Simultaneous Detection of Antibiotic Resistance Genes on Paper-Based Chip Using [Ru(phen)2dppz]2+ Turn-on Fluorescence ProbeClick to copy article linkArticle link copied!
Abstract
Antibiotic resistance, the ability of some bacteria to resist antibiotic drugs, has been a major global health burden due to the extensive use of antibiotic agents. Antibiotic resistance is encoded via particular genes; hence the specific detection of these genes is necessary for diagnosis and treatment of antibiotic resistant cases. Conventional methods for monitoring antibiotic resistance genes require the sample to be transported to a central laboratory for tedious and sophisticated tests, which is grueling and time-consuming. We developed a paper-based chip, integrated with loop-mediated isothermal amplification (LAMP) and the “light switch” molecule [Ru(phen)2dppz]2+, to conduct turn-on fluorescent detection of antibiotic resistance genes. In this assay, the amplification reagents can be embedded into test spots of the chip in advance, thus simplifying the detection procedure. [Ru(phen)2dppz]2+ was applied to intercalate into amplicons for product analysis, enabling this assay to be operated in a wash-free format. The paper-based detection device exhibited a limit of detection (LOD) as few as 100 copies for antibiotic resistance genes. Meanwhile, it could detect antibiotic resistance genes from various bacteria. Noticeably, the approach can be applied to other genes besides antibiotic resistance genes by simply changing the LAMP primers. Therefore, this paper-based chip has the potential for point-of-care (POC) applications to detect various gene samples, especially in resource-limited conditions.
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(1)
, 292-300. https://doi.org/10.1021/acs.analchem.3c04060
- Shun Wang, Mingyuan Sun, Yunhong Zhang, Hao Ji, Jianwei Gao, Shuai Song, Jun Sun, Hong Liu, Yu Zhang, Lin Han. Ultrasensitive Antibiotic Perceiving Based on Aptamer-Functionalized Ultraclean Graphene Field-Effect Transistor Biosensor. Analytical Chemistry 2022, 94
(42)
, 14785-14793. https://doi.org/10.1021/acs.analchem.2c03732
- Lijuan Liu, Xin Li, Qingfeng Yao, Yachen Hu, Hongwei Sun, Lizhi Zhang, Jingming Gong. Temperature-Responsive Nanocarrier-Regulated Alternative Release of “Cargos” for a Multiplex Photoelectrochemical Bioassay of Antibiotic-Resistant Genes. Analytical Chemistry 2022, 94
(40)
, 14061-14070. https://doi.org/10.1021/acs.analchem.2c03698
- Ke-Jia Wu, Chun Wu, Feng Chen, Sha-Sha Cheng, Dik-Lung Ma, Chung-Hang Leung. Time-Resolved Luminescent High-Throughput Screening Platform for Lysosomotropic Compounds in Living Cells. ACS Sensors 2021, 6
(1)
, 166-174. https://doi.org/10.1021/acssensors.0c02046
- Xin Li, Junmiao Lu, Lizhen Feng, Lizhi Zhang, Jingming Gong. Smart pH-Regulated Switchable Nanoprobes for Photoelectrochemical Multiplex Detection of Antibiotic Resistance Genes. Analytical Chemistry 2020, 92
(16)
, 11476-11483. https://doi.org/10.1021/acs.analchem.0c02839
- Ruijin Zeng, Zhongbin Luo, Lijia Zhang, Dianping Tang. Platinum Nanozyme-Catalyzed Gas Generation for Pressure-Based Bioassay Using Polyaniline Nanowires-Functionalized Graphene Oxide Framework. Analytical Chemistry 2018, 90
(20)
, 12299-12306. https://doi.org/10.1021/acs.analchem.8b03889
- Rujing Kang, Ren Li, Juma Mjengi, Zohair Abbas, Yihong Song, Lei Zhang. A tiny sample rapid visual detection technology for imidacloprid resistance in Aphis gossypii by CRISPR/Cas12a. Science of The Total Environment 2024, 951 , 175712. https://doi.org/10.1016/j.scitotenv.2024.175712
- Qing Tang, Hanren Chen, Bihong Diao, Zuoyan Qin, Huaze Shao, Runhui Zhang, Lihong Liu. PCR-free and visual detection of antibiotic resistance gene in feces genome of aquatic products by coupling CuO nanoparticles concatemers-Au@Fe3O4 and 3D DNA walker based on a portable blue light gel imager. Chemical Engineering Journal 2024, 499 , 156311. https://doi.org/10.1016/j.cej.2024.156311
- Qingfeng Yao, Lijuan Liu, Zheng Cai, Mingxia Meng, Shuyue Luo, Jingming Gong. Visual and photoelectrochemical analysis of antibiotic resistance genes enabled by surface-engineered ZIF-8@Au cascade nanozymes. Biosensors and Bioelectronics 2024, 261 , 116470. https://doi.org/10.1016/j.bios.2024.116470
- Yuxin Li, Chengbin Liu, Qiuju Li, Shun Mao. Fluorescence analysis of antibiotics and antibiotic-resistance genes in the environment: A mini review. Chinese Chemical Letters 2024, 35
(10)
, 109541. https://doi.org/10.1016/j.cclet.2024.109541
- Jiawen Xiang, Ji Qi, Die Hu, Chao Wang, Liyan Wang, Yixuan Wu, Jiadong Chen, Zhiyang Zhang, Xiaoyan Wang, Bowei Li, Lingxin Chen. Molecularly imprinted metal-organic frameworks assisted cloth and paper hybrid microfluidic devices for visual detection of gonyautoxin. Journal of Hazardous Materials 2024, 469 , 133969. https://doi.org/10.1016/j.jhazmat.2024.133969
- Yan Ji, Xiuling Jiang, Dongyan Wang. Detection of antibiotic resistance mechanism and research on new anti-infection measures based on big data analysis. Applied Mathematics and Nonlinear Sciences 2024, 9
(1)
https://doi.org/10.2478/amns-2024-0960
- Xinyu Liao, Ruijie Deng, Keith Warriner, Tian Ding. Antibiotic resistance mechanism and diagnosis of common foodborne pathogens based on genotypic and phenotypic biomarkers. Comprehensive Reviews in Food Science and Food Safety 2023, 22
(4)
, 3212-3253. https://doi.org/10.1111/1541-4337.13181
- Lauro A. Pradela-Filho, William B. Veloso, Iana V. S. Arantes, Juliana L. M. Gongoni, Davi M. de Farias, Diele A. G. Araujo, Thiago R. L. C. Paixão. Paper-based analytical devices for point-of-need applications. Microchimica Acta 2023, 190
(5)
https://doi.org/10.1007/s00604-023-05764-5
- Debayan Das, Manaswini Masetty, Aashish Priye. Paper-Based Loop Mediated Isothermal Amplification (LAMP) Platforms: Integrating the Versatility of Paper Microfluidics with Accuracy of Nucleic Acid Amplification Tests. Chemosensors 2023, 11
(3)
, 163. https://doi.org/10.3390/chemosensors11030163
- Bin Xiao, Ruiming Zhao, Nan Wang, Juan Zhang, Xiaoyun Sun, Ailiang Chen. Recent advances in centrifugal microfluidic chip-based loop-mediated isothermal amplification. TrAC Trends in Analytical Chemistry 2023, 158 , 116836. https://doi.org/10.1016/j.trac.2022.116836
- Kieu The Loan Trinh, Woo Ri Chae, Nae Yoon Lee. Recent advances in the fabrication strategies of paper-based microfluidic devices for rapid detection of bacteria and viruses. Microchemical Journal 2022, 180 , 107548. https://doi.org/10.1016/j.microc.2022.107548
- Hao Wang, Chenhao Jia, Hongzhao Li, Rui Yin, Jiang Chen, Yan Li, Min Yue. Paving the way for precise diagnostics of antimicrobial resistant bacteria. Frontiers in Molecular Biosciences 2022, 9 https://doi.org/10.3389/fmolb.2022.976705
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- Marylyn Setsuko Arai, Andrea Simone Stucchi de Camargo, Emanuel Carrilho. Fluorescent paper-based analytical devices. 2022, 183-212. https://doi.org/10.1016/B978-0-12-820534-1.00002-5
- Akkapol Suea‐Ngam, Ilada Choopara, Shangkun Li, Mathias Schmelcher, Naraporn Somboonna, Philip D. Howes, Andrew J. deMello. In Situ Nucleic Acid Amplification and Ultrasensitive Colorimetric Readout in a Paper‐Based Analytical Device Using Silver Nanoplates. Advanced Healthcare Materials 2021, 10
(7)
https://doi.org/10.1002/adhm.202001755
- Cailing Zhou, Yuwei Pan, Shifu Ge, Frederic Coulon, Zhugen Yang. Rapid methods for antimicrobial resistance diagnosis in contaminated soils for effective remediation strategy. TrAC Trends in Analytical Chemistry 2021, 137 , 116203. https://doi.org/10.1016/j.trac.2021.116203
- Junjie Chi, Xiaoxuan Zhang, Yuetong Wang, Changmin Shao, Luoran Shang, Yuanjin Zhao. Bio-inspired wettability patterns for biomedical applications. Materials Horizons 2021, 8
(1)
, 124-144. https://doi.org/10.1039/D0MH01293A
- Guoying Hao, Zhong Zhang, Xin Ma, Runguang Zhang, Xiaoxiao Qin, Hongxia Sun, Xingbin Yang, Jianhui Rong. A versatile microfluidic paper chip platform based on MIPs for rapid ratiometric sensing of dual fluorescence signals. Microchemical Journal 2020, 157 , 105050. https://doi.org/10.1016/j.microc.2020.105050
- Xiaoyan He, Huimin Han, Wenyu Shi, Jiandi Dong, Xiong Lu, Wu Yang, Xiaoquan Lu. A label-free electrochemical DNA biosensor for kanamycin detection based on diblock DNA with poly-cytosine as a high affinity anchor on graphene oxide. Analytical Methods 2020, 12
(27)
, 3462-3469. https://doi.org/10.1039/D0AY00025F
- Kang Mao, Xiaocui Min, Hua Zhang, Kuankuan Zhang, Haorui Cao, Yongkun Guo, Zhugen Yang. Paper-based microfluidics for rapid diagnostics and drug delivery. Journal of Controlled Release 2020, 322 , 187-199. https://doi.org/10.1016/j.jconrel.2020.03.010
- Yuting Shang, Jiadi Sun, Yongli Ye, Jumei Zhang, Yinzhi Zhang, Xiulan Sun. Loop-mediated isothermal amplification-based microfluidic chip for pathogen detection. Critical Reviews in Food Science and Nutrition 2020, 60
(2)
, 201-224. https://doi.org/10.1080/10408398.2018.1518897
- Rajwinder Kaur, Bhoomika Yadav, R.D. Tyagi. Microbiology of hospital wastewater. 2020, 103-148. https://doi.org/10.1016/B978-0-12-819722-6.00004-3
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- Hosub Lim, Ali Turab Jafry, Jinkee Lee. Fabrication, Flow Control, and Applications of Microfluidic Paper-Based Analytical Devices. Molecules 2019, 24
(16)
, 2869. https://doi.org/10.3390/molecules24162869
- Jinjin Shen, Ting Zhou, Ru Huang. Recent Advances in Electrochemiluminescence Sensors for Pathogenic Bacteria Detection. Micromachines 2019, 10
(8)
, 532. https://doi.org/10.3390/mi10080532
- Liping Li, Yimeng Geng, Yuhong Xiang, Hong Qiang, Yuxian Wang, Jianqiao Chang, Hanni Zhao, Lu Zhang. Instrument-free enrichment and detection of phosphopeptides using paper-based Phos-PAD. Analytica Chimica Acta 2019, 1062 , 102-109. https://doi.org/10.1016/j.aca.2019.02.019
- Jing Dong, Qinfeng Xu, Chen-chen Li, Chun-yang Zhang. Single-color multiplexing by the integration of high-resolution melting pattern recognition with loop-mediated isothermal amplification. Chemical Communications 2019, 55
(17)
, 2457-2460. https://doi.org/10.1039/C8CC09741K
- Charu Agarwal, Levente Csóka. Recent Advances in Paper-Based Analytical Devices: A Pivotal Step Forward in Building Next-Generation Sensor Technology. 2019, 479-517. https://doi.org/10.1007/978-3-030-05399-4_18
- Xukui Liu, Xuanhao Zhao, Yumei Li, Kangdi Zheng, Qiong Wu, Wenjie Mei. Microwave-Assisted Synthesis, Characterisation, and DNA-Binding Properties of RuII Complexes Coordinated by Norfloxacin as Potential Tumour Inhibitors. Australian Journal of Chemistry 2019, 72
(5)
, 400. https://doi.org/10.1071/CH18637
- Tiffany-Heather Ulep, Jeong-Yeol Yoon. Challenges in paper-based fluorogenic optical sensing with smartphones. Nano Convergence 2018, 5
(1)
https://doi.org/10.1186/s40580-018-0146-1
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(26)
, 6779-6785. https://doi.org/10.1007/s00216-018-1275-4
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(16)
, 1895-1901. https://doi.org/10.1039/C8AY00372F
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