Erythrocyte-Derived Optical Nanoprobes Doped with Indocyanine Green-Bound Albumin: Material Characteristics and Evaluation for Cancer Cell ImagingClick to copy article linkArticle link copied!
- Jenny T. MacJenny T. MacDepartment of Biochemistry, University of California, Riverside, California 92521, United StatesMore by Jenny T. Mac
- Raviraj VankayalaRaviraj VankayalaDepartment of Bioengineering, University of California, Riverside, California 92521, United StatesMore by Raviraj Vankayala
- Dipti K. PatelDipti K. PatelDepartment of Bioengineering, University of California, Riverside, California 92521, United StatesMore by Dipti K. Patel
- Sabrina WuesteSabrina WuesteDepartment of Bioengineering, University of California, Riverside, California 92521, United StatesMore by Sabrina Wueste
- Bahman Anvari*Bahman Anvari*E-mail: [email protected]Department of Biochemistry and Department of Bioengineering, University of California, Riverside, California 92521, United StatesMore by Bahman Anvari
Abstract
Nanosize structures activated by near-infrared (NIR) photoexcitation can provide an optical platform for the image-guided removal of small tumor nodules. We have engineered nanoparticles derived from erythrocytes that can be doped with NIR fluorophore indocyanine green (ICG). We refer to these constructs as NIR erythrocyte-derived transducers (NETs). The objective of this study was to determine if ICG-bound albumin (IbA), as the doping material, could enhance the fluorescence emission of NETs, and evaluate the capability of these nanoprobes in imaging cancer cells. Erythrocytes were isolated from bovine whole blood and depleted of hemoglobin to form erythrocyte ghosts (EGs). EGs were then extruded through nanosize porous membranes in the presence of 10–100 μm ICG or Iba (1:1 molar ratio) to form ICG- or IbA-doped NETs. The resulting nanosize constructs were characterized for their diameters, zeta-potentials, absorption, and fluorescence emission spectra. We used fluorescence microscopic imaging to evaluate the capability of the constructs in imaging SKOV3 ovarian cancer cells. Based on dynamic light-scattering measurements, ICG- and IbA-doped NETs had similar diameter distributions (Z-average diameter of 236 and 238 nm, respectively) in phosphate-buffered saline supplemented with 10% fetal bovine serum, which remained nearly constant over the course of 2 h at 37 °C. Despite a much-lower loading efficiency of IbA (∼0.7–8%) as compared to ICG (10–45%), the integrated normalized fluorescence emission of IbA-NETs was 2- to 6-fold higher than ICG-doped NETs. IbA-NETs also demonstrated an enhanced capability in fluorescence imaging of SKOV3 ovarian cancer cells, and can serve as potentially effective nanoprobes for the fluorescence imaging of cancerous cells.
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This article is cited by 7 publications.
- Pei-Yao Xu, Xiang Zheng, Ranjith Kumar Kankala, Shi-Bin Wang, Ai-Zheng Chen. Advances in Indocyanine Green-Based Codelivery Nanoplatforms for Combinatorial Therapy. ACS Biomaterials Science & Engineering 2021, 7
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, 939-962. https://doi.org/10.1021/acsbiomaterials.0c01644
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- Chaolong Liu, Jianping Liu, Hua Ma, Wenzhu Zhang, Bo Song, Lianying Guo, Run Zhang, Jingli Yuan. A folic acid-functionalized dual-emissive nanoprobe for “double-check” luminescence imaging of cancer cells. Methods 2019, 168 , 102-108. https://doi.org/10.1016/j.ymeth.2019.07.001
- Hainan Yang, Zaijia Liu, Xufeng Li, Zhenfeng Zhang, Deji Chen, Hui Lian. Artesunate-Loaded and Near-Infrared Dye-Conjugated Albumin Nanoparticles as High-Efficiency Tumor-Targeted Photo-Chemo Theranostic Agent. Nanoscale Research Letters 2018, 13
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https://doi.org/10.1186/s11671-018-2700-5
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