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Design and Implementation of Two-Dimensional Polymer Adsorption Models: Evaluating the Stability of Candida antarctica Lipase B/Solid-Support Interfaces by QCM-D

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Materials Science & Engineering Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, United States
Center for Biocatalysis and Bioprocessing, Polytechnic Institute of NYU, Brooklyn, New York 11201, United States
*Mailing address: Polytechnic Institute of NYU, Brooklyn, New York 11201, United States (R.G.); Materials Science & Engineering Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, United States (K.L.B.).
Cite this: Biomacromolecules 2013, 14, 2, 377–386
Publication Date (Web):January 4, 2013
https://doi.org/10.1021/bm301557y
Copyright © 2013 American Chemical Society
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Abstract

A two-dimensional model of a solid-supported enzyme catalyst bead is fabricated on a quartz crystal microbalance with dissipation monitoring (QCM-D) sensor to measure in situ interfacial stability and mechanical properties of Candida antarctica Lipase B (CAL B) under varied conditions relating to ring-opening polymerization. The model was fabricated using a dual photochemical approach, where poly(methyl methacrylate) (PMMA) thin films were cross-linked by a photoactive benzophenone monolayer and blended cross-linking agent. This process produces two-dimensional, homogeneous, rigid PMMA layers, which mimic commercial acrylic resins in a QCM-D experiment. Adsorption of CAL B to PMMA in QCM-D under varied buffer ionic strengths produces a viscoelastic enzyme surface that becomes more rigid as ionic strength increases. The rigid CAL B/PMMA interface demonstrates up to 20% desorption of enzyme with increasing trace water content. Increased polycaprolactone (PCL) binding at the enzyme surface was also observed, indicating greater PCL affinity for a more hydrated enzyme surface. The enzyme layer destabilized with increasing temperature, yielding near complete reversible catalyst desorption in the model.

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AFM images, frequency, and dissipation QCM-D data are included in the Supporting Information. This material is available free of charge via the Internet at http://pubs.acs.org.

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This article is cited by 13 publications.

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  3. Hui Huang, Li-li Ding, Hong-qiang Ren, Jin-ju Geng, Ke Xu, and Yan Zhang . Preconditioning of Model Biocarriers by Soluble Pollutants: A QCM-D Study. ACS Applied Materials & Interfaces 2015, 7 (13) , 7222-7230. https://doi.org/10.1021/acsami.5b00324
  4. Logan T. Kearney and John A. Howarter . QCM-Based Measurement of Chlorine-Induced Polymer Degradation Kinetics. Langmuir 2014, 30 (29) , 8923-8930. https://doi.org/10.1021/la501922u
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  7. Gaby Nordendorf, Samuel L. Schafforz, Eireen B. Käkel, Shunyi Miao, Alexander Lorenz. Surface grafted agents with various molecular lengths and photochemically active benzophenone moieties. Physical Chemistry Chemical Physics 2020, 22 (3) , 1774-1783. https://doi.org/10.1039/C9CP05722F
  8. Peng-Cheng Chen, Xiao-Jun Huang, Zhi-Kang Xu. Activation and deformation of immobilized lipase on self-assembled monolayers with tailored wettability. Physical Chemistry Chemical Physics 2015, 17 (20) , 13457-13465. https://doi.org/10.1039/C5CP00802F
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  11. Erick S. Vasquez, Jacquelyn Bowser, Cyprianna Swiderski, Keisha B. Walters, Santanu Kundu. Rheological characterization of mammalian lung mucus. RSC Adv. 2014, 4 (66) , 34780-34783. https://doi.org/10.1039/C4RA05055J
  12. Zhenzhen Yang, Brian J. Ingram, Lynn Trahey. Interfacial Studies of Li-Ion Battery Cathodes Using In Situ Electrochemical Quartz Microbalance with Dissipation. Journal of The Electrochemical Society 2014, 161 (6) , A1127-A1131. https://doi.org/10.1149/2.101406jes
  13. Ming Zhang, Jessica Soto-Rodríguez, I-Cheng Chen, Mustafa Akbulut. Adsorption and removal dynamics of polymeric micellar nanocarriers loaded with a therapeutic agent on silica surfaces. Soft Matter 2013, 9 (42) , 10155. https://doi.org/10.1039/c3sm51692j

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