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Hyaluronan and Phospholipid Association in Biolubrication

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KTH Royal Institute of Technology, School of Chemical Sciences and Engineering, Department of Chemistry, Surface and Corrosion Science, Drottning Kristinas väg 51, SE-10044 Stockholm, Sweden
SP Technical Research Institute of Sweden, SP Chemistry, Materials and Surfaces, Box 5607, SE-114 86 Stockholm, Sweden
Cite this: Biomacromolecules 2013, 14, 12, 4198–4206
Publication Date (Web):October 30, 2013
https://doi.org/10.1021/bm400947v
Copyright © 2013 American Chemical Society
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Abstract

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It is becoming increasingly clear that the outstanding lubrication of synovial joints is achieved by a sophisticated hierarchical structure of cartilage combined with synergistic actions of surface-active components present in the synovial fluid. In this work we focus on the association of two components of the synovial fluid, hyaluronan and dipalmitoyl phosphatidyl choline (DPPC), in bulk solution and at interfaces. We demonstrate that hyaluronan associates with DPPC vesicles and adsorbs to supported DPPC bilayers. The association structures formed at the interface are sufficiently stable to allow sequential adsorption of DPPC and hyaluronan, whereby promoting the formation of thick composite layers of these two components. The lubricating ability of such composite layers was probed by the AFM colloidal probe technique and found to be very favorable with low friction coefficients and high load bearing capacity. With DPPC as the last adsorbed component, a friction coefficient of 0.01 was found up to pressures significantly above what is encountered in healthy synovial joints. Hyaluronan as the last added component increases the friction coefficient to 0.03 and decreases the load bearing capacity somewhat (but still above what is needed in the synovial joint). Our data demonstrate that self-assembly structures formed by hyaluronan and phospholipids at interfaces are efficient aqueous lubricants, and it seems plausible that such self-assembly structures contribute to the exceptional lubrication of synovial joints.

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Adsorption isotherm of hyaluronan on DPPC surface. Reproducibility of AFM measurements of normal forces at different surface coating conditions. This information is available free of charge via the Internet at http://pubs.acs.org/.

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