Modulating Mechanical Properties of Collagen–Lignin Composites
- Jorge A. BelgodereJorge A. BelgodereBiological and Agricultural Engineering, Louisiana State University, 149 E.B. Doran Hall, Baton Rouge, Louisiana 70803, United StatesMore by Jorge A. Belgodere,
- Syed A. ZaminSyed A. ZaminBiological and Agricultural Engineering, Louisiana State University, 149 E.B. Doran Hall, Baton Rouge, Louisiana 70803, United StatesMore by Syed A. Zamin,
- Ryan M. KalinoskiRyan M. KalinoskiBiosystems and Agricultural Engineering, University of Kentucky, 128 C.E. Barnhart Building, Lexington, Kentucky 40546, United StatesMore by Ryan M. Kalinoski,
- Carlos E. AsteteCarlos E. AsteteBiological and Agricultural Engineering, Louisiana State University, 149 E.B. Doran Hall, Baton Rouge, Louisiana 70803, United StatesMore by Carlos E. Astete,
- Joseph C. PenrodJoseph C. PenrodBiological and Agricultural Engineering, Louisiana State University, 149 E.B. Doran Hall, Baton Rouge, Louisiana 70803, United StatesMore by Joseph C. Penrod,
- Katie M. HamelKatie M. HamelBiological and Agricultural Engineering, Louisiana State University, 149 E.B. Doran Hall, Baton Rouge, Louisiana 70803, United StatesMore by Katie M. Hamel,
- Bert C. LynnBert C. LynnChemistry, University of Kentucky, 125 Chemistry/Physics Building, Lexington, Kentucky 40506, United StatesMore by Bert C. Lynn,
- Jai S. RudraJai S. RudraDepartment of Pharmacology and Toxicology, University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555, United StatesMore by Jai S. Rudra,
- Jian Shi*Jian Shi*E-mail: [email protected]Chemistry, University of Kentucky, 125 Chemistry/Physics Building, Lexington, Kentucky 40506, United StatesMore by Jian Shi, and
- Jangwook P. Jung*Jangwook P. Jung*E-mail: [email protected]Biosystems and Agricultural Engineering, University of Kentucky, 128 C.E. Barnhart Building, Lexington, Kentucky 40546, United StatesMore by Jangwook P. Jung
Abstract
Three-dimensional matrices of collagen type I (Col I) are widely used in tissue engineering applications for its abundance in many tissues, bioactivity with many cell types, and excellent biocompatibility. Inspired by the structural role of lignin in a plant tissue, we found that sodium lignosulfonate (SLS) and an alkali-extracted lignin from switchgrass (SG) increased the stiffness of Col I gels. SLS and SG enhanced the stiffness of Col I gels from 52 to 670 Pa and 52 to 320 Pa, respectively, and attenuated shear-thinning properties, with the formulation of 1.8 mg/mL Col I and 5.0 mg/mL SLS or SG. In 2D cultures, the cytotoxicity of collagen–SLS to adipose-derived stromal cells was not observed and the cell viability was maintained over 7 days in 3D cultures. Collagen–SLS composites did not elicit immunogenicity when compared to SLS-only groups. Our collagen–SLS composites present a case that exploits lignins as an enhancer of mechanical properties of Col I without adverse cytotoxicity and immunogenicity for in vitro scaffolds or in vivo tissue repairs.