DNA-Templated Covalent Coupling of G4 PAMAM Dendrimers

Huajie Liu^†^‡, Thomas Tørring^†^‡, Mingdong Dong^†^§, Christian B. Rosen^†^‡, Flemming Besenbacher^†^§, and Kurt V. Gothelf*^†^‡

Centre for DNA Nanotechnology (CDNA) at The Interdisciplinary Nanoscience Center (iNANO), Department of Chemistry, and Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C, Denmark

J. Am. Chem. Soc., 2010, 132 (51), pp 18054–18056

DOI: 10.1021/ja109677n

Publication Date (Web): December 6, 2010

Copyright © 2010 American Chemical Society

kvg@chem.au.dk, †

Centre for DNA Nanotechnology.

, ‡

Department of Chemistry.

, §

Department of Physics and Astronomy.

Cite this:J. Am. Chem. Soc. 132, 51, 18054-18056

Abstract

Abstract Image

Generation-4 polyamidoamine (PAMAM) dendrimers were surface-functionalized with azides or alkynes and conjugated to one DNA strand. DNA-controlled self-assembly of alternating azide and alkyne dendrimers on a DNA template enabled the coupling of the dendrimers by the azide−alkyne “click” reaction to form covalently coupled dimers, trimers, and tetramers. Polymerization of the DNA−dendrimer conjugates was also demonstrated, as well as assembly in a circular structure on DNA origami and imaging by atomic force microscopy.

Materials and methods, DNA sequences, MALDI−TOF spectra, additional AFM images, and agarose gel characterization. This material is available free of charge via the Internet at http://pubs.acs.org.

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DNA-Templated Covalent Coupling of G4 PAMAM Dendrimers

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