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Synthesis of Biocompatible PEG-Based Star Polymers with Cationic and Degradable Core for siRNA Delivery

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Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, United States
§ Bone Tissue Engineering Center, Carnegie Mellon University, 5000 Forbes Avenue, Pittsburgh, Pennsylvania 15213, United States
Department of Polymer Science and Engineering, Pusan National University, Busan, South Korea
Cite this: Biomacromolecules 2011, 12, 10, 3478–3486
Publication Date (Web):September 6, 2011
https://doi.org/10.1021/bm2006455
Copyright © 2011 American Chemical Society

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    Abstract

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    Star polymers with poly(ethylene glycol) (PEG) arms and a degradable cationic core were synthesized by the atom transfer radical copolymerization (ATRP) of poly(ethylene glycol) methyl ether methacrylate macromonomer (PEGMA), 2-(dimethylamino)ethyl methacrylate (DMAEMA), and a disulfide dimethacrylate (cross-linker, SS) via an “arm-first” approach. The star polymers had a diameter ∼15 nm and were degraded under redox conditions by glutathione treatment into individual polymeric chains due to cleavage of the disulfide cross-linker, as confirmed by dynamic light scattering. The star polymers were cultured with mouse calvarial preosteoblast-like cells, embryonic day 1, subclone 4 (MC3T3-E1.4) to determine biocompatibility. Data suggest star polymers were biocompatible, with ≥80% cell viability after 48 h of incubation even at high concentration (800 μg/mL). Zeta potential values varied with N/P ratio confirming complexation with siRNA. Successful cellular uptake of the star polymers in MC3T3-E1.4 cells was observed by confocal microscopy and flow cytometry after 24 h of incubation.

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    12. Jana Herzberger, Kerstin Niederer, Hannah Pohlit, Jan Seiwert, Matthias Worm, Frederik R. Wurm, and Holger Frey . Polymerization of Ethylene Oxide, Propylene Oxide, and Other Alkylene Oxides: Synthesis, Novel Polymer Architectures, and Bioconjugation. Chemical Reviews 2016, 116 (4) , 2170-2243. https://doi.org/10.1021/acs.chemrev.5b00441
    13. Vanessa Loczenski Rose, Saif Shubber, S. Sajeesh, Sebastian G. Spain, Sanyogitta Puri, Stephanie Allen, Dong-Ki Lee, G. Sebastiaan Winkler, and Giuseppe Mantovani . Phosphonium Polymethacrylates for Short Interfering RNA Delivery: Effect of Polymer and RNA Structural Parameters on Polyplex Assembly and Gene Knockdown. Biomacromolecules 2015, 16 (11) , 3480-3490. https://doi.org/10.1021/acs.biomac.5b00898
    14. Barbara Mendrek, Łukasz Sieroń, Iwona Żymełka-Miara, Paulina Binkiewicz, Marcin Libera, Mario Smet, Barbara Trzebicka, Aleksander L. Sieroń, Agnieszka Kowalczuk, and Andrzej Dworak . Nonviral Plasmid DNA Carriers Based on N,N′-Dimethylaminoethyl Methacrylate and Di(ethylene glycol) Methyl Ether Methacrylate Star Copolymers. Biomacromolecules 2015, 16 (10) , 3275-3285. https://doi.org/10.1021/acs.biomac.5b00948
    15. Takaya Terashima, Saki Nishioka, Yuta Koda, Mikihito Takenaka, and Mitsuo Sawamoto . Arm-Cleavable Microgel Star Polymers: A Versatile Strategy for Direct Core Analysis and Functionalization. Journal of the American Chemical Society 2014, 136 (29) , 10254-10257. https://doi.org/10.1021/ja505646p
    16. Patrícia V. Mendonça, Saadyah E. Averick, Dominik Konkolewicz, Arménio C. Serra, Anatoliy V. Popov, Tamaz Guliashvili, Krzysztof Matyjaszewski, and Jorge F. J. Coelho . Straightforward ARGET ATRP for the Synthesis of Primary Amine Polymethacrylate with Improved Chain-End Functionality under Mild Reaction Conditions. Macromolecules 2014, 47 (14) , 4615-4621. https://doi.org/10.1021/ma501007j
    17. Joshua Lehr, Flávio C. Bedatty Fernandes, Paulo R. Bueno, and Jason J. Davis . Label-free Capacitive Diagnostics: Exploiting Local Redox Probe State Occupancy. Analytical Chemistry 2014, 86 (5) , 2559-2564. https://doi.org/10.1021/ac403727h
    18. Takaya Terashima, Takanori Sugita, Kaoru Fukae, and Mitsuo Sawamoto . Synthesis and Single-Chain Folding of Amphiphilic Random Copolymers in Water. Macromolecules 2014, 47 (2) , 589-600. https://doi.org/10.1021/ma402355v
    19. Antons Sizovs, Xianzhou Song, M. Neal Waxham, Yilong Jia, Fude Feng, Jianwei Chen, Amanda C. Wicker, Jianming Xu, Yan Yu, and Jin Wang . Precisely Tunable Engineering of Sub-30 nm Monodisperse Oligonucleotide Nanoparticles. Journal of the American Chemical Society 2014, 136 (1) , 234-240. https://doi.org/10.1021/ja408879b
    20. Saadyah E. Averick, Eduardo Paredes, Sourav K. Dey, Kristin M. Snyder, Nikos Tapinos, Krzysztof Matyjaszewski, and Subha R. Das . Autotransfecting Short Interfering RNA through Facile Covalent Polymer Escorts. Journal of the American Chemical Society 2013, 135 (34) , 12508-12511. https://doi.org/10.1021/ja404520j
    21. Sangwoo Park, Hong Yul Cho, Katarzyna Barbara Wegner, Joanna Burdynska, Andrew J. D. Magenau, Hyun-jong Paik, Stefan Jurga, and Krzysztof Matyjaszewski . Star Synthesis Using Macroinitiators via Electrochemically Mediated Atom Transfer Radical Polymerization. Macromolecules 2013, 46 (15) , 5856-5860. https://doi.org/10.1021/ma401308e
    22. Cyrille Boyer, Joann Teo, Phoebe Phillips, Rafael B. Erlich, Sharon Sagnella, George Sharbeen, Tanya Dwarte, Hien T. T. Duong, David Goldstein, Thomas P. Davis, Maria Kavallaris, and Joshua McCarroll . Effective Delivery of siRNA into Cancer Cells and Tumors Using Well-Defined Biodegradable Cationic Star Polymers. Molecular Pharmaceutics 2013, 10 (6) , 2435-2444. https://doi.org/10.1021/mp400049e
    23. Hong Y. Cho, Saadyah E. Averick, Eduardo Paredes, Katarzyna Wegner, Amram Averick, Stefan Jurga, Subha R. Das, and Krzysztof Matyjaszewski . Star Polymers with a Cationic Core Prepared by ATRP for Cellular Nucleic Acids Delivery. Biomacromolecules 2013, 14 (5) , 1262-1267. https://doi.org/10.1021/bm4003199
    24. Abhijeet P. Bapat, Jacob G. Ray, Daniel A. Savin, and Brent S. Sumerlin . Redox-Responsive Dynamic-Covalent Assemblies: Stars and Miktoarm Stars. Macromolecules 2013, 46 (6) , 2188-2198. https://doi.org/10.1021/ma400169m
    25. Hyungsuk Lim, Joungyoun Noh, Yerang Kim, Hyungmin Kim, Jihye Kim, Gilson Khang, and Dongwon Lee . Acid-Degradable Cationic Poly(ketal amidoamine) for Enhanced RNA Interference In Vitro and In Vivo. Biomacromolecules 2013, 14 (1) , 240-247. https://doi.org/10.1021/bm301669e
    26. Saadyah E. Averick, Eduardo Paredes, Ainara Irastorza, Arun R. Shrivats, Abiraman Srinivasan, Daniel J. Siegwart, Andrew J. Magenau, Hong Y. Cho, Eric Hsu, Amram A. Averick, Jinku Kim, Shiguang Liu, Jeffrey O. Hollinger, Subha R. Das, and Krzysztof Matyjaszewski . Preparation of Cationic Nanogels for Nucleic Acid Delivery. Biomacromolecules 2012, 13 (11) , 3445-3449. https://doi.org/10.1021/bm301166s
    27. Daniel J. Phillips and Matthew I. Gibson . Biodegradable Poly(disulfide)s Derived from RAFT Polymerization: Monomer Scope, Glutathione Degradation, and Tunable Thermal Responses. Biomacromolecules 2012, 13 (10) , 3200-3208. https://doi.org/10.1021/bm300989s
    28. Kun Huang, Mark Johnson, and Javid Rzayev . Synthesis of Degradable Organic Nanotubes by Bottlebrush Molecular Templating. ACS Macro Letters 2012, 1 (7) , 892-895. https://doi.org/10.1021/mz3002287
    29. Krzysztof Matyjaszewski. Current status and outlook for ATRP. European Polymer Journal 2024, 211 , 113001. https://doi.org/10.1016/j.eurpolymj.2024.113001
    30. Reihaneh Farajollah, Mir Mohammad Alavi Nikje, Farid Abedin Dorkoosh. Hierarchical porous bio polyurethane film as an implantable delivery system for sustained release of insulin: Synthesis, characterization, and in-vitro evaluation. Reactive and Functional Polymers 2024, 197 , 105860. https://doi.org/10.1016/j.reactfunctpolym.2024.105860
    31. Chengbin Yang, Zheng‐Ian Lin, Xinmeng Zhang, Zhourui Xu, Gaixia Xu, Yu‐Min Wang, Tzu‐Hsien Tsai, Pei‐Wen Cheng, Wing‐Cheung Law, Ken‐Tye Yong, Chih‐Kuang Chen. Recent Advances in Engineering Carriers for siRNA Delivery. Macromolecular Bioscience 2024, 24 (4) https://doi.org/10.1002/mabi.202300362
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    34. Yi Wang, Francesca Lorandi, Marco Fantin, Krzysztof Matyjaszewski. Atom transfer radical polymerization in dispersed media with low-ppm catalyst loading. Polymer 2023, 275 , 125913. https://doi.org/10.1016/j.polymer.2023.125913
    35. Zhili Li, Haiyang Yong, Kaixuan Wang, Ya-Nan Zhou, Jing Lyu, Lirong Liang, Dezhong Zhou. (Controlled) Free radical (co)polymerization of multivinyl monomers: strategies, topological structures and biomedical applications. Chemical Communications 2023, 59 (28) , 4142-4157. https://doi.org/10.1039/D3CC00250K
    36. Antonina Simakova, Saadyah Averick, Arman Moini Jazani, Krzysztof Matyjaszewski. Controlling Size and Surface Chemistry of Cationic Nanogels by Inverse Microemulsion ATRP. Macromolecular Chemistry and Physics 2023, 224 (1) , 2200210. https://doi.org/10.1002/macp.202200210
    37. Jose Caceres-Alban, Midori Sanchez, Fanny L. Casado. Bioprinting: A Strategy to Build Informative Models of Exposure and Disease. IEEE Reviews in Biomedical Engineering 2023, 16 , 594-610. https://doi.org/10.1109/RBME.2022.3146293
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    48. Nathaniel Corrigan, Kenward Jung, Graeme Moad, Craig J. Hawker, Krzysztof Matyjaszewski, Cyrille Boyer. Reversible-deactivation radical polymerization (Controlled/living radical polymerization): From discovery to materials design and applications. Progress in Polymer Science 2020, 111 , 101311. https://doi.org/10.1016/j.progpolymsci.2020.101311
    49. Nirmal Marasini, Changkui Fu, Nicholas L. Fletcher, Christopher Subasic, Gerald Er, Karine Mardon, Kristofer J. Thurecht, Andrew K. Whittaker, Lisa M. Kaminskas. The Impact of Polymer Size and Cleavability on the Intravenous Pharmacokinetics of PEG-Based Hyperbranched Polymers in Rats. Nanomaterials 2020, 10 (12) , 2452. https://doi.org/10.3390/nano10122452
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    51. Yongsheng Gao, Dezhong Zhou, Jing Lyu, Sigen A, Qian Xu, Ben Newland, Krzysztof Matyjaszewski, Hongyun Tai, Wenxin Wang. Complex polymer architectures through free-radical polymerization of multivinyl monomers. Nature Reviews Chemistry 2020, 4 (4) , 194-212. https://doi.org/10.1038/s41570-020-0170-7
    52. Y. Zhao, C. Simon, M. Daoud Attieh, K. Haupt, A. Falcimaigne-Cordin. Reduction-responsive molecularly imprinted nanogels for drug delivery applications. RSC Advances 2020, 10 (10) , 5978-5987. https://doi.org/10.1039/C9RA07512G
    53. Thomas J. Gibson, Peter Smyth, Mona Semsarilar, Aidan P. McCann, William J. McDaid, Michael C. Johnston, Christopher J. Scott, Efrosyni Themistou. Star polymers with acid-labile diacetal-based cores synthesized by aqueous RAFT polymerization for intracellular DNA delivery. Polymer Chemistry 2020, 11 (2) , 344-357. https://doi.org/10.1039/C9PY00573K
    54. Saiful Hoque, Valliappan Raju. Influence of Utilitarian Shopping and Brand Orientation on Online Purchase Intention via Trust. A Testing of SOR Model. Global Journal Of Emerging Sciences 2020, , 70-84. https://doi.org/10.37187/gjoes.0320.0201.07
    55. Alexander B. Cook, Sébastien Perrier. Branched and Dendritic Polymer Architectures: Functional Nanomaterials for Therapeutic Delivery. Advanced Functional Materials 2020, 30 (2) https://doi.org/10.1002/adfm.201901001
    56. Mohammad Amir Qureshi, Fehmeeda Khatoon. Different types of smart nanogel for targeted delivery. Journal of Science: Advanced Materials and Devices 2019, 4 (2) , 201-212. https://doi.org/10.1016/j.jsamd.2019.04.004
    57. Jeroen H. Vrijsen, Camila Osiro Medeiros, Jonas Gruber, Tanja Junkers. Continuous flow synthesis of core cross-linked star polymers via photo-induced copper mediated polymerization. Polymer Chemistry 2019, 10 (13) , 1591-1598. https://doi.org/10.1039/C9PY00134D
    58. Sepideh Khoee, Shahnaz Rahimi. Reversible core–shell crosslinked micelles for controlled release of bioactive agents. 2019, 119-167. https://doi.org/10.1016/B978-0-12-816200-2.00016-5
    59. Hung-Hsun Lu, Cheng-Hung Huang, Ting-Yun Shiue, Fu-Sheng Wang, Ko-Kai Chang, Yunching Chen, Chi-How Peng. Highly efficient gene release in spatiotemporal precision approached by light and pH dual responsive copolymers. Chemical Science 2019, 10 (1) , 284-292. https://doi.org/10.1039/C8SC01494A
    60. Barbara Mendrek, Agnieszka Fus, Katarzyna Klarzyńska, Aleksander Sieroń, Mario Smet, Agnieszka Kowalczuk, Andrzej Dworak. Synthesis, Characterization and Cytotoxicity of Novel Thermoresponsive Star Copolymers of N,N′-Dimethylaminoethyl Methacrylate and Hydroxyl-Bearing Oligo(Ethylene Glycol) Methacrylate. Polymers 2018, 10 (11) , 1255. https://doi.org/10.3390/polym10111255
    61. Udayan Basak, Radhakanta Ghosh, Tapas Ghosh, Soumabrata Majumdar, Mahuya Pakhira, Tuhin Ghosh, Dhruba P. Chatterjee. Synthesis of ‘living’ poly(2-dimethylaminoethyl methacrylate) and stimuli responsive/multifunctional block copolymers effective in fabrication of CdS ‘smart’ ‘Q-Particles’. Polymer 2018, 155 , 27-41. https://doi.org/10.1016/j.polymer.2018.09.013
    62. Qijing Chen, Fei Han, Chao Lin, Xuejun Wen, Peng Zhao. Synthesis of bioreducible core crosslinked star polymers with N,N′-bis(acryloyl)cystamine crosslinker via aqueous ethanol dispersion RAFT polymerization. Polymer 2018, 146 , 378-385. https://doi.org/10.1016/j.polymer.2018.05.058
    63. Marilene Estanqueiro, Helena Vasconcelos, José Manuel Sousa Lobo, Helena Amaral. Delivering miRNA modulators for cancer treatment. 2018, 517-565. https://doi.org/10.1016/B978-0-12-813689-8.00014-8
    64. Jing Zhang, Qingzhong Zhang, Shuaifeng Zhou, Yuping Liu, Wei Huang. Synthesis and characterization of amphiphilic miktoarm star polymers based on sydnone-maleimide double cycloaddition. Polymer Chemistry 2018, 9 (2) , 203-212. https://doi.org/10.1039/C7PY01476G
    65. Shahed Behzadi, Jochen Stadler, Saman Hosseinpour, Daniel Crespy, Katharina Landfester. Suppressing non-controlled leakage of hydrophilic payloads from redox-responsive nanocapsules. Colloids and Surfaces A: Physicochemical and Engineering Aspects 2017, 532 , 2-7. https://doi.org/10.1016/j.colsurfa.2017.07.077
    66. Stanislav Rangelov, Stergios Pispas. Nanoparticles: Biological Applications. 2017, 1109-1169. https://doi.org/10.1081/E-EBPPC-120052544
    67. Venkatanarasimhan Swarnalatha, Mukundamurthy Kannan, Raghavachari Dhamodharan. Atom Transfer Radical Polymerization: A Key Tool Towards the Design and Synthesis of Functional Polymers. 2017, 57-126. https://doi.org/10.1201/9781315366524-4
    68. Xiangang Hu, Anqi Sun, Weilu Kang, Qixing Zhou. Strategies and knowledge gaps for improving nanomaterial biocompatibility. Environment International 2017, 102 , 177-189. https://doi.org/10.1016/j.envint.2017.03.001
    69. Xin Liao, Grace Walden, Noelia D. Falcon, Simon Donell, Michael J. Raxworthy, Michael Wormstone, Graham P. Riley, Aram Saeed. A direct comparison of linear and star-shaped poly(dimethylaminoethyl acrylate) polymers for polyplexation with DNA and cytotoxicity in cultured cell lines. European Polymer Journal 2017, 87 , 458-467. https://doi.org/10.1016/j.eurpolymj.2016.08.021
    70. Vaidas Klimkevicius, Ricardas Makuska. Successive RAFT polymerization of poly(ethylene oxide) methyl ether methacrylates with different length of PEO chains giving diblock brush copolymers. European Polymer Journal 2017, 86 , 94-105. https://doi.org/10.1016/j.eurpolymj.2016.11.026
    71. Jinming Hu, Ruirui Qiao, Michael R. Whittaker, John F. Quinn, Thomas P. Davis. Synthesis of Star Polymers by RAFT Polymerization as Versatile Nanoparticles for Biomedical Applications. Australian Journal of Chemistry 2017, 70 (11) , 1161. https://doi.org/10.1071/CH17391
    72. Christian P. E. Guyader, Baptiste Lamarre, Emiliana De Santis, James E. Noble, Nigel K. Slater, Maxim G. Ryadnov. Autonomously folded α-helical lockers promote RNAi*. Scientific Reports 2016, 6 (1) https://doi.org/10.1038/srep35012
    73. Roshan James, Ohan S. Manoukian, Sangamesh G. Kumbar. Poly(lactic acid) for delivery of bioactive macromolecules. Advanced Drug Delivery Reviews 2016, 107 , 277-288. https://doi.org/10.1016/j.addr.2016.06.009
    74. Xiaobo Zhu, Michael Fryd, Carlos Barrero, Salim Merali, Chiara Fecchio, Ann M. Valentine, Bradford B. Wayland. Kinetic-mechanistic studies of P. cepacia lipase catalyzed corona charge selective micelle degradation. Journal of Molecular Catalysis B: Enzymatic 2016, 133 , 187-195. https://doi.org/10.1016/j.molcatb.2016.08.013
    75. Kyobum Kim, William C.W. Chen, Yunhoe Heo, Yadong Wang. Polycations and their biomedical applications. Progress in Polymer Science 2016, 60 , 18-50. https://doi.org/10.1016/j.progpolymsci.2016.05.004
    76. Shihai Wang, Yu Zhou, Bo Zhuang, Peng Zheng, Hongxiang Chen, Tongcun Zhang, Haiman Hu, Dengcheng Huang. Star‐shaped amphiphilic block polyurethane with pentaerythritol core for a hydrophobic drug delivery carrier. Polymer International 2016, 65 (5) , 551-558. https://doi.org/10.1002/pi.5092
    77. Mengtian Leng, Shaodong Hu, Aijing Lu, Mengtan Cai, Xianglin Luo. The anti-bacterial poly(caprolactone)-poly(quaternary ammonium salt) as drug delivery carriers. Applied Microbiology and Biotechnology 2016, 100 (7) , 3049-3059. https://doi.org/10.1007/s00253-015-7126-8
    78. Xilin Bai, Suying Xu, Jiali Liu, Leyu Wang. Upconversion luminescence tracking of gene delivery via multifunctional nanocapsules. Talanta 2016, 150 , 118-124. https://doi.org/10.1016/j.talanta.2015.08.038
    79. Fiona O’Neill. Resistance of Brain Tumours to Small-Molecule-Targeted Therapies: Lessons from Various Cancer Types. 2016, 89-105. https://doi.org/10.1007/978-3-319-46505-0_5
    80. G. Kapiti, H. Keul, M. Möller. Functional PEG building blocks via copolymerization of ethylene carbonate and tert-butyl glycidyl ether. Polymer Chemistry 2016, 7 (31) , 5050-5059. https://doi.org/10.1039/C6PY00768F
    81. Stanislav Rangelov, Stergios Pispas. Nanoparticles: Biological Applications. 2015, 5437-5497. https://doi.org/10.1081/E-EBPP-120052544
    82. Vianney Delplace, Julien Nicolas. Degradable vinyl polymers for biomedical applications. Nature Chemistry 2015, 7 (10) , 771-784. https://doi.org/10.1038/nchem.2343
    83. Wei Wu, Weigang Wang, Jianshu Li. Star polymers: Advances in biomedical applications. Progress in Polymer Science 2015, 46 , 55-85. https://doi.org/10.1016/j.progpolymsci.2015.02.002
    84. Huiyuan Wang, Yifan Jiang, Huige Peng, Yingzhi Chen, Peizhi Zhu, Yongzhuo Huang. Recent progress in microRNA delivery for cancer therapy by non-viral synthetic vectors. Advanced Drug Delivery Reviews 2015, 81 , 142-160. https://doi.org/10.1016/j.addr.2014.10.031
    85. Sa Hoon Min, Sang Kyu Kwak, Byeong-Su Kim. Atomistic simulation for coil-to-globule transition of poly(2-dimethylaminoethyl methacrylate). Soft Matter 2015, 11 (12) , 2423-2433. https://doi.org/10.1039/C4SM02242D
    86. Lutz Nuhn, Lydia Braun, Iris Overhoff, Annette Kelsch, David Schaeffel, Kaloian Koynov, Rudolf Zentel. Degradable Cationic Nanohydrogel Particles for Stimuli‐Responsive Release of siRNA. Macromolecular Rapid Communications 2014, 35 (24) , 2057-2064. https://doi.org/10.1002/marc.201400458
    87. Theoni K Georgiou. Star polymers for gene delivery. Polymer International 2014, 63 (7) , 1130-1133. https://doi.org/10.1002/pi.4718
    88. Jinming Hu, Guoying Zhang, Zhishen Ge, Shiyong Liu. Stimuli-responsive tertiary amine methacrylate-based block copolymers: Synthesis, supramolecular self-assembly and functional applications. Progress in Polymer Science 2014, 39 (6) , 1096-1143. https://doi.org/10.1016/j.progpolymsci.2013.10.006
    89. Xiaomin Qian, Lixia Long, Zhendong Shi, Chaoyong Liu, Mingzhe Qiu, Jing Sheng, Peiyu Pu, Xubo Yuan, Yu Ren, Chunsheng Kang. Star-branched amphiphilic PLA-b-PDMAEMA copolymers for co-delivery of miR-21 inhibitor and doxorubicin to treat glioma. Biomaterials 2014, 35 (7) , 2322-2335. https://doi.org/10.1016/j.biomaterials.2013.11.039
    90. Ke Miao, Huanhuan Liu, Youliang Zhao. Thermo, pH and reduction responsive coaggregates comprising AB 2 C 2 star terpolymers for multi-triggered release of doxorubicin. Polym. Chem. 2014, 5 (10) , 3335-3345. https://doi.org/10.1039/C3PY01767B
    91. Yu-Lun Lo, Guo-Jing Chen, Tzu-Hwa Feng, Ming-Han Li, Li-Fang Wang. Synthesis and characterization of S-PCL-PDMAEMA for co-delivery of pDNA and DOX. RSC Adv. 2014, 4 (22) , 11089-11098. https://doi.org/10.1039/C3RA46914J
    92. Yan-Fang Li, Marina Rubert, Hüsnü Aslan, Ying Yu, Kenneth A. Howard, Mingdong Dong, Flemming Besenbacher, Menglin Chen. Ultraporous interweaving electrospun microfibers from PCL–PEO binary blends and their inflammatory responses. Nanoscale 2014, 6 (6) , 3392. https://doi.org/10.1039/c3nr06197c
    93. Shereen Tan, Edgar H. H. Wong, Qiang Fu, Jing M. Ren, Adrian Sulistio, Katharina Ladewig, Anton Blencowe, Greg G. Qiao. Azobenzene-Functionalised Core Cross-Linked Star Polymers and their Host–Guest Interactions. Australian Journal of Chemistry 2014, 67 (1) , 173. https://doi.org/10.1071/CH13425
    94. Shu Jie Lam, Adrian Sulistio, Katharina Ladewig, Edgar H. H. Wong, Anton Blencowe, Greg G. Qiao. Peptide-Based Star Polymers as Potential siRNA Carriers. Australian Journal of Chemistry 2014, 67 (4) , 592. https://doi.org/10.1071/CH13525
    95. Yu‐Lun Lo, Yao‐Sheng Wang, Li‐Fang Wang. The Copolymer of Poly(2‐dimethylaminoethyl methacrylate) and Methacrylated Chondroitin Sulfate with Low Cytotoxicity for Gene Delivery. Advanced Healthcare Materials 2013, 2 (11) , 1458-1468. https://doi.org/10.1002/adhm.201200373
    96. Vanessa Loczenski Rose, G. Sebastiaan Winkler, Stephanie Allen, Sanyogitta Puri, Giuseppe Mantovani. Polymer siRNA conjugates synthesised by controlled radical polymerisation. European Polymer Journal 2013, 49 (10) , 2861-2883. https://doi.org/10.1016/j.eurpolymj.2013.06.002
    97. Qijing Chen, Xueteng Cao, Yuanyuan Xu, Zesheng An. Emerging Synthetic Strategies for Core Cross‐Linked Star (CCS) Polymers and Applications as Interfacial Stabilizers: Bridging Linear Polymers and Nanoparticles. Macromolecular Rapid Communications 2013, 34 (19) , 1507-1517. https://doi.org/10.1002/marc.201300487
    98. Chong Sun, Xiaobo Wang, Xujie Yang, Lei Xing, Bo Zhao, Xiaodi Yang, Chun Mao. A label-free electrochemical aptasensor for sensitive thrombin detection in whole blood. Electrochimica Acta 2013, 106 , 327-332. https://doi.org/10.1016/j.electacta.2013.05.100
    99. Stéphanie Deshayes, Andrea M. Kasko. Polymeric biomaterials with engineered degradation. Journal of Polymer Science Part A: Polymer Chemistry 2013, 51 (17) , 3531-3566. https://doi.org/10.1002/pola.26765
    100. Wei Zhou, Weijun Yu, Zesheng An. RAFT emulsion polymerization of styrene mediated by core cross-linked star (CCS) polymers. Polymer Chemistry 2013, 4 (6) , 1921. https://doi.org/10.1039/c2py21074f
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