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19F NMR Spectroscopy for the Analysis of RNA Secondary Structure Populations

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University of Innsbruck, Institute of Organic Chemistry, Innrain 52a, 6020 Innsbruck, Austria
[email protected]
Cite this: J. Am. Chem. Soc. 2008, 130, 51, 17230–17231
Publication Date (Web):December 3, 2008
https://doi.org/10.1021/ja806716s
Copyright © 2008 American Chemical Society
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Abstract

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Labeling of RNA with site-specific fluorine atoms has become straightforward in recent years and in particular has become an integrated part of engineered functional RNA with therapeutic potential, e.g. for siRNA technologies. Here, we demonstrate that temperature-dependent one-dimensional 19F NMR spectroscopy of oligoribonucleotides is a powerful tool to obtain direct information on the conformational behavior. The approach is particularly useful for the quantification of monomolecular vs bimolecular secondary structure equilibria.

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Synthesis of rF; analysis of other rF/dF/5FU RNAs/DNAs; UV melting analysis. This material is available free of charge via the Internet at http://pubs.acs.org.

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