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In Vitro Culture and Directed Osteogenic Differentiation of Human Pluripotent Stem Cells on Peptides-Decorated Two-Dimensional Microenvironment

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Department of Oral and Maxillofacial Surgery, Laboratory of Interdisciplinary Studies, School and Hospital of Stomatology, Peking University, Beijing 100081, People’s Republic of China
Second Dental Center, School and Hospital of Stomatology, Peking University, No. 66 An-Li Road, Chao-Yang District, Beijing 100081, People’s Republic of China
§ Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, People’s Republic of China
Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, People’s Republic of China
Chongqing Key laboratory for Oral Diseases and Biomedical Sciences, Chongqing Medical University, Chongqing 400015, People’s Republic of China
*Phone/Fax: +86 10 82195780. E-mail: [email protected]
*Phone/Fax: +86 10 82196271. E-mail: [email protected]
Cite this: ACS Appl. Mater. Interfaces 2015, 7, 8, 4560–4572
Publication Date (Web):February 11, 2015
https://doi.org/10.1021/acsami.5b00188
Copyright © 2015 American Chemical Society
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Abstract

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Human pluripotent stem cells (hPSCs) are a promising cell source with pluripotency and capacity to differentiate into all human somatic cell types. Designing simple and safe biomaterials with an innate ability to induce osteoblastic lineage from hPSCs is desirable to realize their clinical adoption in bone regenerative medicine. To address the issue, here we developed a fully defined synthetic peptides-decorated two-dimensional (2D) microenvironment via polydopamine (pDA) chemistry and subsequent carboxymethyl chitosan (CMC) grafting to enhance the culture and osteogenic potential of hPSCs in vitro. The hPSCs including human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were successfully cultured on the peptides-decorated surface without Matrigel and ECM protein coating and underwent promoted osteogenic differentiation in vitro, determined from the alkaline phosphate (ALP) activity, gene expression, and protein production as well as calcium deposit amount. It was found that directed osteogenic differentiation of hPSCs was achieved through a peptides-decorated niche. This chemically defined and safe 2D microenvironment, which facilitates proliferation and osteo-differentiation of hPSCs, not only helps to accelerate the translational perspectives of hPSCs but also provides tissue-specific functions such as directing stem cell differentiation commitment, having great potential in bone tissue engineering and opening new avenues for bone regenerative medicine.

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High-resolution spectra of carbon peaks for the pristine and decorated PS substrates; hiPSCs and hESCs morphology on the pure CMC substrate at day 0; hiPSCs and hESCs morphology of different peptides-decorated surfaces from day 1 to day 7; bone matrix mineralization of hMSCs treated with BMP-7 after inducing for 28 days in vitro; primer sequence (5′-3′) for the quantitative real-time PCR; elemental composition of the pristine and decorated PS substrates determined by XPS analysis; summary of surface properties of the decorated PS plates determined by means of AFM peak force QNM tapping in air. This material is available free of charge via the Internet at http://pubs.acs.org.

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  5. Chenshuang Li, Zane Mills, Zhong Zheng. Novel cell sources for bone regeneration. MedComm 2021, 2 (2) , 145-174. https://doi.org/10.1002/mco2.51
  6. Lluís Oliver‐Cervelló, Helena Martin‐Gómez, Carlos Mas‐Moruno. New trends in the development of multifunctional peptides to functionalize biomaterials. Journal of Peptide Science 2021, 9 https://doi.org/10.1002/psc.3335
  7. Zhangfan Ding, Xun Pan, Xiaoyi Wang, Huixu Xie, Qingsong Ye. Interactions between induced pluripotent stem cells and stem cell niche augment osteogenesis and bone regeneration. Smart Materials in Medicine 2021, 2 , 196-208. https://doi.org/10.1016/j.smaim.2021.07.002
  8. Israa Ahmed Radwan, Dina Rady, Marwa M. S. Abbass, Sara El Moshy, Nermeen AbuBakr, Christof E. Dörfer, Karim M. Fawzy El-Sayed. Induced Pluripotent Stem Cells in Dental and Nondental Tissue Regeneration: A Review of an Unexploited Potential. Stem Cells International 2020, 2020 , 1-24. https://doi.org/10.1155/2020/1941629
  9. Jennifer Patterson. Peptide-functionalized Biomaterials with Osteoinductive or Anti-biofilm Activity. 2020,,, 129-168. https://doi.org/10.1007/978-3-030-34471-9_6
  10. Sajeesh Kumar Madhurakkat Perikamana, Taufiq Ahmad, Sangmin Lee, Heungsoo Shin. Frontiers in research for bone biomaterials. 2020,,, 307-332. https://doi.org/10.1016/B978-0-08-102478-2.00013-1
  11. Marco d’Ischia, Alessandra Napolitano, Alessandro Pezzella. Pyrroles and Their Benzo Derivatives: Applications. 2020,,https://doi.org/10.1016/B978-0-12-818655-8.00022-6
  12. Jun Chen, Xuenong Zou. Self-assemble peptide biomaterials and their biomedical applications. Bioactive Materials 2019, 4 , 120-131. https://doi.org/10.1016/j.bioactmat.2019.01.002
  13. Taylor B. Bertucci, Guohao Dai. Biomaterial Engineering for Controlling Pluripotent Stem Cell Fate. Stem Cells International 2018, 2018 , 1-12. https://doi.org/10.1155/2018/9068203
  14. Siqi Zhang, Yuhua Sun, Yi Sui, Yan Li, Zuyuan Luo, Xiao Xu, Ping Zhou, Shicheng Wei. Determining Osteogenic Differentiation Efficacy of Pluripotent Stem Cells by Telomerase Activity. Tissue Engineering and Regenerative Medicine 2018, 15 (6) , 751-760. https://doi.org/10.1007/s13770-018-0138-6
  15. Yue Yang, Zuyuan Luo, Ying Zhao. Osteostimulation scaffolds of stem cells: BMP-7-derived peptide-decorated alginate porous scaffolds promote the aggregation and osteo-differentiation of human mesenchymal stem cells. Biopolymers 2018, 109 (7) , e23223. https://doi.org/10.1002/bip.23223
  16. Riham Fliefel, Michael Ehrenfeld, Sven Otto. Induced pluripotent stem cells (iPSCs) as a new source of bone in reconstructive surgery: A systematic review and meta-analysis of preclinical studies. Journal of Tissue Engineering and Regenerative Medicine 2018, 12 (7) , 1780-1797. https://doi.org/10.1002/term.2697
  17. Zuyuan Luo, Siqi Zhang, Jijia Pan, Rui Shi, Hao Liu, Yalin Lyu, Xiao Han, Yan Li, Yue Yang, Zhixiu Xu, Yi Sui, En Luo, Yuanyuan Zhang, Shicheng Wei. Time-responsive osteogenic niche of stem cells: A sequentially triggered, dual-peptide loaded, alginate hybrid system for promoting cell activity and osteo-differentiation. Biomaterials 2018, 163 , 25-42. https://doi.org/10.1016/j.biomaterials.2018.02.025
  18. Yi Deng, Shicheng Wei, Lei Yang, Weizhong Yang, Matthew S. Dargusch, Zhi‐Gang Chen. A Novel Hydrogel Surface Grafted With Dual Functional Peptides for Sustaining Long‐Term Self‐Renewal of Human Induced Pluripotent Stem Cells and Manipulating Their Osteoblastic Maturation. Advanced Functional Materials 2018, 28 (11) , 1705546. https://doi.org/10.1002/adfm.201705546
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  21. Maria Csobonyeiova, Stefan Polak, Radoslav Zamborsky, Lubos Danisovic. iPS cell technologies and their prospect for bone regeneration and disease modeling: A mini review. Journal of Advanced Research 2017, 8 (4) , 321-327. https://doi.org/10.1016/j.jare.2017.02.004
  22. Qingqing Wu, Bo Yang, Kevin Hu, Cong Cao, Yi Man, Ping Wang. Deriving Osteogenic Cells from Induced Pluripotent Stem Cells for Bone Tissue Engineering. Tissue Engineering Part B: Reviews 2017, 23 (1) , 1-8. https://doi.org/10.1089/ten.teb.2015.0559
  23. Eun Young Heo, Na Re Ko, Min Soo Bae, Sang Jin Lee, Byung-Joon Choi, Jung Ho Kim, Hyung Keun Kim, Su A Park, Il Keun Kwon. Novel 3D printed alginate–BFP1 hybrid scaffolds for enhanced bone regeneration. Journal of Industrial and Engineering Chemistry 2017, 45 , 61-67. https://doi.org/10.1016/j.jiec.2016.09.003
  24. Yan Li, Zuyuan Luo, Xiao Xu, Yongliang Li, Siqi Zhang, Ping Zhou, Yi Sui, Minjie Wu, En Luo, Shicheng Wei. Aspirin enhances the osteogenic and anti-inflammatory effects of human mesenchymal stem cells on osteogenic BFP-1 peptide-decorated substrates. Journal of Materials Chemistry B 2017, 5 (34) , 7153-7163. https://doi.org/10.1039/C7TB01732D
  25. Nikolaus Knoepffler, Tade Matthias Spranger, Nikolai Münch, Martin O’Malley. Ethics and Law in Regenerative Medicine. 2016,,, 35-49. https://doi.org/10.1007/978-3-319-28293-0_3
  26. Anxiu Xu, Liwei Zhou, Yi Deng, Xianshen Chen, Xiaoling Xiong, Feng Deng, Shicheng Wei. A carboxymethyl chitosan and peptide-decorated polyetheretherketone ternary biocomposite with enhanced antibacterial activity and osseointegration as orthopedic/dental implants. Journal of Materials Chemistry B 2016, 4 (10) , 1878-1890. https://doi.org/10.1039/C5TB02782A
  27. K. Hynes, D. Menichanin, R. Bright, S. Ivanovski, D.W. Hutmacher, S. Gronthos, P.M. Bartold. Induced Pluripotent Stem Cells. Journal of Dental Research 2015, 94 (11) , 1508-1515. https://doi.org/10.1177/0022034515599769
  28. Xiangxin Lou. Induced Pluripotent Stem Cells as a new Strategy for Osteogenesis and Bone Regeneration. Stem Cell Reviews and Reports 2015, 11 (4) , 645-651. https://doi.org/10.1007/s12015-015-9594-8

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