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Solution Structure and Conformational Changes of the Streptomyces Chitin-Binding Protein (CHB1)
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    Solution Structure and Conformational Changes of the Streptomyces Chitin-Binding Protein (CHB1)
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    European Molecular Biology Laboratory, Hamburg Outstation, EMBL c/o DESY, Notkestrasse 85, D-22603 Hamburg, Germany, Institute of Crystallography, Russian Academy of Sciences, Leninsky pr. 59, 117333 Moscow, Russia, Abteilung Angewandte Genetik der Mikroorganismen, and Abteilung Zoophysiologie, FB Biologie/Chemie, Universität Osnabrück, D-49069 Osnabrück, Germany, and Whitney Laboratory, University of Florida, St. Augustine, Florida 32086
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    Biochemistry

    Cite this: Biochemistry 2000, 39, 35, 10677–10683
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    https://doi.org/10.1021/bi000865p
    Published August 11, 2000
    Copyright © 2000 American Chemical Society

    Abstract

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    The shape and overall dimensions of the recently discovered Streptomyces α-chitin-binding protein, CHB1, were investigated by synchrotron radiation X-ray solution scattering. The radius of gyration and the maximum size of CHB1 were determined to be 1.75 ± 0.03 nm and 6.0 ± 0.2 nm, respectively. Using two independent ab initio approaches the low-resolution shape of the protein was found to consist of two domains, an elongated main globule with a length of about 4 nm and a foot-like domain of about 2 nm width. The structural and functional properties of CHB1 depend strongly on the presence of disulfide bonds; upon their reduction, the protein loses its affinity to chitin.

    Copyright © 2000 American Chemical Society

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     This research was supported by the Deutsche Forschungsgemeinschaft (Schr203/6-2; awarded to H.S.), by the International Association for the Promotion of Cooperation with Scientists from the Independent States of the Former Soviet Union (INTAS; to D.I S. and M.H.J.K.) Grant 96-1115, the EU Biotechnology Program Grant BIO4-CT97-2143 (to D.I.S.). G.G. was supported by the National Institutes of Health (Grant A1 22444).

     European Molecular Biology Laboratory.

    §

     Institute of Crystallography.

     Abteilung Angewandte Genetik der Mikroorganismen.

    *

     To whom correspondence should be addressed. Phone:  +49/(0)541 969 3504. Fax:  +49/(0)541 969 3503. E-mail:  ggrueber@ biologie.uni-osnabrueck.de.

     Abteilung Zoophysiologie.

    #

     University of Florida.

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    Biochemistry

    Cite this: Biochemistry 2000, 39, 35, 10677–10683
    Click to copy citationCitation copied!
    https://doi.org/10.1021/bi000865p
    Published August 11, 2000
    Copyright © 2000 American Chemical Society

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