United States
    Drexel University, Philadelphia, Pennsylvania 19104, United States
    # University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213, United States
    *Mailing address: Van Andel Research Institute, 333 Bostwick NE, Grand Rapids, MI 49503. Telephone: 616-234-5268. E-mail: [email protected]
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    Journal of Proteome Research

    Cite this: J. Proteome Res. 2015, 14, 6, 2594–2605
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    https://doi.org/10.1021/acs.jproteome.5b00142
    Published May 4, 2015
    Copyright © 2015 American Chemical Society

    Abstract

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    The fucose post-translational modification is frequently increased in pancreatic cancer, thus forming the basis for promising biomarkers, but a subset of pancreatic cancer patients does not elevate the known fucose-containing biomarkers. We hypothesized that such patients elevate glycan motifs with fucose in linkages and contexts different from the known fucose-containing biomarkers. We used a database of glycan array data to identify the lectins CCL2 to detect glycan motifs with fucose in a 3′ linkage; CGL2 for motifs with fucose in a 2′ linkage; and RSL for fucose in all linkages. We used several practical methods to test the lectins and determine the optimal mode of detection, and we then tested whether the lectins detected glycans in pancreatic cancer patients who did not elevate the sialyl-Lewis A glycan, which is upregulated in ∼75% of pancreatic adenocarcinomas. Patients who did not upregulate sialyl-Lewis A, which contains fucose in a 4′ linkage, tended to upregulate fucose in a 3′ linkage, as detected by CCL2, but they did not upregulate total fucose or fucose in a 2′ linkage. CCL2 binding was high in cancerous epithelia from pancreatic tumors, including areas negative for sialyl-Lewis A and a related motif containing 3′ fucose, sialyl-Lewis X. Thus, glycans containing 3′ fucose may complement sialyl-Lewis A to contribute to improved detection of pancreatic cancer. Furthermore, the use of panels of recombinant lectins may uncover details about glycosylation that could be important for characterizing and detecting cancer.

    Copyright © 2015 American Chemical Society

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    Supporting Information

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    Table S1: Antibodies and proteins used. Table S2: Primer information. Table S3: Binders to alpha 1,2 fucose. Table S4: Binders to alpha 1,3 fucose. Table S5: Binders to alpha 1,4 fucose. Table S6: Binders to alpha 1,6 fucose. Figure S1: Purification and evaluation of CGL2 and CCL2. Figure S2: Confirmation of glycan binding. Figure S3: Immunohistochemistry analysis of sLeA, sLeX, and CCL2. The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.jproteome.5b00142.

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    Journal of Proteome Research

    Cite this: J. Proteome Res. 2015, 14, 6, 2594–2605
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acs.jproteome.5b00142
    Published May 4, 2015
    Copyright © 2015 American Chemical Society

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