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Gold Nanorod Induced Warming of Embryos from the Cryogenic State Enhances Viability

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† Department of Mechanical Engineering, University of Minnesota at Twin Cities, 111 Church Street SE, Minneapolis, Minnesota 55455, United States
‡ Smithsonian Conservation Biology Institute, Smithsonian National Zoological Park, 3001 Connecticut Avenue NW, Washington, D.C. 20008, United States
§ Hawaii Institute of Marine Biology, University of Hawaii, 46-007 Lilipuna Road, Kaneohe, Hawaii 96744, United States
∥ Department of Biomedical Engineering, University of Minnesota at Twin Cities, 312 Church Street SE, Minneapolis, Minnesota 55455, United States
*Tel: 612-625-5513. E-mail: [email protected]
Cite this: ACS Nano 2017, 11, 8, 7869–7878
Publication Date (Web):July 13, 2017
https://doi.org/10.1021/acsnano.7b02216
Copyright © 2017 American Chemical Society
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Abstract

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Zebrafish embryos can attain a stable cryogenic state by microinjection of cryoprotectants followed by rapid cooling, but the massive size of the embryo has consistently led to failure during the convective warming process. Here we address this zebrafish cryopreservation problem by using gold nanorods (GNRs) to assist in the warming process. Specifically, we microinjected the cryoprotectant propylene glycol into zebrafish embryos along with GNRs, and the samples were cooled at a rate of 90 000 °C/min in liquid nitrogen. We demonstrated the ability to unfreeze the zebrafish rapidly (1.4 × 107 °C/min) by irradiating the sample with a 1064 nm laser pulse for 1 ms due to the excitation of GNRs. This rapid warming process led to the outrunning of ice formation, which can damage the embryos. The results from 14 trials (n = 223) demonstrated viable embryos with consistent structure at 1 h (31%) and continuing development at 3 h (17%) and movement at 24 h (10%) postwarming. This compares starkly with 0% viability, structure, or movement at all time points in convectively warmed controls (n = 50, p < 0.001, ANOVA). Our nanoparticle-based warming process could be applied to the storage of fish, and with proper modification, can potentially be used for other vertebrate embryos.

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The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acsnano.7b02216.

  • More information on the characterization of laser fluence rate and the selection of an appropriate GNR concentration; more details on thermal modeling setup that was used evaluating cooling and warming rates and thermal stress; data to support the success of laser GNR warming to rearm frozen HDF cells; histological images of zebrafish taken 7 days after being injected with GNRs (PDF)

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Cited By

This article is cited by 34 publications.

  1. Kanav Khosla, Li Zhan, Aditya Bhati, Aiden Carley-Clopton, Mary Hagedorn, John Bischof. Characterization of Laser Gold Nanowarming: A Platform for Millimeter-Scale Cryopreservation. Langmuir 2019, 35 (23) , 7364-7375. https://doi.org/10.1021/acs.langmuir.8b03011
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  4. Yilin Liu, Joseph Kangas, Yiru Wang, Kanav Khosla, Jacqueline Pasek-Allen, Aaron Saunders, Steven Oldenburg, John Bischof. Photothermal conversion of gold nanoparticles for uniform pulsed laser warming of vitrified biomaterials. Nanoscale 2020, 12 (23) , 12346-12356. https://doi.org/10.1039/D0NR01614D
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