Direct Assembly and Metal-Ion Binding Properties of Oxytocin Monolayer on Gold Surfaces
- Evgeniy MervinetskyEvgeniy MervinetskyInstitute of Chemistry and Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, E. Safra Campus, 91904 Jerusalem, IsraelMore by Evgeniy Mervinetsky
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- Israel AlshanskiIsrael AlshanskiInstitute of Chemistry and Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, E. Safra Campus, 91904 Jerusalem, IsraelMore by Israel Alshanski
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- Jörg BuchwaldJörg BuchwaldInstitute for Materials Science and Max Bergmann Center of Biomaterials, Hallwachsstraße 3, 01062 Dresden, GermanyMore by Jörg Buchwald
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- Arezoo DianatArezoo DianatInstitute for Materials Science and Max Bergmann Center of Biomaterials, Hallwachsstraße 3, 01062 Dresden, GermanyMore by Arezoo Dianat
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- Ivor LončarićIvor LončarićRuđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, CroatiaMore by Ivor Lončarić
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- Predrag LazićPredrag LazićRuđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, CroatiaMore by Predrag Lazić
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- Željko Crljen*Željko Crljen*E-mail: [email protected] (Z.C.).Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, CroatiaMore by Željko Crljen
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- Rafael Gutierrez*Rafael Gutierrez*E-mail: [email protected] (R.G.).Institute for Materials Science and Max Bergmann Center of Biomaterials, Hallwachsstraße 3, 01062 Dresden, GermanyMore by Rafael Gutierrez
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- Gianaurelio CunibertiGianaurelio CunibertiInstitute for Materials Science and Max Bergmann Center of Biomaterials, Hallwachsstraße 3, 01062 Dresden, GermanyDresden Center for Computational Materials Science, TU Dresden, 01062 Dresden, GermanyCenter for Advancing Electronics Dresden, TU Dresden, 01062 Dresden, GermanyMore by Gianaurelio Cuniberti
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- Mattan HurevichMattan HurevichInstitute of Chemistry and Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, E. Safra Campus, 91904 Jerusalem, IsraelMore by Mattan Hurevich
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- Shlomo Yitzchaik*Shlomo Yitzchaik*E-mail: [email protected] (S.Y.).Institute of Chemistry and Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, E. Safra Campus, 91904 Jerusalem, IsraelMore by Shlomo Yitzchaik
Abstract
Peptides are very common recognition entities that are usually attached to surfaces using multistep processes. These processes require modification of the native peptides and of the substrates. Using functional groups in native peptides for their assembly on surfaces without affecting their biological activity can facilitate the preparation of biosensors. Herein, we present a simple single-step formation of native oxytocin monolayer on gold surface. These surfaces were characterized by atomic force spectroscopy, spectroscopic ellipsometry, and X-ray photoelectron spectroscopy. We took advantage of the native disulfide bridge of the oxytocin for anchoring the peptide to the Au surface, while preserving the metal-ion binding properties. Self-assembled oxytocin monolayer was used by electrochemical impedance spectroscopy for metal-ion sensing leading to subnanomolar sensitivities for zinc or copper ions.
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