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Tailored Design of Au Nanoparticle-siRNA Carriers Utilizing Reversible Addition−Fragmentation Chain Transfer Polymers∥

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Departments of Polymer Science and Chemistry and Biochemistry, The University of Southern Mississippi, Hattiesburg, Mississippi 39406
* To whom correspondence should be addressed. E-mail: [email protected]; [email protected]
∥Paper number 145 in a series entitled “Water-Soluble Polymers”.
†Department of Polymer Science.
‡Department of Chemistry and Biochemistry.
Cite this: Biomacromolecules 2010, 11, 4, 1052–1059
Publication Date (Web):March 25, 2010
https://doi.org/10.1021/bm100020x
Copyright © 2010 American Chemical Society
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The facile synthesis of polymer-stabilized Au nanoparticles (AuNPs) capable of forming neutral, sterically stable complexes with small interfering RNA (siRNA) is reported. The amine-containing cationic block of poly(N-2-hydroxypropyl methacrylamide70-block-N-[3-(dimethylamino)propyl] methacrylamide24) [P(HPMA70-b-DMAPMA24)] was utilized to promote the in situ reduction of Au3+ to AuNPs and subsequently bind small interfering RNA, while the nonimmunogenic, hydrophilic block provided steric stabilization. The ratio of [DMAPMA]0/[Au3+]0 utilized in the reduction reaction was found to be critical to the production of polymer-stabilized AuNPs capable of complexing siRNA. Significant protection (∼100 times) against nucleases was demonstrated by enzymatic tests, while gene down-regulation experiments indicated successful delivery of siRNA to cancerous cells.

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TGA of polymer and polymer-stabilized AuNPs, small-angle X-ray scattering of polymer stabilized AuNPs, and cytotoxicity results. This material is available free of charge via the Internet at http://pubs.acs.org.

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