Sardine Roe as a Source of Lipids To Produce Liposomes
- Marta GuedesMarta Guedes3B’s Research Group, I3Bs − Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, PortugalICVS/3B’s - PT Government Associate Laboratory, Braga, Guimarães 4805-017, PortugalMore by Marta Guedes,
- Ana R. Costa-PintoAna R. Costa-PintoUniversidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina − Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquiteto Lobão Vital 172, 4200-374 Porto, PortugalMore by Ana R. Costa-Pinto,
- Virgínia M. F. GonçalvesVirgínia M. F. GonçalvesCESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Rua Central de Gandra, 1317, 4585-116 Gandra PRD, PortugalMore by Virgínia M. F. Gonçalves,
- Joana Moreira-SilvaJoana Moreira-SilvaInterdisciplinary Centre of Marine and Environmental Research (CIIMAR), University of Porto, Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos, S/N, 4450-208 Matosinhos, PortugalMore by Joana Moreira-Silva,
- Maria Elizabeth TiritanMaria Elizabeth TiritanCESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Rua Central de Gandra, 1317, 4585-116 Gandra PRD, PortugalInterdisciplinary Centre of Marine and Environmental Research (CIIMAR), University of Porto, Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos, S/N, 4450-208 Matosinhos, PortugalLaboratório de Química Orgânica e Farmacêutica, Departamento de Ciências Químicas, Faculdade de Farmácia da Universidade do Porto, Rua Jorge de Viterbo Ferreira 228, 4050-313 Porto, PortugalMore by Maria Elizabeth Tiritan,
- Rui L. ReisRui L. Reis3B’s Research Group, I3Bs − Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, PortugalICVS/3B’s - PT Government Associate Laboratory, Braga, Guimarães 4805-017, PortugalThe Discoveries Centre for Regenerative and Precision Medicine, Headquarters at University of Minho, Avepark, 4805-017 Barco, Guimarães, PortugalMore by Rui L. Reis,
- Helena FerreiraHelena Ferreira3B’s Research Group, I3Bs − Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, PortugalICVS/3B’s - PT Government Associate Laboratory, Braga, Guimarães 4805-017, PortugalMore by Helena Ferreira, and
- Nuno M. Neves*Nuno M. Neves*E-mail: [email protected]3B’s Research Group, I3Bs − Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, PortugalICVS/3B’s - PT Government Associate Laboratory, Braga, Guimarães 4805-017, PortugalThe Discoveries Centre for Regenerative and Precision Medicine, Headquarters at University of Minho, Avepark, 4805-017 Barco, Guimarães, PortugalMore by Nuno M. Neves
Abstract
Sea-derived materials have promising applications in the medical, pharmaceutical, and biotechnological fields. Fish roe, for example, is a highly nutritional product, presenting diverse beneficial effects on human health. Therefore, this work explored extracts of sardine (Sardina pilchardus) roe, due to the well-known health benefits of this fish, to produce novel and promising delivery systems. After morphological, histological, and histochemical characterizations of sardine roe, their lipids were extracted using two different approaches, namely, Bligh and Dyer (BD) and methyl-tert-butyl ether (MTBE) methods. Gas chromatography/mass spectrometry analyses demonstrated that lipid extracts contain several fatty acids, such as ω3 polyunsaturated fatty acids. The lipids, especially phospholipids, were used to produce multilamellar liposomes (MLVs). These delivery systems presented size heterogeneity, a negative surface charge, and the ability to control the release of the encapsulated anti-inflammatory drug, namely, celecoxib. Biological assays indicated that MLVs produced with MTBE lipidic extracts presented a better cytocompatibility than those obtained by the BD method. This can be further improved if the lipid extracts are processed by chemical extraction. Therefore, sardine roe-derived lipids can produce drug-delivery systems with the potential to be applied in the biomedical field.