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Quantitative Proteomics of Zea mays Hybrids Exhibiting Different Levels of Heterosis

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‡ § Division of Biological Sciences, Department of Biochemistry, and §The Charles W Gehrke Proteomics Center, University of Missouri, Columbia, Missouri 65211, United States
*214 Bond Life Sciences Center, 1201 Rollins Street, Columbia, MO 65211. Tel: 573-884-7374. Fax: 573-884-9676. E-mail: [email protected]
Cite this: J. Proteome Res. 2016, 15, 8, 2445–2454
Publication Date (Web):June 14, 2016
https://doi.org/10.1021/acs.jproteome.5b01120
Copyright © 2016 American Chemical Society

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    Abstract

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    Maize hybrids exhibiting heterosis (hybrid vigor) were generated from inbred parents with increasing genetic distance. B73 was used as the common female parent in crosses with N192 (low heterosis), MO17 (high-heterosis 1), and NC350 (high-heterosis 2). Total and mitochondria-enriched proteomes were analyzed from ear shoots of field-grown hybrids and their inbred parents. GeLCMS (1D SDS-PAGE fractionation, trypsin digestion, LTQ Orbitrap nano-RP-LC MS/MS) was used to analyze proteins, and spectral counting was used for quantitation. In total, 3,568 proteins were identified and quantified in hybrids including 2,489 in the mitochondria-enriched fraction and 2,162 in the total protein fraction. Sixty-one proteins were differentially abundant (p < 0.05) in one or both of the high-heterosis hybrids compared with the low-heterosis hybrid. For the total proteome, eight of these showed similar trends in abundance in both of the higher-heterosis hybrids. Nine proteins showed this heterosis-correlated pattern in the mitochondrial proteome, including a mitochondria-associated target of rapamycin (TOR) protein. Although differentially abundant proteins belong to various pathways, protein, and RNA metabolism, and stress responsive proteins were the major classes changed in response to increasing heterosis.

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    Supporting Information

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.jproteome.5b01120.

    • Functional classification of all proteins identified in the study (PDF)

    • Detailed protein and peptide data for total protein proteome (ZIP)

    • Detailed protein and peptide data for hybrid mitochondrial proteomes (ZIP)

    • Detailed protein and peptide data for inbred parent mitochondrial proteomes (ZIP)

    • MaizeDB-GRM accession numbers annotated using BLASTP comparison to plant orthologues (ZIP)

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    This article is cited by 8 publications.

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    2. Zejun Mo, Yuanyuan Pu, Junhao Zhou, Zonglin Tian, Jianhui Teng, Qian Chen, Lili Duan, Renxiang Liu. Effect of the over-dominant expression of proteins on nicotine heterosis via proteomic analysis. Scientific Reports 2021, 11 (1) https://doi.org/10.1038/s41598-021-00614-x
    3. Bikram Datt Pant, Sunhee Oh, Hee-Kyung Lee, Raja Sekhar Nandety, Kirankumar S. Mysore. Antagonistic Regulation by CPN60A and CLPC1 of TRXL1 that Regulates MDH Activity Leading to Plant Disease Resistance and Thermotolerance. Cell Reports 2020, 33 (11) , 108512. https://doi.org/10.1016/j.celrep.2020.108512
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    6. Bo Yang, Andrew Thomas, C. Greenlief. Comparative Proteomic Analysis Unveils Critical Pathways Underlying the Role of Nitrogen Fertilizer Treatment in American Elderberry. Proteomes 2019, 7 (1) , 10. https://doi.org/10.3390/proteomes7010010
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    8. Dana Holá, Monika Benešová, Lukáš Fischer, Daniel Haisel, František Hnilička, Helena Hniličková, Petr L. Jedelský, Marie Kočová, Dagmar Procházková, Olga Rothová, Lenka Tůmová, Naďa Wilhelmová, . The disadvantages of being a hybrid during drought: A combined analysis of plant morphology, physiology and leaf proteome in maize. PLOS ONE 2017, 12 (4) , e0176121. https://doi.org/10.1371/journal.pone.0176121

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