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Real-Time Evaluation of Live Cancer Cells by an in Situ Surface Plasmon Resonance and Electrochemical Study
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    Real-Time Evaluation of Live Cancer Cells by an in Situ Surface Plasmon Resonance and Electrochemical Study
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    State Key Laboratory of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University, Nanjing 210096, China
    Laboratory Animal Center, Nantong University, Nantong 226001, China
    *Phone/Fax: +86-25-83792177. E-mail: [email protected]
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    ACS Applied Materials & Interfaces

    Cite this: ACS Appl. Mater. Interfaces 2015, 7, 44, 24848–24854
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    https://doi.org/10.1021/acsami.5b08066
    Published October 22, 2015
    Copyright © 2015 American Chemical Society

    Abstract

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    This work presents a new strategy of the combination of surface plasmon resonance (SPR) and electrochemical study for real-time evaluation of live cancer cells treated with daunorubicin (DNR) at the interface of the SPR chip and living cancer cells. The observations demonstrate that the SPR signal changes could be closely related to the morphology and mass changes of adsorbed cancer cells and the variation of the refractive index of the medium solution. The results of light microscopy images and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide studies also illustrate the release or desorption of HepG2 cancer cells, which were due to their apoptosis after treatment with DNR. It is evident that the extracellular concentration of DNR residue can be readily determined through electrochemical measurements. The decreases in the magnitudes of SPR signals were linearly related to cell survival rates, and the combination of SPR with electrochemical study could be utilized to evaluate the potential therapeutic efficiency of bioactive agents to cells. Thus, this label-free, real-time SPR–electrochemical detection technique has great promise in bioanalysis or monitoring of relevant treatment processes in clinical applications.

    Copyright © 2015 American Chemical Society

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    Supporting Information

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    The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acsami.5b08066.

    • Calculated values for the refractive index of ethanol solutions, SPR signals of DNR-treated HepG2 cells with and without electrochemical samplings, scan voltage-based SPR signals, and an in vitro cytotoxicity study using the MTT assay (PDF)

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    ACS Applied Materials & Interfaces

    Cite this: ACS Appl. Mater. Interfaces 2015, 7, 44, 24848–24854
    Click to copy citationCitation copied!
    https://doi.org/10.1021/acsami.5b08066
    Published October 22, 2015
    Copyright © 2015 American Chemical Society

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