2'-0-(α-Methoxyethyl)nucleoside 5'-diphosphates as single-addition substrates in the synthesis of specific oligoribonucleotides with polynucleotide phosphorylase

G. N. Bennett, J. K. Mackey, J. L. Wiebers, and P. T. Gilham

Biochemistry, 1973, 12 (20), pp 3956–3962

DOI: 10.1021/bi00744a027

Publication Date: September 1973

Cite this:Biochemistry 12, 20, 3956-3962

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Article

2'-0-(α-Methoxyethyl)nucleoside 5'-diphosphates as single-addition substrates in the synthesis of specific oligoribonucleotides with polynucleotide phosphorylase

Enzymic oligoribonucleotide synthesis with T4 RNA ligase

Activity of polynucleotide phosphorylase with nucleoside diphosphates containing sugar ring modifications

Chemical synthesis of oligonucleotides

Single addition substrates for the synthesis of specific oligoribonucleotides with polynucleotide phosphorylase. Synthesis of 2'-O-(α-methoxyethyl)nucleoside 5'-diphosphates

Stepwise enzymic oligoribonucleotide synthesis including modified nucleotides

Polynucleotides containing hybrid sequences. Synthesis of oligonucleotides possessing a single ribonucleotidyl-(3',5')-deoxyribonucleotide linkage

Stepwise degradation of polyribonucleotides

Polynucleotides containing hybrid sequences. Synthesis of oligonucleotides containing thymidine, adenosine, and a single deoxyribonucleotidyl-(3'-5')-ribonucleotide linkage

Polynucleotide Phosphorylase

Separation of synthetic oligonucleotides on columns of microparticulate silica coated with crosslinked polyethylene imine

New method for the separation of oligonucleotides by chromatography on acriflavin-agarose

17 Polynucleotide Phosphorylase

Enzymatic Synthesis of Polynucleotides of Defined Sequence

Polynucleotides 58 A method for the synthesis of oligonucleotide by single addition of 2′-O-(o-nitrobenzyl)nucleoside 5′-diphosphates using polynucleotide phosphorylase

The Synthesis of Polynucleotides

Participation of isomeric tRNA'S in the partial reactions of protein biosynthesis

Separation of oligonucleotides, nucleotides, and nucleosides on columns of polystyrene anion-exchangers with solvent systems containing ethanol

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2'-0-(α-Methoxyethyl)nucleoside 5'-diphosphates as single-addition substrates in the synthesis of specific oligoribonucleotides with polynucleotide phosphorylase

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