Abstract
In this work, we investigated how thermally stable ImON:NaNO and ImNO:NaON pairs are recognized by the Klenow fragment (KF). As a result, these complementary base pairs, especially the ImNO:NaON pair, were recognized selectively due to the four hydrogen bonds between the nucleobases and the shape complementarity of the Im:Na pair similar to the purine:pyrimidine base pair.
Synthesis of noncanonical nucleoside 5′-triphosphates and conditions applied in enzymatic incorporation. This material is available free of charge via the Internet at http://pubs.acs.org.
