Vancomycin- and Strontium-Loaded Microspheres with Multifunctional Activities against Bacteria, in Angiogenesis, and in Osteogenesis for Enhancing Infected Bone Regeneration
- Pengfei WeiPengfei WeiState Key Laboratory of Organic−Inorganic Composites, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, P. R. ChinaMore by Pengfei Wei,
- Wei JingWei JingState Key Laboratory of Organic−Inorganic Composites, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, P. R. ChinaMore by Wei Jing,
- Zuoying YuanZuoying YuanState Key Laboratory of Organic−Inorganic Composites, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, P. R. ChinaMore by Zuoying Yuan,
- Yiqian HuangYiqian HuangState Key Laboratory of Organic−Inorganic Composites, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, P. R. ChinaMore by Yiqian Huang,
- Binbin GuanBinbin GuanDepartment of Stomatology, Tianjin Medical University General Hospital, Tianjin 300052, P. R. ChinaMore by Binbin Guan,
- Wenxin ZhangWenxin ZhangDepartment of Endodontics, School and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, P. R. ChinaMore by Wenxin Zhang,
- Xu ZhangXu ZhangDepartment of Endodontics, School and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, P. R. ChinaMore by Xu Zhang,
- Jianping MaoJianping MaoDepartment of Spine Surgery, Beijing Jishuitan Hospital, Beijing 100035, P. R. ChinaMore by Jianping Mao,
- Qing Cai*Qing Cai*E-mail: [email protected] (Q.C.).State Key Laboratory of Organic−Inorganic Composites, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, P. R. ChinaMore by Qing Cai,
- Dafu Chen*Dafu Chen*E-mail: [email protected] (D.C.).Laboratory of Bone Tissue Engineering, Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Beijing 100035, P. R. ChinaMore by Dafu Chen, and
- Xiaoping YangXiaoping YangState Key Laboratory of Organic−Inorganic Composites, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, P. R. ChinaMore by Xiaoping Yang
Abstract
Biomaterials that have capacities to simultaneously induce bone regeneration and kill bacteria are in demand because bone defects face risks of severe infection in clinical therapy. To meet the demand, multifunctional biodegradable microspheres are fabricated, which contain vancomycin to provide antibacterial activity and strontium-doped apatite to provide osteocompatibility. Moreover, the strontium component shows activity in promoting angiogenesis, which further favors osteogenesis. For producing the microspheres, vancomycin is loaded into mesoporous silica and embedded in polylactide-based microspheres via the double emulsion technique and the strontium-doped apatite is deposited onto the microspheres via biomineralization in strontium-containing simulated body fluid. Sustained release behaviors of both vancomycin and Sr2+ ions are achieved. The microspheres exhibit strong antibacterial effect against Staphylococcus aureus, while demonstrating excellent cell/tissue compatibility. Studies of differentiation confirm that the introduction of strontium element strengthens the angiogenic and osteogenic expressions of mesenchymal stromal cells. Subcutaneous injection of the microspheres into rabbit’s back confirms their effectiveness in inducing neovascularization and ectopic osteogenesis. Finally, an infected rabbit femoral condyle defect model is created with S. aureus infection and the multifunctional microspheres are injected, which display significant antibacterial activity in vivo and achieve efficient new bone formation in comparison with biomineralized microspheres without vancomycin loading. The vancomycin- and strontium-loaded microspheres, being biomineralized, injectable, and biodegradable, are attractive because of their flexibility in integrating multiple functions into one design, whose potentials in treating infected bone defects are highly expected.
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This article is cited by 3 publications.
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