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Incorporation of a Recombinant Biomineralization Fusion Protein into the Crystalline Lattice of Calcite

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    Incorporation of a Recombinant Biomineralization Fusion Protein into the Crystalline Lattice of Calcite
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    † Department of Materials Engineering and the Russell Berrie Nanotechnology Institute, Technion Israel Institute of Technology, Haifa 32000, Israel
    ‡ INM-Leibniz-Institute for New Materials, Campus D2 2, 66123 Saarbruecken, Germany
    § European Synchrotron Radiation Facility, B.P. 220, 38043 Grenoble Cedex, France
    *E-mail: [email protected]
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    Chemistry of Materials

    Cite this: Chem. Mater. 2014, 26, 17, 4925–4932
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    https://doi.org/10.1021/cm500450s
    Published July 2, 2014
    Copyright © 2014 American Chemical Society
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    Abstract

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    High-resolution synchrotron X-ray powder diffraction (XRD) combined with the Rietveld refinement method and confocal laser scanning microscopy (CLSM) were utilized in this study to elucidate the interaction between a recombinant biomineralization protein (perlucin) fused to green fluorescent protein (GFP) and synthetic calcite. Although recombinant perlucin is insoluble, its solubility was increased via fusion to the highly soluble GFP. We demonstrate that GFP-perlucin derivatives become incorporated into the calcite structure and induce concentration-dependent anisotropic lattice distortions along the host’s c-axis. In contrast, GFP alone is hardly incorporated at all. The observed lattice distortions and peculiar microstructure of the crystals are comparable to those previously observed in biogenic calcite. Taking advantage of biotechnology to optimize individual protein properties, such as the solubility of an otherwise insoluble protein derivative, is a promising route toward the synthesis of new and improved biocomposite materials.

    Copyright © 2014 American Chemical Society

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    Silver stained SDS–PAGE of purified His-tagged GFP and His-tagged GFP-perlucin as well as high-resolution powder XRD pattern of all calcite samples, and modeling results of the 3D structure of perlucin proteins. This material is available free of charge via the Internet at http://pubs.acs.org.

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    Cite this: Chem. Mater. 2014, 26, 17, 4925–4932
    Click to copy citationCitation copied!
    https://doi.org/10.1021/cm500450s
    Published July 2, 2014
    Copyright © 2014 American Chemical Society
    Request reuse permissions

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