Methods for the Separation of Limonoids from Citrus
- Guddadarangavvanahally K. JayaprakashaGuddadarangavvanahally K. JayaprakashaVegetable and Fruit Improvement Center, Department of Horticultural Sciences, Mail Code 2119, Texas A&M University, College Station, TX 77843
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- Jennifer S. BrodbeltJennifer S. BrodbeltDepartment of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX 78712More by Jennifer S. Brodbelt
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- Narayan G. BhatNarayan G. BhatDepartment of Chemistry, The University of Texas-Pan American, 1201 West University Drive, Edinburg, TX 78539More by Narayan G. Bhat
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- Bhimanagouda S. PatilBhimanagouda S. PatilVegetable and Fruit Improvement Center, Department of Horticultural Sciences, Mail Code 2119, Texas A&M University, College Station, TX 77843More by Bhimanagouda S. Patil
Abstract
Limonoids are naturally occurring triterpenes found in Rutaceae and Meliaceae family plants. Many citrus limonoids have proved to be particularly difficult to purify using conventional methods. This chapter provides an overview of the methods used to extract and purify limonoid aglycones and limonoid glucosides from citrus, including solid liquid extraction, column chromatography, preparative HPLC and flash chromatography. Furthermore, recent literature on analytical methods such as HPLC and LC-MS for the separation and identification of limonoids have been discussed.