Low-Intensity Pulsed Ultrasound Responsive Scaffold Promotes Intramembranous and Endochondral Ossification via Ultrasonic, Thermal, and Electrical StimulationClick to copy article linkArticle link copied!
- Wanru JiaWanru JiaDepartment of Ultrasound, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. ChinaCollege of Health Science and Technology, Shanghai Jiao Tong University School of Medicine, No. 227 Chongqing South Road, Shanghai 200025, P.R. ChinaMore by Wanru Jia
- Tianlong WangTianlong WangDepartment of Orthopedics, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. ChinaMore by Tianlong Wang
- Feng Chen*Feng Chen*E-mail: [email protected]Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Shanghai Stomatological Hospital & School of Stomatology, Fudan University, Shanghai 201102, P.R. ChinaMore by Feng Chen
- Zhiqing LiuZhiqing LiuDepartment of Orthopedics, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. ChinaMore by Zhiqing Liu
- Xiaodong HouXiaodong HouDepartment of Orthopedics, First Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming 650032, P.R. ChinaMore by Xiaodong Hou
- Wentao CaoWentao CaoShanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Shanghai Stomatological Hospital & School of Stomatology, Fudan University, Shanghai 201102, P.R. ChinaMore by Wentao Cao
- Xinyu ZhaoXinyu ZhaoDepartment of Orthopedics, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. ChinaMore by Xinyu Zhao
- Bingqiang LuBingqiang LuDepartment of Orthopedics, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. ChinaMore by Bingqiang Lu
- Yan HuYan HuDepartment of Ultrasound, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. ChinaMore by Yan Hu
- Yijie DongYijie DongDepartment of Ultrasound, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. ChinaCollege of Health Science and Technology, Shanghai Jiao Tong University School of Medicine, No. 227 Chongqing South Road, Shanghai 200025, P.R. ChinaMore by Yijie Dong
- Jianqiao Zhou*Jianqiao Zhou*E-mail: [email protected]Department of Ultrasound, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. ChinaCollege of Health Science and Technology, Shanghai Jiao Tong University School of Medicine, No. 227 Chongqing South Road, Shanghai 200025, P.R. ChinaMore by Jianqiao Zhou
- Zifei Zhou*Zifei Zhou*E-mail: [email protected]Department of Orthopedics, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. ChinaMore by Zifei Zhou
- Weiwei Zhan*Weiwei Zhan*E-mail: [email protected]Department of Ultrasound, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. ChinaCollege of Health Science and Technology, Shanghai Jiao Tong University School of Medicine, No. 227 Chongqing South Road, Shanghai 200025, P.R. ChinaMore by Weiwei Zhan
Abstract

Multiple physical stimuli are expected to produce a synergistic effect to promote bone tissue regeneration. Low-intensity pulsed ultrasound (LIPUS) has been clinically used in bone repair for the mechanical stimulation that it provides. In addition, LIPUS can also excite the biomaterials to generate other physical stimuli such as thermal or electrical stimuli. In this study, a scaffold based on decellularized adipose tissue (DAT) is established by incorporating polydopamine-modified multilayer black phosphorus nanosheets (pDA-mBP@DAT). Their effect on bone repair under LIPUS stimulation and the potential mechanisms are further investigated. This scaffold possesses piezoelectric properties and generates a mild thermogenic stimulus when stimulated by LIPUS. With superior properties, this scaffold is demonstrated to have good cytocompatibility in vitro and in vivo. Simultaneously, LIPUS promotes cell attachment, migration, and osteogenic differentiation in the pDA-mBP@DAT scaffold. Furthermore, the combined use of pDA-mBP@DAT and LIPUS significantly affects the regenerative effect in rat models of critical-sized calvarial defects. The possible mechanisms include promoting osteogenesis and neovascularization and activating the Piezo1. This study presents insight into speeding up bone regeneration by the synergistic combination of LIPUS and pDA-mBP@DAT scaffolds.
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